Effects of Er-Zhi-Wan on Microarchitecture and Regulation of Wnt/β-catenin Signaling Pathway in Alveolar Bone of Ovariectomized Rats

Recent studies have shown that Er-Zhi-Wan（EZW）, a traditional Chinese medicine consisting of Herba Ecliptae（HE） and Fructus Ligustri Lucidi（FLL）, had a definite antiosteoporotic effect on osteoporotic femur, but its effect on osteoporosis of alveolar bone remains unknown. In the present study, we investigated the effects of Er-Zhi-Wan（EZW） on the microarchitecture and the regulation of Wnt/β-catenin signaling pathway in the alveolar bone of ovariectomized rats. Thirty Sprague-Dawley rats were randomly divided into three groups： sham operation group（sham, n=10）, ovariectomy（OVX） group（n=10）, and OVX with EZW treatment group（EZW group, n=10）. From one week after ovariectomy, EZW（100 mg/mL） or vehicle（distilled water） was fed（1 mL/100 g） once per day for 12 weeks until the sacrifice of the rats. The body weights were measured weekly. After sacrifice, the sera and mandible were collected and routinely prepared for the measurement of alveolar trabecular microarchitecture, serum levels of E2, bone-specific alkaline phosphatase（BALP） and tartrate-resistant acid phosphatase 5b（TRAP5b）, as well as mandibular mRNA expression of Wnt/β-catenin signaling pathway molecules wnt3a, low-density lipoprotein receptor-related protein 5（LRP5）, β-catenin and dickkopf homolog 1（DKK1）. The results showed that EZW treatment significantly prevented the body weight gain, degradation of alveolar trabecular microarchitecture and alveolar bone loss in the OVX rats. Furthermore, we observed that EZW could increase the serum levels of E2 and BALP, and decrease levels of serum TRAP5b in EZW group compared with vehicle group. In addition, RT-PCR results revealed that EZW upregulated the expression levels of wnt3a, LRP5 and β-catenin, and reduced the expression of DKK1 in OVX rats. Taken together, our results suggested that EZW may have potential anti-osteoporotic effects on osteoporotic alveolar bone by stimulating Wnt/LRP5/β-catenin signaling pathway.

网络著作权纠纷交互式审理的构建与适用--以广州互联网法院ZHI系统实践为对象

交互式审理模式是一种创新的在线审理模式,实现了从“异地同时”向“异地异时”的延伸。最高人民法院《关于人民法院在线办理案件若干问题的规定(征求意见稿)》中明确了此种审理模式。其在形式上近似于“在线书面审理模式”或“斯图加特审理模式”,但在价值追求上及适用方式上有所不同,对于司法的仪式感及审判公开原则中直接、口头、对席原则等造成的冲击,可通过扩张民事诉讼程序适用范围、限定技术的应用范围与条件等措施在一定程度上予以化解。交互式审理模式以技术为支撑,以“简案快审,高效解纷”为目标,是在繁简分流的改革试点中作出的有益探索,在提高诉讼信息沟通有效性、优化民事诉讼效益、提高数据利用效率等方面具有实践合理性。本文以广州互联网法院交互式审理案件的运用实例为分析样本,评价其功效,并对该模式在夯实技术基础、拓宽应用场景、推广模式适用等方面的发展前景予以展望。

Simultaneous quantification of five major active components in capsules of the traditional Chinese medicine 'Shu-Jin-Zhi-Tong' by high performance liquid chromatography

A simple and reliable high performance liquid chromatography （HPLC） method has been developed for the simultaneous quantification of five major bioactive components in ＇Shu-Jin- Zhi-Tong＇ capsules （SJZTC）, for the purposes of quality control of this commonly prescribed traditional Chinese medicine. Under the optimum conditions, excellent separation was achieved, and the assay was fully validated in terms of linearity, precision, repeatability, stability and accuracy. The validated method was applied successfully to the determination of the five compounds in SJZTC samples from different production batches. The HPLC method can be used as a valid analytical method to evaluate the intrinsic quality of SJZTC.

Plant Regeneration from In Vitro Cultured Hypocotyl Explants of Euonymus japonicus Cu zhi

Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemented with varied concentration of different plant growth-regulators, e.g., α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA） in combination with 6-benzylaminopurine （6-BA） and kinetin. The study showed that shoots could be directly regenerated from hypocotyl explants without the intervening callus phase; MS medium was more suitable for adventitious shoots regeneration. The ability of hypocotyls segments to produce shoots varied depending upon their position on the seedlings. The highest regeneration rate was obtained with hypocotyl segments near to the cotyledon cultured on MS basal medium supplemented with 1.5 mg L^-1 6-BA and 0.05 mg L^-1 NAA （63.64%）. The regenerated shoots were readily elongated on the same medium as used for multiplication and rooted on half-strength MS basal medium supplemented with 1.0 mg L^-1 IBA and 100 mg L^-1 activated carbon. After being transferred to greenhouse conditions, 96% of the plantlets were successfully acclimatized. This regeneration system is applied for genetic transformation now.

