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Alternate cotton-peanut intercropping:a new approach to increasing productivity and minimizing environmental impact
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作者 CHI Baojie DONG Hezhong 《Journal of Cotton Research》 CAS 2024年第1期101-103,共3页
Recent publications have highlighted the development of an alternate cotton-peanut intercropping as a novel strat-egy to enhance agricultural productivity.In this article,we provide an overview of the progress made in... Recent publications have highlighted the development of an alternate cotton-peanut intercropping as a novel strat-egy to enhance agricultural productivity.In this article,we provide an overview of the progress made in the alternate cotton-peanut intercropping,specifically focusing on its yield benefits,environmental impacts,and the underlying mechanisms.In addition,we advocate for future investigations into the selection or development of appropriate crop varieties and agricultural equipment,pest management options,and the mechanisms of root-canopy interactions.This review is intended to provide a valuable reference for understanding and adopting an alternate intercropping system for sustainable cotton production. 展开更多
关键词 Cotton PEANUT alternate intercropping PRODUCTIVITY
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Pile foundation in alternate layered liquefiable and non-liquefiable soil deposits subjected to earthquake loading
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作者 Praveen Huded M Suresh R Dash 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第2期359-376,共18页
Pile foundations are still the preferred foundation system for high-rise structures in earthquake-prone regions.Pile foundations have experienced failures in past earthquakes due to liquefaction.Research on pile found... Pile foundations are still the preferred foundation system for high-rise structures in earthquake-prone regions.Pile foundations have experienced failures in past earthquakes due to liquefaction.Research on pile foundations in liquefiable soils has primarily focused on the pile foundation behavior in two or three-layered soil profiles.However,in natural occurrence,it may occur in alternative layers of liquefiable and non-liquefiable soil.However,the experimental and/or numerical studies on the layered effect on pile foundations have not been widely addressed in the literature.Most of the design codes across the world do not explicitly mention the effect of sandwiched non-liquefiable soil layers on the pile response.In the present study,the behavior of an end-bearing pile in layered liquefiable and non-liquefiable soil deposit is studied numerically.This study found that the kinematic bending moment is higher and governs the design when the effect of the sandwiched non-liquefied layer is considered in the analysis as opposed to when its effect is ignored.Therefore,ignoring the effect of the sandwiched non-liquefied layer in a liquefiable soil deposit might be a nonconservative design approach. 展开更多
关键词 pile foundation LIQUEFACTION alternately layered soil fixity effect layered effect
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甘肃省陇南市核桃枝枯病菌Alternaria alternate的生物学特性
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作者 邓小芸 高孟春 杨成德 《安徽农业科学》 CAS 2024年第17期133-137,共5页
以核桃枝枯病菌(Alternaria alternate)为对象,研究温度、碳源、氮源、光照、培养基和pH等对菌丝生长、产孢量和孢子萌发的影响。结果表明,pH 7和全黑暗有利于核桃枝枯病菌的菌丝生长、产孢和孢子萌发;25℃有利于菌丝生长,25~30℃有利... 以核桃枝枯病菌(Alternaria alternate)为对象,研究温度、碳源、氮源、光照、培养基和pH等对菌丝生长、产孢量和孢子萌发的影响。结果表明,pH 7和全黑暗有利于核桃枝枯病菌的菌丝生长、产孢和孢子萌发;25℃有利于菌丝生长,25~30℃有利于产孢,20~30℃有利于孢子萌发;最适生长的碳源为可溶性淀粉,氮源为酵母浸粉、KNO_(3)和蛋白胨,最适产孢的碳源和氮源分别为山梨醇和KNO_(3),最适孢子萌发的碳源和氮源分别为乳糖和NH_(4)NO_(3);PSA、OA和PDA培养基适合菌丝生长,LB培养基适合产孢,OA培养基适合孢子萌发。该研究结果为明确核桃枝枯病的发生规律提供理论依据,也为综合防治措施的制定提供参考。 展开更多
关键词 交链格孢菌 生物学特性 测定
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Effect of fertigation frequency on soil nitrogen distribution and tomato yield under alternate partial root-zone drip irrigation 被引量:2
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作者 FENG Xu-yu PU Jing-xuan +5 位作者 LIU Hai-jun WANG Dan LIU Yu-hang QIAO Shu-ting LEI Tao LIU Rong-hao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第3期897-907,共11页
Alternate partial root-zone drip fertigation (ADF) is a combination of alternating irrigation and drip fertigation,with the potential to save water and increase nitrogen (N) fertilizer efficiency.A 2-year greenhouse e... Alternate partial root-zone drip fertigation (ADF) is a combination of alternating irrigation and drip fertigation,with the potential to save water and increase nitrogen (N) fertilizer efficiency.A 2-year greenhouse experiment was conducted to evaluate the effect of different fertigation frequencies on the distribution of soil moisture and nutrients and tomato yield under ADF.The treatments included three ADF frequencies with intervals of 3 days (F3),6 days (F6) and 12 days (F12),and conventional drip fertigation as a control (CK),which was fertilized once every 6 days.For the ADF