目的探讨m1A RNA甲基化相关基因和血浆m1A甲基化水平对结肠腺癌(colorectal adenocarcinoma,COAD)的诊断效能,为COAD早期诊断提供新的方案。方法通过UALCAN、The Human Protein Atlas和TCGA-GTEx数据库,分析COAD组织和正常结肠组织中m1...目的探讨m1A RNA甲基化相关基因和血浆m1A甲基化水平对结肠腺癌(colorectal adenocarcinoma,COAD)的诊断效能,为COAD早期诊断提供新的方案。方法通过UALCAN、The Human Protein Atlas和TCGA-GTEx数据库,分析COAD组织和正常结肠组织中m1A相关基因mRNA和蛋白水平的差异表达。利用ELISA法检测收集于我院初诊的COAD患者和正常人血浆中m1A甲基化水平。结合COAD临床病理特征分析m1A甲基化对COAD的诊断效能。结果m1A编码器和读码器基因的蛋白水平和mRNA水平在COAD组织中的表达显著上调,其中以TRMT6和TRMT10C两个编码器表达升高最为显著。两个编码器基因均可作为COAD诊断,尤其是早期诊断标志物,且其AUC均达到0.9以上。m1A总体甲基化水平在COAD血浆中明显升高,并可作为早期COAD的诊断标志物。结论m1A编码器基因和血浆m1A在COAD中明显升高,有望成为一种新的早期COAD诊断标志物。展开更多
The paper presents the neural decoding result of finger or wrist movements using the primary motor cortex(M1)neural activities prior to its movement.It is well known that the observations of motor commands in brain ar...The paper presents the neural decoding result of finger or wrist movements using the primary motor cortex(M1)neural activities prior to its movement.It is well known that the observations of motor commands in brain are in advance before motor movements in the central nerve system.Readiness potential(RP)for electroencephalogram(EEG)has become an important domain of research.Likewise,pre-movement neural responses in M1 primary motor cortex have been observed.The neural activity data before 1 s.were used for neural decoding when the actual movements happened around 1 s.The obtained decoding accuracy in novel method reaches as high as 95% with 30 randomly selected neurons.展开更多
More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from Na-methyladeno...More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from Na-methyladenosine （m6A）, N1-methyladenosine （mXA） has been found as a reversible modification in tRNA and mRNA. mlA occurs at positions 9, 14, and 58 of tRNA, with m1A58 being critical for tRNA stability. Other than the hundreds of m1A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m1A also identifies 〉 20 m1A sites in mitochondrial genes, m1A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of mlA in mRNA and tRNA, covering high-throughput sequencing methods developed for m1A methylome, m1A-related enzymes （writers and erasers）, as well as its functions in mRNA and tRNA.展开更多
The structures and hydrogen storage properties of sodium atoms decorated B6 clusters are investigated by the B3LYP method with a 6-311＋G （d, p） basis set. For NamB6 （m = 1-3） clusters, Na atoms are always incline...The structures and hydrogen storage properties of sodium atoms decorated B6 clusters are investigated by the B3LYP method with a 6-311＋G （d, p） basis set. For NamB6 （m = 1-3） clusters, Na atoms are always inclined to separate far enough from each other and not cluster together on a B6 cluster surface so that each Na atom has sufficient space to bind hydrogen molecules. The hydrogen storage gravimetric density of a two Na atoms decorated B6 cluster is 17.91 wt% with an adsorption energy per H2 molecule （AAE/H2） of 0.6851 kcal.mo1^-1. The appropriate AAE/H2 and preferable gravimetric density of the two Na atoms decorated B6 cluster complex indicate that it is feasible for hydrogen storage application in ambient conditions.展开更多
Given the statistical gaps in material flow among provinces in China, a method was introduced to estimate regional physical imports and exports （RPIE）, which includes international and interregional imports/ exports...Given the statistical gaps in material flow among provinces in China, a method was introduced to estimate regional physical imports and exports （RPIE）, which includes international and interregional imports/ exports. This method uses provincial monetary input- output tables （MIOT） and international trade statistics. A coefficient matrix representing correlations between monetary value and physical mass for years 2000-2009 was obtained based on a detailed commodity classification and 22 material production sectors in MIOT. With the coefficient matrix as reference, RPIE was measured. Pilot calculation of both regional physical trade balance and domestic material consumption, as well as a brief analysis of these methods, were conducted using 2002 data.展开更多
