To investigate diagnostic accuracy of 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT in chronic coronary artery disease. Sixty-seven patients with suspecte...To investigate diagnostic accuracy of 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT in chronic coronary artery disease. Sixty-seven patients with suspected CAD were enrolled in the prospective study. All patients underwent myocardial perfusion scintigraphy in SPECT with dual isotopes of T1-201 and dipyridamole stress Tc-99m sestamibi. Rest and stress imaging protocol were performed in 2 hours by dose of 3 mCi TI-201 and 25 mCi Tc-99m sestamibi. The acquisition parameters includes LEHR collimator, energy peak of 72 and 167 keV for T1-201 and 140 keV for Tc-99m, 180-degree rotation from RAO to LPO, matrix size 64x64, and 25second/frame/64 frames. The 20-segment model of left ventricle was used in automatic quantitation software. Coronary angiography was used as gold standard. CAD was defined as 50% of lumen stenosis on coronary angiography. Rest TI-201/stress tc-99m sestamibi dual-isotope SPECT demonstrated a sensitivity of 94.59% and specificity of 70%, positive predictive value of 79.54% and negative predictive value of 91.3% in detection of coronary artery disease. Sensitivity and specificity for detecting multi-vessel coronary artery disease were 82.75% and 81.57% for the left anterior descending, 77.77% and 91.83% for left circumflex and 94.11% and 82% for right coronary artery. 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT has high sensitivity, specificity, positive predictive value and negative predictive value in detecting chronic coronary artery disease with greater than 50% stenosis assessed by coronary angiography Moreover, this imaging protocol gives high imaging quality, time-saving and convenience.展开更多
Total RNA was isolated from the hybridoma cell line (LC- 1 ), which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FRl (framework region l) and FR4 conserved reg...Total RNA was isolated from the hybridoma cell line (LC- 1 ), which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FRl (framework region l) and FR4 conserved regions of LC-1 gene, the variable regions of heavy chain (Vh) and light chain (Vl) were amplified, and the Vh and modified Vl were connected to single chain Fv (ScFv) by SOE-PCR (splice overlap extension PCR). The modified ScFv was fused with green fluorescent protein (GFP) and introduced into E. coli JM109. The fusion protein induced by lPTG (Isopropylthiogalactoside) was about 57000 on a 10% SDS-PAGE gel (10% Sds Polyacrylamide Gel Electrophoresis), and primarily manifested as inclusion bodies. The renatured protein purified by Ni-NTA Superflow resins showed ability to bind to antigen on SPC-A-l lung adenocarcinoma. In addition, the induced host cells fluoresced bright green under 395 nm wavelength, which indicated that the expected protein with dual activity was expressed in the prokaryotic system. The ScFv with GFP tag used in this research can be applied as a new reagent to detect immunological dye, and provide a feasible way to detect adenocarcinoma in a clinical setting.展开更多
In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodyna...In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodynamics coefficients of the vessel are calculated by using a 3D diffraction/radiation panel program. First- and second-order wave forces are included in the calculations. Morison equation is used to compute the drag force on line elements representing the net. Drag coefficients are determined at every time step in the simulation considering the relative normal velocity between the structural elements and the fluid flow. The dynamic response of the coupled system is analyzed for various environments and net materials. The results of the study show the effects of solidity ratio of the net and vertical positions of the cage on the overall dynamic response of the system, confirming the viability of this type of configuration for future development of offshore aquaculture in deep waters.展开更多
LVH (Left ventricular hypertrophy) is an independent risk factor for the development of heart failure, cardiac arrhythmias and stroke. A recent genome-wide association study reported the involvement of a candidate g...LVH (Left ventricular hypertrophy) is an independent risk factor for the development of heart failure, cardiac arrhythmias and stroke. A recent genome-wide association study reported the involvement of a candidate gene namely KCNBI in mechanism for development of LVH in hypertension. This study aimed to replicate the finding by investigating the genetic association of KCNBI gene among the hypertensive LVH patients from Malaysia. We genotyped a SNP (single nucleotide polymorphism) located in KCNBI namely, rs6063397 among 200 subjects consisting of61 LVH and 139 non LVH patients using Sanger sequencing method. Statistical analysis revealed no significant association between the L VH susceptibility between the allele and genotype frequencies (P = 0.2719 and 0.4768, respectively). This finding suggests that KCNBI may not playa role in LVH susceptibility in hypertensive patients in Southeast Asian populations.展开更多
Two TFs (transfer functions) are needed to analyze switching DC-DC converters in control-voltage mode: the duty-cycle to output-voltage (control to output) and the input-voltage to output-voltage (line to output...Two TFs (transfer functions) are needed to analyze switching DC-DC converters in control-voltage mode: the duty-cycle to output-voltage (control to output) and the input-voltage to output-voltage (line to output). To obtain these TFs a small-signal analysis is required. The CCM (continuous conduction mode) and the DCM (discontinuous conduction mode) analysis are different. When a circuit includes the loss resistances of the components, the number of parameters increases considerably, making manual nodal-loop circuit analysis techniques impractical to obtain the TFs. Moreover, these circuits are bilinear (non-linear) and it is necessary to linearize the equations at a DC operating-point (approximate linearization). Vorp6rian describes a PWM (pulse-width-modulated) switch model that includes all non-linear parts of the DC-DC switching converters. This model can be linearized and replaced on the switching converter schematic leading to a linear circuit. At this point it is possible to use symbolic analysis programs to obtain these TFs or to simply apply numerical values for either the Bode diagrams or the calculation of poles and zeros. Here we describe an application of Ekrem Cangeici's method on X DC-DC converter to obtain control to output and line to output TFs in CCM and DCM including loss resistances. The method presented in this paper is optimized to use in the online publishing platform OctaveRS. Also the control to output TF for PCC (peak current controlled) in CCM is obtained.展开更多