treatments,two drip tapes were placed 10 cm away on each side of the tomato row,and alternate drip irrigation was realized using a manual valve on the distribution tapes.For the CK treatment,a drip tape was located close to the roots of the tomato plants.The total N application rate of all treatments was 180 kg ha^(-1).The total irrigation amounts applied to the CK treatment were450.6 and 446.1 mm in 2019 and 2020,respectively;and the irrigation amounts applied to the ADF treatments were 60%of those of the CK treatment.The F3 treatment resulted in water and N being distributed mainly in the 0–40-cm soil layer with less water and N being distributed in the 40–60-cm soil layer.The F6 treatment led to 21.0 and 29.0%higher 2-year average concentration of mineral N in the 0–20 and 20–40-cm soil layer,respectively and a 23.0%lower N concentration in the 40–60-cm soil layer than in the CK treatment.The 2-year average tomato yields of the F3,F6,F12,and CK treatments were 107.5,102.6,87.2,and 98.7 t ha^(-1),respectively.The tomato yield of F3 was significantly higher (23.3%) than that in the F12 treatment,whereas there was no significant difference between the F3 and F6 treatment.The F6 treatment resulted in yield similar to the CK treatment,indicating that ADF could maintain tomato yield with a 40%saving in water use.Based on the distribution of water and N,and tomato yield,a fertigation frequency of 6 days under ADF should be considered as a water-saving strategy for greenhouse tomato production. 展开更多
关键词 alternate partial root-zone irrigation drip fertigation soil water soil mineral content tomato yield
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Diversity of Arbuscular Mycorrhizal Fungi Associated with Six Rice Cultivars in Italian Agricultural Ecosystem Managed with Alternate Wetting and Drying 被引量:1
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作者 Veronica VOLPE Franco MAGURNO +2 位作者 Paola BONFANTE Stefano GHIGNONE Erica LUMINI 《Rice science》 SCIE CSCD 2023年第4期348-358,I0028-I0030,共14页
Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was propo... Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa. 展开更多
关键词 alternate wetting and drying system arbuscular mycorrhizal fungi rice molecular diversity virtual taxa
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Generation and Evolution of Cavitation Bubbles in Volume Alternate Cavitation(VAC)
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作者 Shangshuang Chen Yun Wang +4 位作者 Fuzhu Li Shenwei Xue Zhenying Xu Chao Yu Kun Zhang 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2023年第3期241-251,共11页
Cavitation generation methods have been used in multifarious directions because of their diversity,and numerous studies and discussions have been conducted on cavitation generation methods.This study aims to explore t... Cavitation generation methods have been used in multifarious directions because of their diversity,and numerous studies and discussions have been conducted on cavitation generation methods.This study aims to explore the generating mechanism and evolution law of volume alternate cavitation(VAC).In the VAC,liquid water is placed in an airtight container with a variable volume.As the volume alternately changes,the liquid water inside the container continues to cavitate.Then,the mixture turbulence model and in-cylinder dynamic grid model are adopted to conduct computational fluid dynamics simulation of volume alternate cavitation.In the simulation,the cloud images at seven heights on the central axis are monitored,and the phenomenon and mechanism of height and eccentricity are analyzed in detail.By employing the cavitation flow visualization method,the generating mechanism and evolution law of cavitation are revealed.The synergistic effects of experiments and high-speed camera capturing confirm the correctness of the simulation results.In the experiment,the volume change stroke of the airtight container is set to 20 mm,the volume change frequency is 18 Hz,and the shooting frequency of the high-speed camera is set to 10000 FPS.The experimental results indicate that the position of the cavitation phenomenon has a reasonable law during the whole evolution cycle of the cavitation cloud.Also,the volume alternation cycle corresponds to the generation,development,and collapse stages of cavitation bubbles. 展开更多
关键词 Cavitation generation method Volume alternate cavitation(VAC) Generating mechanism Evolution law Computational fluid dynamics(CFD) Cavitation flow visualization(CFV)
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Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape
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作者 Hainan Liu Maosong Pei +5 位作者 Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi Dalong Guo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期863-875,共13页
The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the under... The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries. 展开更多
关键词 GRAPE postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)