EFFECTS OF BU GU ZHI(PSORALEA CORYLIFOLIA L) AND BAI ZHI(DAHURIAN ANGELICA ROOT) EXTRACTS ON MELANOCYTE ADHESION AND MIGRATION

Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.

资治通鉴与政府统计研究

由北宋史学家司马光编撰的《资治通鉴》包含丰富的统计数据,涵盖户籍人口、赋税徭役、军情战报、农业生产等诸多方面,是研究我国古代政府统计演变历程极为宝贵的史料基础。为总结历史经验,汲取历史智慧,本文基于对《资治通鉴》中统计活动、统计数据、统计思想和统计方法的细致钩沉与深入解读,聚焦典型案例,突出数据治理,从政府统计工作组织开展情况、政府统计的主要内容,以及防范和惩治统计造假等三个部分对五代及以前时期我国政府统计的状况加以刻画与总结。研究发现:我国古代政府统计起步时间早,渐趋成熟和稳定;统计工作历来备受重视,日益成为国家治理的有效工具和相关决策的重要基础;统计造假问题时有发生,防范和惩处力度较大;统计数据的收集利用程度与经济社会状况高度相关;统计与其他领域深度融合,专门的统计问题研究较为欠缺。

Screening of TLC Identification of Forsythin in Jin Weng Zhi Li Granule

[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.

Improvement of TLC Identification of Matrine in Jin Weng Zhi Li Granule

[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.

Pharmacognosy Identification of Yi Nationality Crude Herb "Te-zhi-pa"

[Objcetives]To study on the original plant,medicinal materials and microscopic identification of Yi Nationality crude herb Te-zhipa of Ageratum conyzoides L.,and provide experimental data for identification and application. [Methods] The original plant identification,character identification,microscopic structure identification,powder identification were adopted in theresearch.[Results] The characteristics of Yi Nationality crude herb Te-zhi-pa were obvious in the original plant,crude herband microscopic characteristics. [Conclusions]The result was provided some reference for the formulation of quality standards and original plant identification for Yi Nationality crude herb " Te-zhi-pa",and provided the basis for the further research.

Discussion on the abolishment and recovery of corporal punishment in Xingfa Zhi of JinShu

Xingfa Zhi of JinShu recorded the field of ancient Chinese law live debate on major issues in detail,providing a vivid example in understanding the development of ancient Chinese law.These issues included the abolition of revenge,the Amnesty Penalty,the stability of law,the evolution of law,and the Abolishment and recovery of the corporal punishment.This article is only discussed on the related issues of the Abolishment and recovery of the corporal punishment.

两册清代北四阁《四库全书》书叶纸张的材料分析研究

本研究以北京大学图书馆藏文渊阁和文津阁2种《四库全书》抄本纸张为研究纸样,综合分析了纸张的组成成分。结果表明,2种纸样的定量相近,厚度和紧度有一定差异,且纤维组成均为檀皮和稻草,纸张类别均为宣纸;2种纸样正反面的微观形貌相同,均有少量矿物颗粒散布于纤维空隙,表面元素均为C、O、Si、S和Ca,其中C、O集中分布区基本与纤维整体形状一致,而Ca、S主要分布于被填充的纤维孔隙,为矿物颗粒的主要成分;2种纸样均为单层结构,有矿物颗粒无规分布于纸张内部,且矿物颗粒的主要成分为生石膏(CaSO_(4)·2H_(2)O),另文渊阁抄本纸张中还含有少量方解石(CaCO_(3))。基于上述分析结果,初步推测文献中所记载的北四阁《四库全书》所用“金线榜纸”,一部分是含有生石膏成分的檀皮稻草混料单宣。

基于“脑-心-肾-精室”轴的用药观探析红参二至方在阳痿中的应用

基于“脑-心-肾-精室”轴的用药观探析红参二至方在阳痿中的应用。阳痿的发生是阴虚夹瘀而致宗筋失养,表现为肾虚血瘀证,参考“脑-心-肾-精室”轴的用药观,剖析应用经验方红参二至方治疗阳痿的经验和体会。并附验案1则。

梁繁荣教授“治神和脏”学术思想论治新冠后失眠经验撷英

本文从理论源流、治法、选穴处方及操作要点探析梁繁荣教授“治神和脏”学术思想在新冠后失眠的运用经验。梁繁荣教授认为新冠后失眠病因病机在于疠气扰神,神受邪扰,或疠气伤脏,脏不藏神,神失所养,致神不入舍,出现寤寐失常,或难以入寐,或寐而易醒,或寐而多梦。临证治疗以“治神和脏”为治则,选取头项部腧穴及背俞穴通督治神,募原穴或俞募穴相配调和脏腑,针刺手法强调虚实有别,补泻有度,对于阳虚失眠伴抑郁者辅以督脉火龙灸温阳开郁以增效,并附验案1则,以期为针灸治疗新冠后失眠提供参考。