文摘To investigate diagnostic accuracy of 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT in chronic coronary artery disease. Sixty-seven patients with suspected CAD were enrolled in the prospective study. All patients underwent myocardial perfusion scintigraphy in SPECT with dual isotopes of T1-201 and dipyridamole stress Tc-99m sestamibi. Rest and stress imaging protocol were performed in 2 hours by dose of 3 mCi TI-201 and 25 mCi Tc-99m sestamibi. The acquisition parameters includes LEHR collimator, energy peak of 72 and 167 keV for T1-201 and 140 keV for Tc-99m, 180-degree rotation from RAO to LPO, matrix size 64x64, and 25second/frame/64 frames. The 20-segment model of left ventricle was used in automatic quantitation software. Coronary angiography was used as gold standard. CAD was defined as 50% of lumen stenosis on coronary angiography. Rest TI-201/stress tc-99m sestamibi dual-isotope SPECT demonstrated a sensitivity of 94.59% and specificity of 70%, positive predictive value of 79.54% and negative predictive value of 91.3% in detection of coronary artery disease. Sensitivity and specificity for detecting multi-vessel coronary artery disease were 82.75% and 81.57% for the left anterior descending, 77.77% and 91.83% for left circumflex and 94.11% and 82% for right coronary artery. 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT has high sensitivity, specificity, positive predictive value and negative predictive value in detecting chronic coronary artery disease with greater than 50% stenosis assessed by coronary angiography Moreover, this imaging protocol gives high imaging quality, time-saving and convenience.
基金Project (No. 396007) supported by the National Natural ScienceFoundation of China
文摘Total RNA was isolated from the hybridoma cell line (LC- 1 ), which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FRl (framework region l) and FR4 conserved regions of LC-1 gene, the variable regions of heavy chain (Vh) and light chain (Vl) were amplified, and the Vh and modified Vl were connected to single chain Fv (ScFv) by SOE-PCR (splice overlap extension PCR). The modified ScFv was fused with green fluorescent protein (GFP) and introduced into E. coli JM109. The fusion protein induced by lPTG (Isopropylthiogalactoside) was about 57000 on a 10% SDS-PAGE gel (10% Sds Polyacrylamide Gel Electrophoresis), and primarily manifested as inclusion bodies. The renatured protein purified by Ni-NTA Superflow resins showed ability to bind to antigen on SPC-A-l lung adenocarcinoma. In addition, the induced host cells fluoresced bright green under 395 nm wavelength, which indicated that the expected protein with dual activity was expressed in the prokaryotic system. The ScFv with GFP tag used in this research can be applied as a new reagent to detect immunological dye, and provide a feasible way to detect adenocarcinoma in a clinical setting.
基金Kampachi Farms LLC for their support to complete this work and for all the technical information provided to complete the numerical model
文摘In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodynamics coefficients of the vessel are calculated by using a 3D diffraction/radiation panel program. First- and second-order wave forces are included in the calculations. Morison equation is used to compute the drag force on line elements representing the net. Drag coefficients are determined at every time step in the simulation considering the relative normal velocity between the structural elements and the fluid flow. The dynamic response of the coupled system is analyzed for various environments and net materials. The results of the study show the effects of solidity ratio of the net and vertical positions of the cage on the overall dynamic response of the system, confirming the viability of this type of configuration for future development of offshore aquaculture in deep waters.
文摘LVH (Left ventricular hypertrophy) is an independent risk factor for the development of heart failure, cardiac arrhythmias and stroke. A recent genome-wide association study reported the involvement of a candidate gene namely KCNBI in mechanism for development of LVH in hypertension. This study aimed to replicate the finding by investigating the genetic association of KCNBI gene among the hypertensive LVH patients from Malaysia. We genotyped a SNP (single nucleotide polymorphism) located in KCNBI namely, rs6063397 among 200 subjects consisting of61 LVH and 139 non LVH patients using Sanger sequencing method. Statistical analysis revealed no significant association between the L VH susceptibility between the allele and genotype frequencies (P = 0.2719 and 0.4768, respectively). This finding suggests that KCNBI may not playa role in LVH susceptibility in hypertensive patients in Southeast Asian populations.
文摘Two TFs (transfer functions) are needed to analyze switching DC-DC converters in control-voltage mode: the duty-cycle to output-voltage (control to output) and the input-voltage to output-voltage (line to output). To obtain these TFs a small-signal analysis is required. The CCM (continuous conduction mode) and the DCM (discontinuous conduction mode) analysis are different. When a circuit includes the loss resistances of the components, the number of parameters increases considerably, making manual nodal-loop circuit analysis techniques impractical to obtain the TFs. Moreover, these circuits are bilinear (non-linear) and it is necessary to linearize the equations at a DC operating-point (approximate linearization). Vorp6rian describes a PWM (pulse-width-modulated) switch model that includes all non-linear parts of the DC-DC switching converters. This model can be linearized and replaced on the switching converter schematic leading to a linear circuit. At this point it is possible to use symbolic analysis programs to obtain these TFs or to simply apply numerical values for either the Bode diagrams or the calculation of poles and zeros. Here we describe an application of Ekrem Cangeici's method on X DC-DC converter to obtain control to output and line to output TFs in CCM and DCM including loss resistances. The method presented in this paper is optimized to use in the online publishing platform OctaveRS. Also the control to output TF for PCC (peak current controlled) in CCM is obtained.