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SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation
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作者 Zhibo Yu Jun Wang +11 位作者 Cheng Zhang Qiuna Zhan Leqian Shi Bing Song Danlu Han Jieming Jiang Junwen Huang Xiaolin Ou Zhonghui Zhang Jianbin Lai Qingshun Quinn Li Chengwei Yang 《Molecular Plant》 SCIE CSCD 2024年第9期1392-1406,共15页
Under warm temperatures,plants adjust their morphologies for environmental adaption via precise gene expression regulation.However,the function and regulation of alternative polyadenylation(APA),an important fine-tuni... Under warm temperatures,plants adjust their morphologies for environmental adaption via precise gene expression regulation.However,the function and regulation of alternative polyadenylation(APA),an important fine-tuning of gene expression,remains unknown in plant thermomorphogenesis.In this study,we found that SUMOylation,a critical post-translational modification,is induced by a long-term treat-ment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis.Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes.CPSF100,a key subunit of the CPSF complex for polyadenylation regulation,is SUMOylated by SIZ1.Importantly,we demonstrated that SUMOylation is essential for the function of CPSF1oo in genome-wide polyadenylation site choice during thermomorphogenesis.Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30,increasing the nuclear accumulation of CPsF1oo for polyadenylation regulation.In summary,our study uncovers a regulatory mechanism of APA via SiZ1-mediated SUMOylation in plant thermomorpho-genesis. 展开更多
关键词 alternative polyadenylation CPSF100 SiZ1 SUMOYLATION thermomorphogenesis
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Alternate Reality of the Lady of Shalott
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作者 Tanzim Aziz 《Journal of Literature and Art Studies》 2023年第11期837-841,共5页
The Lady of Shalott is considered to be one of Alfred Lord Tennyson’s masterpieces.The poem conversely demonstrates art and life,the aesthetic and the political,are fully interwoven:the involvement in the social worl... The Lady of Shalott is considered to be one of Alfred Lord Tennyson’s masterpieces.The poem conversely demonstrates art and life,the aesthetic and the political,are fully interwoven:the involvement in the social world which is symbolically the destination of the Lady in the poem.The Lady who is the emblem of such an artwork can be ascribed qualities,for instance,self-containment,objectified otherness,removal from the fluidity of life,and participation in a higher order of existence,for which it is autonomy.Kermode points out that“the Romantic artwork often identifies itself with an emblematic image of autonomy and femininity”,inextricably interlinked.Her mirror shows her only“Shadows of the world”.The Lady’s web flies“Out”and floats“wide”when she“turns round”with“that desire of making an impression upon another mind”,when she seeks to address Lancelot.The mirror reconnects the Lady’s art to the real world beyond her window.Though the artist is unable to see it during the process of production,her artistic web,once out in a wider space than the tower and freed from the loom,is a true representation of outward things,the actions,and events of Camelot.This paper examines that the traditional referentiality of Tennyson’s‘The Lady of Shalott’can be better understood by supplementing the poet’s medieval sources,of little more than tangential relevance to the poem,with Homeric influences.The poem is similar to a fairytale,however,I have given an alternate reality of the Lady of Shallot in my version of a fairytale,where the characters remain the same but the fairytale takes a happy ending rather than a tragic death.My version is different from Tennyson’s since I believe the lady deserves to be jovial.Sir Lancelot was unaware of her fascination and feelings for him,as she could hardly express her feelings due to her imprisonment.She is so taken by him that she stops her work and looks at Camelot,though she risks the mysterious curse.The mirror breaks and her web magically floats out of the window on its own.She realizes that she is cursed. 展开更多
关键词 MEDIEVAL young maiden imprisoned Island of Shalott Sir Lancelot Camelot mirror web mysterious curse wicked witch alternate reality
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Alternating current heating techniques for lithium-ion batteries in electric vehicles:Recent advances and perspectives
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作者 Xinrong Huang Jinhao Meng +5 位作者 Wei Jiang Wenjie Liu Kailong Liu Yipu Zhang Daniel-Ioan Stroe Remus Teodorescu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第9期679-697,共19页