Most apple orchards in the apple production districts in China were densely planted with vigorous rootstocks during the 1980 s. These orchards have suffered micro-environmental deterioration and loss of fruit quality ...Most apple orchards in the apple production districts in China were densely planted with vigorous rootstocks during the 1980 s. These orchards have suffered micro-environmental deterioration and loss of fruit quality because of the closed canopy. Modification of the denselyplanted orchards is a priority in current apple production. Intermediate thinning is a basic technique used to transform densely-planted apple orchards in China. Our goal was to provide theoretical basis for studying the effect of thinning on the efficiency of photosynthetically active radiation(PAR),fruit quality, and yield. We measured leaf area, solar radiation, and leaf air exchange at different tree canopy levels and by fitting relevant photosynthetic models, vertical distribution characteristics of leaf photosynthetic potentials and PAR were analyzed in various levels within canopies in densely-planted and intermediately-thinned orchards. Intermediate thinning significantly improved the radiant environment inside the canopies. PAR distribution within the canopies in the intermediately-thinned orchard was better distributed than in the densely-planted orchards. The invalid space under 30.0% of relative photosynthetically active radiation(PAR_r) was nearly zero in the intermediately-thinned orchard; but minimum PARr was 17.0% and the space under 0.30 of the relative height of the canopy was invalid for photosynthesis in the densely-planted orchard. The leaf photosynthetic efficiency in the intermediately-thinned orchard was improved. Photosynthetic rates(P_n) at the middle and bottom levels of the canopy, respectively, were increased by 7.80% and 10.20% in the intermediately-thinned orchard. Leaf development, which influences photosynthetic potential, was closely related to the surrounding micro-environment, especially light. Leaf photosynthetic potentials were correlated with leaf nitrogen content(N_1) and specific leaf weight(M_1) at various levels of canopies. Compared with the densely-planted orchard, the photosynthetic capacity parameters, such as maximum carboxylation rate(CE_(max)) and maximum electron transfer rate(J_(max)), significantly increased in the intermediately-thinned orchard. Leaf photosynthetic potentials mainly depended on Nl and Nl was closely related to PARr. Leaf photosynthetic potentials and PAR_r can be assessed using spatial distribution patterns of relative leaf nitrogen content(N_(lr)).展开更多
A periodic difference predator-prey model with Holling-（m ＋ 1）（m 〉 2） type functional response and impulses is established. Sufficient conditions are derived for the existence of periodic solutions by using a co...A periodic difference predator-prey model with Holling-（m ＋ 1）（m 〉 2） type functional response and impulses is established. Sufficient conditions are derived for the existence of periodic solutions by using a continuation theorem in coincidence degree.展开更多
文摘目的探讨m1A RNA甲基化相关基因和血浆m1A甲基化水平对结肠腺癌(colorectal adenocarcinoma,COAD)的诊断效能,为COAD早期诊断提供新的方案。方法通过UALCAN、The Human Protein Atlas和TCGA-GTEx数据库,分析COAD组织和正常结肠组织中m1A相关基因mRNA和蛋白水平的差异表达。利用ELISA法检测收集于我院初诊的COAD患者和正常人血浆中m1A甲基化水平。结合COAD临床病理特征分析m1A甲基化对COAD的诊断效能。结果m1A编码器和读码器基因的蛋白水平和mRNA水平在COAD组织中的表达显著上调,其中以TRMT6和TRMT10C两个编码器表达升高最为显著。两个编码器基因均可作为COAD诊断,尤其是早期诊断标志物,且其AUC均达到0.9以上。m1A总体甲基化水平在COAD血浆中明显升高,并可作为早期COAD的诊断标志物。结论m1A编码器基因和血浆m1A在COAD中明显升高,有望成为一种新的早期COAD诊断标志物。
基金MKE(the Ministry of Knowledge Economy),Korea,under the ITRC(Infor mation Technology Research Center)support program supervised by the NIPA(National ITIndustry Promotion Agency)(NIPA-2011-C1090-1121-0010)
文摘The paper presents the neural decoding result of finger or wrist movements using the primary motor cortex(M1)neural activities prior to its movement.It is well known that the observations of motor commands in brain are in advance before motor movements in the central nerve system.Readiness potential(RP)for electroencephalogram(EEG)has become an important domain of research.Likewise,pre-movement neural responses in M1 primary motor cortex have been observed.The neural activity data before 1 s.were used for neural decoding when the actual movements happened around 1 s.The obtained decoding accuracy in novel method reaches as high as 95% with 30 randomly selected neurons.
