Disruption of mitochondrial reactive oxygen species （mitoROS） plays a major role in cancer cell apoptosis. Here, we designed a core/shell-structured mitochondriatargeting upconversion-based nano-photosensitizer （TP...Disruption of mitochondrial reactive oxygen species （mitoROS） plays a major role in cancer cell apoptosis. Here, we designed a core/shell-structured mitochondriatargeting upconversion-based nano-photosensitizer （TPP-UC（PS）） with a lanthanidedoped upconversion nanoparticle （UCNP） core coated by a photosensitizer （PS）-incorporated dense silica shell. Following irradiation with external nearinfrared laser （NIR）, TPP-UC（PS） in mitochondria caused serious mitochondrial matrix swelling for the activated upconversion-based photodynamic therapy （UC-PDT）, and the mobilization of cytochrome c （cyt c） was amplified in response to the increased mitoROS. Specifically, this heme-containing cyt c could be monitored by varying TPP-UC（PS）＇s upconversion luminescence signal （UCL）, which may facilitate the in situ detection of cyt c for apoptosis research. As a proof of concept, our designed TPP-UC（PS） may provide significant opportunities for controlling cancer cell apoptosis under NIR stimulation and for studying apoptosis using the dynamic UCL, which is influenced by local cyt c.展开更多
基金This work has been financially supported by the National Natural Science Foundation of China （Nos. 51372260, 51132009, and 81471714）, and the Shanghai Excellent Academic Leaders Program （No. 16XD1404000）. Thanks to Linlin Zhang, Heliang Yao, Qingfeng Xiao,Huaiyong Xing, Wenpei Fan, Zhaowen Cui, Li Jiang, and Jianan Liu from Shanghai Institute of Ceramics, Chinese Academy of Sciences for useful discussions.
文摘Disruption of mitochondrial reactive oxygen species （mitoROS） plays a major role in cancer cell apoptosis. Here, we designed a core/shell-structured mitochondriatargeting upconversion-based nano-photosensitizer （TPP-UC（PS）） with a lanthanidedoped upconversion nanoparticle （UCNP） core coated by a photosensitizer （PS）-incorporated dense silica shell. Following irradiation with external nearinfrared laser （NIR）, TPP-UC（PS） in mitochondria caused serious mitochondrial matrix swelling for the activated upconversion-based photodynamic therapy （UC-PDT）, and the mobilization of cytochrome c （cyt c） was amplified in response to the increased mitoROS. Specifically, this heme-containing cyt c could be monitored by varying TPP-UC（PS）＇s upconversion luminescence signal （UCL）, which may facilitate the in situ detection of cyt c for apoptosis research. As a proof of concept, our designed TPP-UC（PS） may provide significant opportunities for controlling cancer cell apoptosis under NIR stimulation and for studying apoptosis using the dynamic UCL, which is influenced by local cyt c.