The significant decrease in battery performance at low temperatures is one of the critical challenges that electric vehicles(EVs)face,thereby affecting the penetration rate in cold regions.Alternating current(AC)heati... The significant decrease in battery performance at low temperatures is one of the critical challenges that electric vehicles(EVs)face,thereby affecting the penetration rate in cold regions.Alternating current(AC)heating has attracted widespread attention due to its low energy consumption and uniform heating advantages.This paper introduces the recent advances in AC heating from the perspective of practical EV applications.First,the performance degradation of EVs in low-temperature environments is introduced briefly.The concept of AC heating and its research methods are provided.Then,the effects of various AC heating methods on battery heating performance are reviewed.Based on existing studies,the main factors that affect AC heating performance are analyzed.Moreover,various heating circuits based on EVs are categorized,and their cost,size,complexity,efficiency,reliability,and heating rate are elaborated and compared.The evolution of AC heaters is presented,and the heaters used in brand vehicles are sorted out.Finally,the perspectives and challenges of AC heating are discussed.This paper can guide the selection of heater implementation methods and the optimization of heating effects for future EV applications. 展开更多
关键词 Lithium-ion battery Low temperature alternating current heating HEATER Electric vehicle
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A Neural-network-based Alternative Scheme to Include Nonhydrostatic Processes in an Atmospheric Dynamical Core
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作者 Yang XIA Bin WANG +13 位作者 Lijuan LI Li LIU Jianghao LI Li DONG Shiming XU Yiyuan LI Wenwen XIA Wenyu HUANG Juanjuan LIU Yong WANG Hongbo LIU Ye PU Yujun HE Kun XIA 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第6期1083-1099,I0002,I0003,共19页
Here,a nonhydrostatic alternative scheme(NAS)is proposed for the grey zone where the nonhydrostatic impact on the atmosphere is evident but not large enough to justify the necessity to include an implicit nonhydrostat... Here,a nonhydrostatic alternative scheme(NAS)is proposed for the grey zone where the nonhydrostatic impact on the atmosphere is evident but not large enough to justify the necessity to include an implicit nonhydrostatic solver in an atmospheric dynamical core.The NAS is designed to replace this solver,which can be incorporated into any hydrostatic models so that existing well-developed hydrostatic models can effectively serve for a longer time.Recent advances in machine learning(ML)provide a potential tool for capturing the main complicated nonlinear-nonhydrostatic relationship.In this study,an ML approach called a neural network(NN)was adopted to select leading input features and develop the NAS.The NNs were trained and evaluated with 12-day simulation results of dry baroclinic-wave tests by the Weather Research and Forecasting(WRF)model.The forward time difference of the nonhydrostatic tendency was used as the target variable,and the five selected features were the nonhydrostatic tendency at the last time step,and four hydrostatic variables at the current step including geopotential height,pressure in two different forms,and potential temperature,respectively.Finally,a practical NAS was developed with these features and trained layer by layer at a 20-km horizontal resolution,which can accurately reproduce the temporal variation and vertical distribution of the nonhydrostatic tendency.Corrected by the NN-based NAS,the improved hydrostatic solver at different horizontal resolutions can run stably for at least one month and effectively reduce most of the nonhydrostatic errors in terms of system bias,anomaly root-mean-square error,and the error of the wave spatial pattern,which proves the feasibility and superiority of this scheme. 展开更多
关键词 neural network nonhydrostatic alternative scheme atmospheric model dynamical core
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Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing
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作者 Yinqing Yang Lei Zhang +7 位作者 Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin Hou Feng Cheng 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期961-970,共10页
Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,ha... Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,has been widely used as a common reference in biological research.Although its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 transcripts.Additionally,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold stress.Meanwhile,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS analysis.Overall,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms. 展开更多
关键词 Brassica oleracea Oxford nanopore technologies Gene annotation alternative splicing
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Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain
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作者 Yi Jiang Wenli Tao +2 位作者 Weiyang Zhang Zhiqin Wang Jianchang Yang 《The Crop Journal》 SCIE CSCD 2024年第1期262-270,共9页
Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breedin... Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG. 展开更多
关键词 BRASSINOSTEROIDS Inferior grain Lysine biosynthesis Rice Wetting alternating with partial drying
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2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-Glucoside modulates CHEK2 and CCND1 alternative splicing to inhibit MCF-7 cells proliferation
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作者 Hui Shen You-Zhi Zhang +5 位作者 Peng-Yu Wang Shuo Zhang Huan Pan Bei-Bei Liu Long-Sheng Xu Jian-Fen Shen 《Traditional Medicine Research》 2024年第1期33-46,共14页
Background:In our previous study,we observed a synergistic effect of 2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-glucoside combined with adriamycin to induce apoptosis in MCF-7 breast cancer cells.However,the underlying me... Background:In our previous study,we observed a synergistic effect of 2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-glucoside combined with adriamycin to induce apoptosis in MCF-7 breast cancer cells.However,the underlying mechanisms of epigenetic modifications,such as alternative splicing,have not been explored.In this study,we aimed to investigate the mechanism by which THSG inhibits MCF-7 cell proliferation using full-length transcriptome sequencing.Methods:First,cell viability was examined using the methyl thiazolyl tetrazolium method and full-length transcriptome sequencing was performed to identify genes and pathways.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were used to identify the principal pathways and targets of THSG.Flow cytometry analysis of cell cycle distribution was performed.Meanwhile,the analysis of alternative splicing and domains of the key proteins was conducted.Quantitative polymerase chain reaction and western blotting were performed for verification.Results:THSG showed significant cytotoxic activity in MCF-7 cells.Full-length transcriptome sequencing revealed differential alternative splicing with 173 upregulated and 263 downregulated genes.Further analysis identified distinct differential expression of genes(CHEK2-211 and CCND1-201)involved in the cell cycle in the THSG-treated group.Subsequently,alternative splicing types of CHEK2(mutually exclusive exon)and CCND1(intron retention).We found that THSG downregulated mRNA expression,as confirmed by quantitative polymerase chain reaction analysis.Interestingly,protein structural analysis revealed that THSG treatment led to the generation of CHK2-211,which was the result of a mutation in the amino acid residues(GLU-150,ASN-151)of the CHEK2 domain(VAL-150,GLY-151).and CyclinD1-201 were obtained when an amino acid(ASP-267)in the domain was lost in CyclinD1.Moreover,molecular docking analysis demonstrated that the domains of key proteins could bind THSG more effectively,with no difference in affinity.Western blotting confirmed that THSG inhibited the expression of CHK2 and CyclinD1.Conclusion:THSG modulated the alternative splicing of CHEK2 and CCND1 by inducing G0/G1 cell cycle arrest,consequently suppressing MCF-7 cell proliferation. 展开更多
关键词 THSG breast cancer full-length transcriptome sequencing alternative splicing
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Workshop “Navigating the Transition: Standards Powering the Journey of Alternative Fuel Infrastructure”
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《China Standardization》 2024年第2期67-67,共1页
April 18, Brussels, Belgium In light of recent significant regulatory changes within the European Union impacting the transport sector, such as the Alternative Fuels Infrastructure Regulation(AFIR), Re Fuel EU and Fue... April 18, Brussels, Belgium In light of recent significant regulatory changes within the European Union impacting the transport sector, such as the Alternative Fuels Infrastructure Regulation(AFIR), Re Fuel EU and Fuel EU Maritime, the role of European standardization becomes increasingly critical in facilitating the transition towards the deployment of alternative fuels. 展开更多
关键词 TRANSITION alternATIVE MARITIME
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Suppression of stimulated Brillouin and Raman scatterings using an alternating frequency laser and transverse magnetic fields
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作者 程瑞锦 李晓旬 +11 位作者 王清 刘德基 黄卓明 吕帅宇 周远志 张舒童 李雪铭 陈祖杰 王强 刘占军 曹莉华 郑春阳 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期545-553,共9页