基金supported by the National Basic Research Program of China (Grant Nos. 2016YFC0900302 and 2017YFA0505201)the National Natural Science Foundation of China (Grant No. 21432002)
文摘More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from Na-methyladenosine （m6A）, N1-methyladenosine （mXA） has been found as a reversible modification in tRNA and mRNA. mlA occurs at positions 9, 14, and 58 of tRNA, with m1A58 being critical for tRNA stability. Other than the hundreds of m1A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m1A also identifies 〉 20 m1A sites in mitochondrial genes, m1A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of mlA in mRNA and tRNA, covering high-throughput sequencing methods developed for m1A methylome, m1A-related enzymes （writers and erasers）, as well as its functions in mRNA and tRNA.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.11264020 and 11364023)the Science Foundation of the Educational Committee of Jiangxi Province,China (Grant Nos.GJJ12463,11530,and 11540)+1 种基金the Doctoral Startup Fund of Jinggangshan University,China (Grant No.JZB11003)the Key Subject of Atomic and Molecular Physics in Jiangxi Province,China (Grant No.2011-2015)
文摘The structures and hydrogen storage properties of sodium atoms decorated B6 clusters are investigated by the B3LYP method with a 6-311＋G （d, p） basis set. For NamB6 （m = 1-3） clusters, Na atoms are always inclined to separate far enough from each other and not cluster together on a B6 cluster surface so that each Na atom has sufficient space to bind hydrogen molecules. The hydrogen storage gravimetric density of a two Na atoms decorated B6 cluster is 17.91 wt% with an adsorption energy per H2 molecule （AAE/H2） of 0.6851 kcal.mo1^-1. The appropriate AAE/H2 and preferable gravimetric density of the two Na atoms decorated B6 cluster complex indicate that it is feasible for hydrogen storage application in ambient conditions.
文摘Given the statistical gaps in material flow among provinces in China, a method was introduced to estimate regional physical imports and exports （RPIE）, which includes international and interregional imports/ exports. This method uses provincial monetary input- output tables （MIOT） and international trade statistics. A coefficient matrix representing correlations between monetary value and physical mass for years 2000-2009 was obtained based on a detailed commodity classification and 22 material production sectors in MIOT. With the coefficient matrix as reference, RPIE was measured. Pilot calculation of both regional physical trade balance and domestic material consumption, as well as a brief analysis of these methods, were conducted using 2002 data.
基金supported by the National Modern Apple Technical System Program of the China Ministry of Agriculture(CARS-28)National Major Program of Research and Development Plan(2016YFD0201100)+1 种基金the‘12th Five-years Plan’National Science and Technology Support Project in Rural Areas(2011BAD32B03-02)the Natural Science Foundation Project of Shandong Province,P.R.China(ZR2009M015)
文摘Most apple orchards in the apple production districts in China were densely planted with vigorous rootstocks during the 1980 s. These orchards have suffered micro-environmental deterioration and loss of fruit quality because of the closed canopy. Modification of the denselyplanted orchards is a priority in current apple production. Intermediate thinning is a basic technique used to transform densely-planted apple orchards in China. Our goal was to provide theoretical basis for studying the effect of thinning on the efficiency of photosynthetically active radiation(PAR),fruit quality, and yield. We measured leaf area, solar radiation, and leaf air exchange at different tree canopy levels and by fitting relevant photosynthetic models, vertical distribution characteristics of leaf photosynthetic potentials and PAR were analyzed in various levels within canopies in densely-planted and intermediately-thinned orchards. Intermediate thinning significantly improved the radiant environment inside the canopies. PAR distribution within the canopies in the intermediately-thinned orchard was better distributed than in the densely-planted orchards. The invalid space under 30.0% of relative photosynthetically active radiation(PAR_r) was nearly zero in the intermediately-thinned orchard; but minimum PARr was 17.0% and the space under 0.30 of the relative height of the canopy was invalid for photosynthesis in the densely-planted orchard. The leaf photosynthetic efficiency in the intermediately-thinned orchard was improved. Photosynthetic rates(P_n) at the middle and bottom levels of the canopy, respectively, were increased by 7.80% and 10.20% in the intermediately-thinned orchard. Leaf development, which influences photosynthetic potential, was closely related to the surrounding micro-environment, especially light. Leaf photosynthetic potentials were correlated with leaf nitrogen content(N_1) and specific leaf weight(M_1) at various levels of canopies. Compared with the densely-planted orchard, the photosynthetic capacity parameters, such as maximum carboxylation rate(CE_(max)) and maximum electron transfer rate(J_(max)), significantly increased in the intermediately-thinned orchard. Leaf photosynthetic potentials mainly depended on Nl and Nl was closely related to PARr. Leaf photosynthetic potentials and PAR_r can be assessed using spatial distribution patterns of relative leaf nitrogen content(N_(lr)).
基金Supported by the National Natural Science Foundation of China(No.10571064)the National Sciences Foundation of Guangdong Province(No.04010364)
文摘A periodic difference predator-prey model with Holling-（m ＋ 1）（m 〉 2） type functional response and impulses is established. Sufficient conditions are derived for the existence of periodic solutions by using a continuation theorem in coincidence degree.
