The lack of standard to electronic circuits modeling made possible the development of many tools and modeling languages for electronic circuits. In this way, several tools to be used on different descriptions stage of...The lack of standard to electronic circuits modeling made possible the development of many tools and modeling languages for electronic circuits. In this way, several tools to be used on different descriptions stage of the designs are necessary. This paper presents a tool called SF^2HDL （Stateflow to Hardware Description Language or State Transition Table） that translates a finite state machine on state transition diagram representation, described by Stateflow tool, into an input file standard for TABELA program or into a file behavioral VHDL （Very High Speed Integrated Circuits Hardware Description Language） directly. The TABELA program was used to optimization this finite state machine. After that, the TAB2VHDL program was used to generate the VHDL code on register transfer level, what permits comparisons with results obtained by synthesis. The finite state machine must be described by Mealy model and the user can describe the machine on high level abstraction using all Simulink supports. The tool was very efficient on computational cost and it made translation of several cases, for the two VHDL description models. Every state machine translated was simulated and implemented on device EP2C20F484C7 using Quartus II environment.展开更多
目的研究人肝癌细胞在高密度微载体培养时的生长条件与培养方式,建立实验室规模高效、简便培养人肝癌细胞的方法。方法应用微载体CultiSpher-S和Cytodex-1在1 L SuperSpinner搅拌瓶中培养人肝癌细胞THCC-98,采用批式换液连续培养的方式...目的研究人肝癌细胞在高密度微载体培养时的生长条件与培养方式,建立实验室规模高效、简便培养人肝癌细胞的方法。方法应用微载体CultiSpher-S和Cytodex-1在1 L SuperSpinner搅拌瓶中培养人肝癌细胞THCC-98,采用批式换液连续培养的方式,对细胞生长及营养物质葡萄糖利用、代谢产物乳酸生成进行分析。结果人肝癌细胞THCC-98在CultiSpher-S和Cytodex-1培养中葡萄糖日消耗量高达2 mmol/106细胞(2.17和2.3),乳酸日生成率为0.75～1.08 mmol/106细胞,最高浓度为9 mmol/L(9.2比9.5)。通过实施批式换液连续培养,换液0.3～0.9 V/d,可使细胞最高密度达(1.04比0.89)×107cells/ml。就两种微载体培养比较,CultiSpher-S由于具有更大的表面积/体积比,提供给细胞更大的生长空间,其所能达到的细胞密度高且营养物质利用更为有效。结论用1 L SuperSpinner搅拌瓶Cultisphere微载体批式换液连续高密度培养人肝癌细胞,细胞密度可达107cells/ml,批式收获细胞>1010。展开更多
文摘The lack of standard to electronic circuits modeling made possible the development of many tools and modeling languages for electronic circuits. In this way, several tools to be used on different descriptions stage of the designs are necessary. This paper presents a tool called SF^2HDL （Stateflow to Hardware Description Language or State Transition Table） that translates a finite state machine on state transition diagram representation, described by Stateflow tool, into an input file standard for TABELA program or into a file behavioral VHDL （Very High Speed Integrated Circuits Hardware Description Language） directly. The TABELA program was used to optimization this finite state machine. After that, the TAB2VHDL program was used to generate the VHDL code on register transfer level, what permits comparisons with results obtained by synthesis. The finite state machine must be described by Mealy model and the user can describe the machine on high level abstraction using all Simulink supports. The tool was very efficient on computational cost and it made translation of several cases, for the two VHDL description models. Every state machine translated was simulated and implemented on device EP2C20F484C7 using Quartus II environment.
文摘目的研究人肝癌细胞在高密度微载体培养时的生长条件与培养方式,建立实验室规模高效、简便培养人肝癌细胞的方法。方法应用微载体CultiSpher-S和Cytodex-1在1 L SuperSpinner搅拌瓶中培养人肝癌细胞THCC-98,采用批式换液连续培养的方式,对细胞生长及营养物质葡萄糖利用、代谢产物乳酸生成进行分析。结果人肝癌细胞THCC-98在CultiSpher-S和Cytodex-1培养中葡萄糖日消耗量高达2 mmol/106细胞(2.17和2.3),乳酸日生成率为0.75～1.08 mmol/106细胞,最高浓度为9 mmol/L(9.2比9.5)。通过实施批式换液连续培养,换液0.3～0.9 V/d,可使细胞最高密度达(1.04比0.89)×107cells/ml。就两种微载体培养比较,CultiSpher-S由于具有更大的表面积/体积比,提供给细胞更大的生长空间,其所能达到的细胞密度高且营养物质利用更为有效。结论用1 L SuperSpinner搅拌瓶Cultisphere微载体批式换液连续高密度培养人肝癌细胞,细胞密度可达107cells/ml,批式收获细胞>1010。