A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allow... A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allows the laser frequency to change discretely and alternately over time. The suppression of SBS is significant as long as the AF difference is greater than the linear growth rate of SBS or the alternating time of the laser frequency is shorter than the linear growth time of SBS. However, the AF laser proves ineffective in suppressing SRS, which usually has a much higher linear growth rate than SBS. To remedy that, a transverse magnetic field is included to suppress the SRS instability. The electrons trapped in the electron plasma waves(EPWs) of SRS can be accelerated by the surfatron mechanism in a transverse magnetic field and eventually detrapped. While continuously extracting energy from EPWs, the EPWs are dissipated and the kinetic inflation of SRS is suppressed. The one-dimensional particle-in-cell simulation results show that both SBS and SRS can be effectively suppressed by combining the AF laser with a transverse magnetic field with tens of Tesla. The total reflectivity can be dramatically reduced by more than one order of magnitude. These results provide a potential reference for controlling SBS and SRS under the related parameters of inertial confinement fusion. 展开更多
关键词 stimulated Brillouin scattering stimulated Raman scattering alternating frequency laser transverse magnetic field
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Use of Topical Complementary and Alternative Medicines (CAM) in Diabetic Septic Foot Disease in Makkah Region, Western of Saudi Arabia, an Exploratory Study
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作者 Mohamed Elamin Salih Hatim Alrizqi +5 位作者 Mohammed Hussain Abdullah Alshumrani Ismail Alhasani Ali Alkhamisi Hamzah Alsayed Fahad Alqarni 《Journal of Biosciences and Medicines》 2024年第3期245-258,共14页
Diabetes is a chronic condition that is significantly impacted daily by environment. At least one in five Saudis, suffer from diabetes. “Diabetes is a pandemic of unprecedented magnitude spiraling out of control” (B... Diabetes is a chronic condition that is significantly impacted daily by environment. At least one in five Saudis, suffer from diabetes. “Diabetes is a pandemic of unprecedented magnitude spiraling out of control” (Boulton). Saudi Arabia ranks the second highest in the Middle East, and is the seventh in the world for the prevalence of diabetes (WHO). Diabetes mellitus (DM) has been found to be related to high mortality, morbidity accompanied by poor general health and lower quality of life. Current Saudi diabetes patients’ behaviors, practices, and beliefs regarding foot health problems such as ulcers, are not well investigated. Diabetic patients frequently utilize natural remedies and Herbs for self-medication as a part of complementary and alternative Medicine. Aim of study: To explore the widespread use of the alternative remedies in the local communities of the Western Saudi Arabia, the drives behind using and to focus on the potential adverse effects following their usage. Method: A descriptive, cross-sectional study of 386 diabetic patients with feet problems in Makkah Region, West of Saudi Arabia during the period April - June 2023. Results: Out of the 531 participants, only 386 participants reported diabetic foot disorder (DFD). Most of the participant were above 65 years of age group (24.61 %). Male (52.08%) more than female (47.92%) in the research group. All of the participants had diabetic foot disorders at least once. The prevalence of complementary and alternative medicines (CAM) use among them was 67.88%, as solitary or in combination with hospital medicines. Honey headed the list followed by myrrh and black seeds. Acquaintances advice (59.54%) was the most reason for using the alternative medicine. 60.30% of Diabetic foot patient complained of adverse effects during CAM remedies use. Conclusion: Consumption of CAM remedies among diabetic patients for Diabetic Foot Disorder (DFD) is common. Honey headed the list followed by myrrh and black seeds. Additional wide-scale research is required to establish their actual efficacy, safety and potential adverse out-comes and to generalize the outcomes. 展开更多
关键词 Diabetic Foot Ulcer Diabetes Complementary and alternative Medicine CAM West of Saudi Arabia
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Diagnosing ratio of electron density to collision frequency of plasma surrounding scaled model in a shock tube using low-frequency alternating magnetic field phase shift
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作者 吴明兴 谢楷 +3 位作者 刘艳 徐晗 张宝 田得阳 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第5期519-530,共12页
A non-contact low-frequency(LF)method of diagnosing the plasma surrounding a scaled model in a shock tube is proposed.This method utilizes the phase shift occurring after the transmission of an LF alternating magnetic... A non-contact low-frequency(LF)method of diagnosing the plasma surrounding a scaled model in a shock tube is proposed.This method utilizes the phase shift occurring after the transmission of an LF alternating magnetic field through the plasma to directly measure the ratio of the plasma loop average electron density to collision frequency.An equivalent circuit model is used to analyze the relationship of the phase shift of the magnetic field component of LF electromagnetic waves with the plasma electron density and collision frequency.The applicable range of the LF method on a given plasma scale is analyzed.The upper diagnostic limit for the ratio of the electron density(unit:m^(-3))to collision