《金匮要略》“支饮”病名含义考释

支饮病是《金匮要略》的重要组成部分。但对于其疾病命名含义,长期以来充满争议。后世医家或从医理角度、或从临床实践角度,对这一疾病命名含义作出训释,而基于文字学角度进行探讨者却较为罕见。通过对“支”字形字义之考证,同时结合对张仲景原文记载之分析,“支饮”之“支”当为“支撑”之义,“支饮”病名含义为“支撑于胸膈之水饮”。支撑于胸膈间之支饮,与位于人体腹部之痰饮、四肢之溢饮、胸胁之悬饮,涵盖了人体之大部,与“水在心”“水在肺”等“水在五脏”条文互为羽翼,从不同角度构建了仲景痰饮证治理论体系。

功夫只在自己——王阳明“致良知”的功夫论解读

王阳明的“致良知”是以“功夫之病”为问题意识的,其展开和建构是一种功夫论的进路。具体来讲,他认为做功夫有诸多缺点、错误,诸如“己私之病”“外驰之病”“支离之病”“忘助之病”,而救治“功夫之病”的药方是“知行合一”。不过,“知行合一”只是“权说”,不是“实说”。若“实说”,则乃“知行不分”,而“知行不分”的本体乃“良知”,故“实说”便是“致良知”。就“致良知”讲,针对诸种“功夫之病”,王阳明既讲了一般原则,又讲了四种针对“功夫之病”的具体对治功夫。在“致良知”功夫论说时,王阳明贯穿其中并凸显的是“功夫只在自己”的观念,而这一观念既赋予人以“主体性”,亦赋予人以“责任性”。

熄风化痰止眩汤联合悬灸治疗风痰上扰型后循环缺血性眩晕的临床研究

目的探讨熄风化痰止眩汤联合悬灸对风痰上扰型后循环缺血性眩晕患者的治疗效果。方法选取2020年5月-2023年5月江阴市中医院脑病科中医收治的81例风痰上扰型后循环缺血性眩晕患者作为研究对象,按随机数表法分为3组,各27例。其中中药组患者服用熄风化痰止眩汤,悬灸组患者采用悬灸治疗,联合组患者采用熄风化痰止眩汤及悬灸联合治疗。各组治疗周期为4周,比较2组患者的临床疗效、眩晕症状评分[Berg平衡表(BBS)评分、眩晕评估评分量表(DARS)]、脑血流速度[椎基底动脉(BA)、左椎动脉(LVA)和右椎动脉(RVA)]、中医症状积分。结果3组患者治疗后的各指标均优于治疗前。联合组的总有效率为92.60%(25/27),中药组为70.37%(19/27),悬灸组为74.07%(20/27),联合组的疗效明显优于中药组和悬灸组,差异有统计学意义(P<0.05)。联合组的BBS评分明显高于中药组和悬灸组,而DARS评分显著低于中药组和悬灸组(P<0.05);联合组BA、LVA、RVA明显高于中药组和悬灸组(P<0.05);中医症状积分在联合组明显低于中药组和悬灸组(P<0.05)。结论熄风化痰止眩汤联合悬灸治疗风痰上扰型后循环缺血性眩晕患者,可显著提高治疗效果,改善患者后循环血流和眩晕症状,具有明显优势。

基于徐荣谦教授的《神魂意魄志论》探讨孤独症谱系障碍的中医康复诊疗

孤独症谱系障碍(ASD)的发生涉及多个脏腑功能失调。徐荣谦教授所提出的“神魂意魄志论”可解释ASD的多种核心临床表现,通过深入分析神魂意魄志的功能,挖掘出本病的发生与“五脏神”相关,尤其与心神、肝魂、脾意、肾志的联系密切。临床中将这一“形神并治”的理论体系用来指导治疗ASD能使形气相得,获得较好疗效。

信息技术支持下的线上线下混合式教学模式探讨

以“智慧树”平台的“中国建筑史”课程为例,首先,探究了该课程在线上线下混合式教学模式下的设计和实践。具体包括课程的主要教学内容、特色与优势以及面临的问题。其次,深入探究了线上线下混合式教学模式的建设目标和建设思路,从教学资源与管理、教学设计与方法和教学活动与评价等方面进行了分析。最后,总结了线上线下混合式教学模式的特色,对教学实践成果进行了展示。希望能为相关课程教学提供参考和启示,促进信息技术在教学中的进一步应用和发展。

日常与非常:康熙《上溪源志》所见15至17世纪的徽州社会

日常生活的基本逻辑和对异常(乃至非常)事件之社会应对,是村落社会史研究着力揭示的一体两面,需要通过更多具体而微的文书个案加以展现。康熙《上溪源志》抄本是与现存的两种《新安上溪源程氏乡局记》相关的珍稀文献,虽然编者可能是希望将之编纂成该处村落的志书,但从现有体例来看,它仍然与《新安上溪源程氏乡局记》没有多少差别,仍旧是杂抄或史料长编的性质,离村落志成书还有相当长的距离。不过,也正因为它的原始性,提供了有明一代及清朝前期的生动资料,更成为我们了解明清时代婺源东北区域基层社会历史的珍贵史料。此外,该书也为我们探究从村落文书辑录到村落志编纂的动态过程提供了极佳的个案。