电针足三里与内关公孙不同配伍对胃黏膜损伤大鼠NO EGF的影响

目的:观察电针足三里与内关、公孙不同配伍对胃黏膜损伤大鼠表皮生长因子(EGF)、一氧化氮(NO)的影响以了解不同配伍其效应有何差异。方法:60只大鼠随机分为6组,即足三里组、足三里配内关组、足三里配公孙组、足三里配内关公孙组、模型组、空白对照组,每组10只。用无水乙醇按0.6mL/100g灌胃,造成胃黏膜损伤模型。各组检测胃黏膜EGF、血清和胃黏膜N0含量。结果:各电针组胃黏膜EGF含量明显高于模型组(P<0.01),血清和胃黏膜NO含量高于模型组(P<0.05);足三里组、足三里内关组、足三里公孙组之间没有显著差异,而足三里内关公孙组对比这三组均有显著差异。结论:电针足三里与内关、公孙不同配伍能提高胃黏膜EGF含量,促进NO的合成与释放,起到对胃黏膜的保护作用。内关、公孙与足三里单独配伍没有明显地增效作用,而两穴合用时可有明显增效作用。

Effects of three needling manipulations of the right-side Zusanli(ST 36) on brain using functional magnetic resonance imaging

OBJECTIVE: To investigate the effects on the brain using three needling manipulations(twirling, lifting-thrusting, and twirling plus lifting-thrusting)when the right-side Zusanli(ST 36) acupoint was stimulated with needles.METHODS: Seventeen healthy subjects accepted three needling manipulations stimulating the right Zusanli(ST 36) over separate days. Functional magnetic resonance imaging was used to detect changes in the brain during the manipulations, and then the needling sensations were recorded using the MGH acupuncture sensation scale(MASS) after each scan. f MRI data were processed using Statistical Parametric Mapping 8 to analyze the positiveand negative activation in the brain induced by different acupuncture manipulations.RESULTS: The individual needling sensations showed no statistically significant differences among the three manipulations. However, the MASS index showed that lifting-thrusting > twirling plus lifting-thrusting > twirling. Lifting-thrusting activated left premotor cortex, left postcentral,right middle frontal gyrus, left inferior frontal gyrus,right lingual gyrus, left insula, right putamen, bilateral cingulate gyrus and right cerebellum; and deactivated bilateral hippocampus and left caudate.Twirling activated bilateral orbital middle frontal gyrus, left opercular and triangular inferior frontal gyrus, and right middle occipital gyrus; and deactivated bilateral precuneus, right amygdala, left anterior cingulate gyrus, right inferior temporal gyrus,right middle frontal gyrus, right supplementary motor gyrus, and left postcentral. Twirling plus lifting-thrusting activated bilateral postcentral, left inferior occipital gyrus, left insula, left thalamus, left cingulate gyrus, and right putamen; and deactivated right superior frontal gyrus, right superior parietal gyrus, right temporal gyrus, right middle occipital gyrus, right insula, and left lingual gyrus. Pairwise comparisons of the three manipulations showed that signals induced by lifting-thrusting were the strongest, especially in the limbic system,followed by twirling plus lifting-thrusting; twirling alone was the weakest.CONCLUSION: Three methods of needling manipulations similarly activated areas associated with the somatosensory system, vision, cognition, and emotional regulation. This may have significant implications for acupuncture in clinical practice.

ERK信号通路在电针刺激足三里穴和阳陵泉穴减轻大鼠神经病理性痛中的作用

目的探讨细胞外信号调节激酶（ERK）信号通路在电针刺激足三里穴和阳陵泉穴减轻大鼠神经病理性痛中的作用。方法健康成年雄性SD大鼠50只，体重180—220g，鞘内置管成功后，采用随机数字表法，将其随机分为5组（n=10）：假手术组（S组）、CCI组、电针刺激穴位组（EA组）、电针刺激非穴位组（NA．EA组）和ERK抑制剂U0126组（U0126组）。CCI组、EA组、NA-EA组和U0126组采用坐骨神经慢性结扎的方法制备神经病理性痛模型。EA组于术后4d开始电针刺激术侧足三里穴和阳陵泉穴，连续波，频率2Hz，刺激强度≤1．5mA，1次，d，30min／次，连续6d；NA．EA组电针刺激术侧足三里穴旁开5mm和阳陵泉穴旁开5mm处非穴位点。U0126组术后4d开始鞘内注射U0126，5μg／次，2次，d，连续6d。分别于CCI术前1d（To，基础状态）和术后3、5、7、9d（T。）时测定机械刺激缩足反应阈值（MWT）和热刺激缩足潜伏期（TWL）。于术后10d处死大鼠，取术侧脊髓L4-6节段，检测脊髓背角P2X3受体蛋白及其mRNA表达，测定脊髓背角ERK1／2和磷酸化ERK1／2（p-ERKl／2）表达。结果与S组比较，其余4组MWT降低，TWL缩短，脊髓背角P2K受体蛋白及其mRNAp．ERK1／2表达上调（P〈0．01）；与CCI组比较，EA组和U0126组MWT升高，TWL延长，脊髓背角P2X，受体蛋白及其mRNA表达和p-ERKl／2表达下调（P〈0．01），NA—EA组上述指标差异无统计学意义（P〉0．05）；与EA组比较，U0126组MWT升高，TWL延长，脊髓背角P2X受体蛋白及其mRNA表达和p-ERK1／2表达下调（P〈0．01）。结论电针刺激足三里穴和阳陵泉穴可减轻大鼠神经病理性痛，其机制与抑制脊髓ERK信号通路有关。

Effect of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors

OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvasive method for postoperative recovery of bowel functions.METHODS:Forty patients were randomly and evenly assigned into a regular nursing care group(RNC) and an acupoint electric stimulation group(AES).Patients in the RNC group received regular nursing care and patients in the AES group received regular nursing care plus electric stimulation of acupoints.The serum levels of gastrin(GAS),motilin(MOT),and cholecystokinin(CCK),and an electrogastrogram(EGG) of all the patients were evaluated on the first,third,and fifth day after surgery.The time to first flatus after surgery and the number of patients with side effects such as abdominal pain,abdominal distention,and diarrhea were recorded.RESULTS:There were significant differences between the two groups in GAS,MOT,EGG,time to first flatus,abdominal pain,abdominal distention,and diarrhea(P < 0.05).CONCLUSION:Electric stimulation on acupoints could increase levels of GAS and MOT,promote the recovery of gastrointestinal functions,and decrease complications among postoperative senile patients with gastrointestinal tumors.