frequency(unit:Hz)exceeds 1×10^(11),enabling an electron density to exceed 1×10^(20)m^(-3)and a collision frequency to be less than 1 GHz.In this work,the feasibility of using the LF phase shift to implement the plasma diagnosis is also assessed.Diagnosis experiments on shock tube equipment are conducted by using both the electrostatic probe method and LF method.By comparing the diagnostic results of the two methods,the inversion results are relatively consistent with each other,thereby preliminarily verifying the feasibility of the LF method.The ratio of the electron density to the collision frequency has a relatively uniform distribution during the plasma stabilization.The LF diagnostic path is a loop around the model,which is suitable for diagnosing the plasma that surrounds the model.Finally,the causes of diagnostic discrepancy between the two methods are analyzed.The proposed method provides a new avenue for diagnosing high-density enveloping plasma. 展开更多
关键词 low-frequency alternating magnetic field phase shift shock-tube plasma diagnosis electron density collision frequency
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Molecular targets and mechanisms of different aberrant alternative splicing in metastatic liver cancer
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作者 De-Yi Geng Qing-Shan Chen +7 位作者 Wan-Xian Chen Lin-Sa Zhou Xiao-Sha Han Qi-Hu Xie Geng-Hong Guo Xue-Fen Chen Jia-Sheng Chen Xiao-Ping Zhong 《World Journal of Clinical Oncology》 2024年第4期531-539,共9页
Metastasis remains a major challenge in the successful management of malignant diseases.The liver is a major site of metastatic disease and a leading cause of death from gastrointestinal malignancies such as colon,sto... Metastasis remains a major challenge in the successful management of malignant diseases.The liver is a major site of metastatic disease and a leading cause of death from gastrointestinal malignancies such as colon,stomach,and pancreatic cancers,as well as melanoma,breast cancer,and sarcoma.As an important factor that influences the development of metastatic liver cancer,alternative splicing drives the diversity of RNA transcripts and protein subtypes,which may provide potential to broaden the target space.In particular,the dysfunction of splicing factors and abnormal expression of splicing variants are associated with the occurrence,progression,aggressiveness,and drug resistance of cancers caused by the selective splicing of specific genes.This review is the first to provide a detailed summary of the normal splicing process and alterations that occur during metastatic liver cancer.It will cover the role of alternative splicing in the mechanisms of metastatic liver cancer by examining splicing factor changes,abnormal splicing,and the contribution of hypoxia to these changes during metastasis. 展开更多
关键词 alternative splicing CARCINOMA HEPATOCELLULAR Metastasic Liver neoplasms PROGNOSIS
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Progression of Corneal Astigmatism after Phaco-Alternative at the University Hospital Center of the Oueme-Plateau Department in Porto-Novo
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作者 Chakiratou Olaïdé Adoukê Abouki Edjrokinto Ahomadegbe Djrodéhou Espérancia Neuilly +4 位作者 Issifou Amadou Alfa Bio Nestor Aïgbè Soulé Alamou Lisette Odoulami Ignace Sounouvou 《Open Journal of Ophthalmology》 2024年第3期313-323,共11页
Objective: To study the postoperative evolution of corneal astigmatism following phaco-alternative at the University Hospital Center of Ouémé-Plateau (UHC-OP) in Porto-Novo. Method: This was a prospective, c... Objective: To study the postoperative evolution of corneal astigmatism following phaco-alternative at the University Hospital Center of Ouémé-Plateau (UHC-OP) in Porto-Novo. Method: This was a prospective, cross-sectional study with a descriptive and analytical purpose conducted from April 19 to September 20, 2021;a period of 6 months. The study included all patients presenting with senile or non-senile cataracts without a history of corneal trauma and who underwent phaco-alternative during the study period. Results: A total of 62 eyes underwent phaco-alternative. The average age was 63 ± 12 years with a male predominance of 59.7%. Phaco-alternative was performed with 74.2% linear incision and 25.8% smile incision. Perioperative incidents were mainly capsular ruptures with vitreous loss occurring in 8.1% of cases. The mean preoperative astigmatism was 1.29 ± 1.5 D with an axis of 180˚ ± 20˚, indicating with-the-rule astigmatism. The mean postoperative astigmatism was 2.2 ± 1.5 D with an axis of 90˚ ± 20˚, indicating against-the-rule astigmatism. The mean induced astigmatism at Day 30 was 1.4 ± 1.2 D for smile incision and 1.8 ± 1.2 D for linear incision. Conclusion: Phaco-alternative yields good results with few complications but remains astigmatogenic. The smile incision appears to be less astigmatogenic. 展开更多
关键词 Cataract Phaco-alternative Smile Incision ASTIGMATISM
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