Rest Thallium-201/Stress Technetium-99m Sestamibi Dual-Isotope Myocardial Perfusion Single-Photon Emission Computed Tomography in Detecting of Chronic Coronary Artery Disease

To investigate diagnostic accuracy of 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT in chronic coronary artery disease. Sixty-seven patients with suspected CAD were enrolled in the prospective study. All patients underwent myocardial perfusion scintigraphy in SPECT with dual isotopes of T1-201 and dipyridamole stress Tc-99m sestamibi. Rest and stress imaging protocol were performed in 2 hours by dose of 3 mCi TI-201 and 25 mCi Tc-99m sestamibi. The acquisition parameters includes LEHR collimator, energy peak of 72 and 167 keV for T1-201 and 140 keV for Tc-99m, 180-degree rotation from RAO to LPO, matrix size 64x64, and 25second/frame/64 frames. The 20-segment model of left ventricle was used in automatic quantitation software. Coronary angiography was used as gold standard. CAD was defined as 50% of lumen stenosis on coronary angiography. Rest TI-201/stress tc-99m sestamibi dual-isotope SPECT demonstrated a sensitivity of 94.59% and specificity of 70%, positive predictive value of 79.54% and negative predictive value of 91.3% in detection of coronary artery disease. Sensitivity and specificity for detecting multi-vessel coronary artery disease were 82.75% and 81.57% for the left anterior descending, 77.77% and 91.83% for left circumflex and 94.11% and 82% for right coronary artery. 2-hour protocol of rest thallium-201/stress technetium-99m sestamibi dual-isotope myocardial perfusion SPECT has high sensitivity, specificity, positive predictive value and negative predictive value in detecting chronic coronary artery disease with greater than 50% stenosis assessed by coronary angiography Moreover, this imaging protocol gives high imaging quality, time-saving and convenience.

Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

Total RNA was isolated from the hybridoma cell line （LC- 1 ）, which secretes anti-lung adenocarcinoma monoclonal antibody, and was transferred into cDNA. Based on the FRl （framework region l） and FR4 conserved regions of LC-1 gene, the variable regions of heavy chain （Vh） and light chain （Vl） were amplified, and the Vh and modified Vl were connected to single chain Fv （ScFv） by SOE-PCR （splice overlap extension PCR）. The modified ScFv was fused with green fluorescent protein （GFP） and introduced into E. coli JM109. The fusion protein induced by lPTG （Isopropylthiogalactoside） was about 57000 on a 10% SDS-PAGE gel （10% Sds Polyacrylamide Gel Electrophoresis）, and primarily manifested as inclusion bodies. The renatured protein purified by Ni-NTA Superflow resins showed ability to bind to antigen on SPC-A-l lung adenocarcinoma. In addition, the induced host cells fluoresced bright green under 395 nm wavelength, which indicated that the expected protein with dual activity was expressed in the prokaryotic system. The ScFv with GFP tag used in this research can be applied as a new reagent to detect immunological dye, and provide a feasible way to detect adenocarcinoma in a clinical setting.

Dynamic Analysis for the Global Performance of An SPM-Feeder-Cage System Under Waves and Currents

In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodynamics coefficients of the vessel are calculated by using a 3D diffraction/radiation panel program. First- and second-order wave forces are included in the calculations. Morison equation is used to compute the drag force on line elements representing the net. Drag coefficients are determined at every time step in the simulation considering the relative normal velocity between the structural elements and the fluid flow. The dynamic response of the coupled system is analyzed for various environments and net materials. The results of the study show the effects of solidity ratio of the net and vertical positions of the cage on the overall dynamic response of the system, confirming the viability of this type of configuration for future development of offshore aquaculture in deep waters.

