【目的】trag(transfer gene G)是利用IVIAT(in vivo induced antigen technology)通量筛选鉴定的猪链球菌2型(Streptococcus suis type 2,SS2)感染相关因子,研究该基因在猪链球菌(Streptococcus suis,SS)中的分布情况,研究康复血清与...【目的】trag(transfer gene G)是利用IVIAT(in vivo induced antigen technology)通量筛选鉴定的猪链球菌2型(Streptococcus suis type 2,SS2)感染相关因子,研究该基因在猪链球菌(Streptococcus suis,SS)中的分布情况,研究康复血清与免疫血清在免疫印迹中的反应性有无,间接证明其在体内感染与体外培养时表达差异。【方法】鉴于我国分离株trag与GenBank公布的SS2北美株89/1591的trag序列有95.8%的同源性,据此设计和合成一对检测引物,对SS2我国江苏及四川流行株、其他临床分离株和参考株及SS1、SS1/2、SS9、SS7及C群猪源链球菌共43株进行PCR扩增。另设计一对引物,扩增5株SS代表菌株trag的完整阅读框,并对扩增产物进行测序。据软件分析后,选择TRAG(Transfer protein G)免疫原性良好的区域片段的核酸设计表达引物,PCR扩增后定向克隆至表达载体pET28a(+)构建表达质粒,表达蛋白转印到PVDF膜上,分别与SS2猪康复血清和猪高免血清反应。【结果】trag在SS2中94%(30/32)阳性,SS9中67%(4/6)阳性,SS7阳性,SS1、SS1/2及C群菌阴性。5株细菌TRAG的氨基酸序列与SS2中国株98HAH33、05ZYH33及北美株89/1591同源性>97%。所获得重组蛋白只能被康复血清识别。【结论】从SS致病株中检出感染相关基因trag,提示该基因可能与SS致病性有关,重组蛋白的免疫转印结果表明,TRAG可能与SS2体内感染相关。展开更多
To solve the deadlock problem of tasks that the interdependence between tasks fails to consider during the course of resource assignment and task scheduling based on the heuristics algorithm, an improved ant colony sy...To solve the deadlock problem of tasks that the interdependence between tasks fails to consider during the course of resource assignment and task scheduling based on the heuristics algorithm, an improved ant colony system （ACS） based algorithm is proposed. First, how to map the resource assignment and task scheduling （RATS） problem into the optimization selection problem of task resource assignment graph （TRAG） and to add the semaphore mechanism in the optimal TRAG to solve deadlocks are explained. Secondly, how to utilize the grid pheromone system model to realize the algorithm based on ACS is explicated. This refers to the construction of TRAG by the random selection of appropriate resources for each task by the user agent and the optimization of TRAG through the positive feedback and distributed parallel computing mechanism of the ACS. Simulation results show that the proposed algorithm is effective and efficient in solving the deadlock problem.展开更多
文摘【目的】trag(transfer gene G)是利用IVIAT(in vivo induced antigen technology)通量筛选鉴定的猪链球菌2型(Streptococcus suis type 2,SS2)感染相关因子,研究该基因在猪链球菌(Streptococcus suis,SS)中的分布情况,研究康复血清与免疫血清在免疫印迹中的反应性有无,间接证明其在体内感染与体外培养时表达差异。【方法】鉴于我国分离株trag与GenBank公布的SS2北美株89/1591的trag序列有95.8%的同源性,据此设计和合成一对检测引物,对SS2我国江苏及四川流行株、其他临床分离株和参考株及SS1、SS1/2、SS9、SS7及C群猪源链球菌共43株进行PCR扩增。另设计一对引物,扩增5株SS代表菌株trag的完整阅读框,并对扩增产物进行测序。据软件分析后,选择TRAG(Transfer protein G)免疫原性良好的区域片段的核酸设计表达引物,PCR扩增后定向克隆至表达载体pET28a(+)构建表达质粒,表达蛋白转印到PVDF膜上,分别与SS2猪康复血清和猪高免血清反应。【结果】trag在SS2中94%(30/32)阳性,SS9中67%(4/6)阳性,SS7阳性,SS1、SS1/2及C群菌阴性。5株细菌TRAG的氨基酸序列与SS2中国株98HAH33、05ZYH33及北美株89/1591同源性>97%。所获得重组蛋白只能被康复血清识别。【结论】从SS致病株中检出感染相关基因trag,提示该基因可能与SS致病性有关,重组蛋白的免疫转印结果表明,TRAG可能与SS2体内感染相关。
文摘To solve the deadlock problem of tasks that the interdependence between tasks fails to consider during the course of resource assignment and task scheduling based on the heuristics algorithm, an improved ant colony system （ACS） based algorithm is proposed. First, how to map the resource assignment and task scheduling （RATS） problem into the optimization selection problem of task resource assignment graph （TRAG） and to add the semaphore mechanism in the optimal TRAG to solve deadlocks are explained. Secondly, how to utilize the grid pheromone system model to realize the algorithm based on ACS is explicated. This refers to the construction of TRAG by the random selection of appropriate resources for each task by the user agent and the optimization of TRAG through the positive feedback and distributed parallel computing mechanism of the ACS. Simulation results show that the proposed algorithm is effective and efficient in solving the deadlock problem.
