Silkworm pupa is a nourishing food with high nutritional value,but its consumption has been greatly limited given its allergenicity.Enzyme hydrolytic technique is recognized as an effective method to reduce the allerg...Silkworm pupa is a nourishing food with high nutritional value,but its consumption has been greatly limited given its allergenicity.Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein.In this study,we aimed to investigate the effect of enzymolysis on the allergenicity of silkworm pupa.Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation,which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa,and silkworm pupa were then hydrolyzed by alkaline protease.The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model,and the mice were immunized via intragastric gavage and intraperitoneal injection,respectively.The results indicated that the intraperitoneal inj ection immunization route induced more by detecting with antibodies,histamine and Th2-related cytokines.Moreover,mice treated with silkworm pupa protein peptide displayed no obvious allergic symptoms,indicating that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa.展开更多
针对农村蚕桑生产中血液型脓病危害严重的现状,利用抗病种质资源,通过杂交和回交的方法选育出抗血液型脓病的家蚕四元杂交新品种明湖春江。国家和浙江省实验室鉴定结果显示,明湖春江4龄起蚕虫蛹率、出丝率与秋丰×白玉(对照)相仿,...针对农村蚕桑生产中血液型脓病危害严重的现状,利用抗病种质资源,通过杂交和回交的方法选育出抗血液型脓病的家蚕四元杂交新品种明湖春江。国家和浙江省实验室鉴定结果显示,明湖春江4龄起蚕虫蛹率、出丝率与秋丰×白玉(对照)相仿,万蚕产茧量分别比对照提高8.78%、11.25%;解舒丝长达900 m以上,超过对照;洁净高于94分。国家和浙江省农村生产试验的张种产茧量分别比对照提高15.36%、11.06%,健蛹率与对照相仿。抗血液型脓病检测结果表明,家蚕核型多角体病毒(Bombyx mori nucleopolyhedrosis,BmNPV)对明湖春江的LC50值为3.102×10^(8)mL^(-1),对照为2.085×10^(6)mL^(-1)。新品种于2022年5月通过浙江省主要农作物品种审定委员会审定,并于2022年12月通过国家畜禽遗传资源委员会审定。该品种适合在长江流域和黄河流域秋期饲养。展开更多
基金supported by Special Project on the Integration of Industry,Education and Research of Guangdong Provine(2013B090600060)National Key R&D Program of China(2018YFC1604205)National Natural Science Foundation of China(31760431)。
文摘Silkworm pupa is a nourishing food with high nutritional value,but its consumption has been greatly limited given its allergenicity.Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein.In this study,we aimed to investigate the effect of enzymolysis on the allergenicity of silkworm pupa.Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation,which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa,and silkworm pupa were then hydrolyzed by alkaline protease.The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model,and the mice were immunized via intragastric gavage and intraperitoneal injection,respectively.The results indicated that the intraperitoneal inj ection immunization route induced more by detecting with antibodies,histamine and Th2-related cytokines.Moreover,mice treated with silkworm pupa protein peptide displayed no obvious allergic symptoms,indicating that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa.
文摘针对农村蚕桑生产中血液型脓病危害严重的现状,利用抗病种质资源,通过杂交和回交的方法选育出抗血液型脓病的家蚕四元杂交新品种明湖春江。国家和浙江省实验室鉴定结果显示,明湖春江4龄起蚕虫蛹率、出丝率与秋丰×白玉(对照)相仿,万蚕产茧量分别比对照提高8.78%、11.25%;解舒丝长达900 m以上,超过对照;洁净高于94分。国家和浙江省农村生产试验的张种产茧量分别比对照提高15.36%、11.06%,健蛹率与对照相仿。抗血液型脓病检测结果表明,家蚕核型多角体病毒(Bombyx mori nucleopolyhedrosis,BmNPV)对明湖春江的LC50值为3.102×10^(8)mL^(-1),对照为2.085×10^(6)mL^(-1)。新品种于2022年5月通过浙江省主要农作物品种审定委员会审定,并于2022年12月通过国家畜禽遗传资源委员会审定。该品种适合在长江流域和黄河流域秋期饲养。