Genetic Association Study of KCNB1 Gene with the Susceptibility of Hypertension Related LVH （Left Ventricular Hypertrophy） Patients in Malaysia

LVH （Left ventricular hypertrophy） is an independent risk factor for the development of heart failure, cardiac arrhythmias and stroke. A recent genome-wide association study reported the involvement of a candidate gene namely KCNBI in mechanism for development of LVH in hypertension. This study aimed to replicate the finding by investigating the genetic association of KCNBI gene among the hypertensive LVH patients from Malaysia. We genotyped a SNP （single nucleotide polymorphism） located in KCNBI namely, rs6063397 among 200 subjects consisting of61 LVH and 139 non LVH patients using Sanger sequencing method. Statistical analysis revealed no significant association between the L VH susceptibility between the allele and genotype frequencies （P = 0.2719 and 0.4768, respectively）. This finding suggests that KCNBI may not playa role in LVH susceptibility in hypertensive patients in Southeast Asian populations.

A Method to Obtain Complex Transfer Functions of Four Order DC-DC Converters

Two TFs （transfer functions） are needed to analyze switching DC-DC converters in control-voltage mode： the duty-cycle to output-voltage （control to output） and the input-voltage to output-voltage （line to output）. To obtain these TFs a small-signal analysis is required. The CCM （continuous conduction mode） and the DCM （discontinuous conduction mode） analysis are different. When a circuit includes the loss resistances of the components, the number of parameters increases considerably, making manual nodal-loop circuit analysis techniques impractical to obtain the TFs. Moreover, these circuits are bilinear （non-linear） and it is necessary to linearize the equations at a DC operating-point （approximate linearization）. Vorp6rian describes a PWM （pulse-width-modulated） switch model that includes all non-linear parts of the DC-DC switching converters. This model can be linearized and replaced on the switching converter schematic leading to a linear circuit. At this point it is possible to use symbolic analysis programs to obtain these TFs or to simply apply numerical values for either the Bode diagrams or the calculation of poles and zeros. Here we describe an application of Ekrem Cangeici＇s method on X DC-DC converter to obtain control to output and line to output TFs in CCM and DCM including loss resistances. The method presented in this paper is optimized to use in the online publishing platform OctaveRS. Also the control to output TF for PCC （peak current controlled） in CCM is obtained.

20世纪初青岛与上海的轮船航路——以“信利号”航运活动为中心的考察

德国1898～1914年占领山东的胶州湾,青岛逐渐发展成近代化城市。德占时期青岛的建设对东亚的轮船航运产生了重要影响,青岛也因此发展成山东海运的重要窗口。根据1909～1910年《青岛官报》的记载,德占时期,上海轮船Singlee号曾经频繁从青岛入港。该船属于中国商业轮船公司,中文名"信利号",专门从事上海和青岛之间的轮船运输,其航行的最大目的是购买胶济铁路沿线出产的煤炭。这一时期,也正是德国建好胶济铁路、大量"开发"沿线丰富煤矿资源的时期。从信利号的频繁航行可以看出,山东是上海重要的煤炭供给地,20世纪初期上海和青岛间的轮船航路是两地煤炭运输的干线航路。

新凉水塔风机振动问题的治理

针对凉水塔风机振动较大，提出了从电机开始逐级单试，解决国产凉水塔风机振动的一种行之有效的方法。

应用聚合酶链反应-单链构象多态性技术分析结节性硬化症基因突变

目的 检测结节性硬化症基因外显子突变。方法 应用聚合酶链反应 单链构象多态性 (PCR SSCP)技术分析 2 8例结节性硬化症患者TSC1和TSC2基因外显子突变情况。结果 2 8例中有 4例发生TSC1基因突变 ,其中包括 1个无义突变、2个错义突变和 1个移码突变 ;有 13例患者发生TSC2基因突变 ,其中包括 2个无义突变、2个移码突变、1个缺失和 8个错义突变。有 2例患者在TSC1及TSC2基因上均发生突变 ,另有 2例患者在TSC2核苷位 1960上出现同样突变。TSC1突变患者和TSC2突变患者之间临床表现无明显差异。结论 突变广泛分布于TSC1及TSC2基因各个外显子上 ,没有集聚于某个外显子 。