The emergence of multidrug-resistant bacterial pathogens forced us to consider the phage therapy as one of the possible alternative approaches to treatment. The purpose of this paper is to consider the conditions for ...The emergence of multidrug-resistant bacterial pathogens forced us to consider the phage therapy as one of the possible alternative approaches to treatment. The purpose of this paper is to consider the conditions for the safe, long-term use of phage therapy against various infections caused by Pseudomonas aeruginosa. We describe the selection of the most suitable phages, their most effective combinations and some approaches for the rapid recognition of phages unsuitable for use in therapy. The benefi ts and disadvantages of the various different approaches to the preparation of phage mixtures are considered, together with the specifi c conditions that are required for the safe application of phage therapy in general hospitals and the possibilities for the development of personalized phage therapy.展开更多
One of the approaches to the treatment of infections caused by multiply-(MDR) or extra drug- resistant (XDR) pathogenic strains may be application of bacterial viruses (bacteriophages)— phage therapy. Results of a lo...One of the approaches to the treatment of infections caused by multiply-(MDR) or extra drug- resistant (XDR) pathogenic strains may be application of bacterial viruses (bacteriophages)— phage therapy. Results of a long, but quite limited use of phage therapy in several Eastern European countries, as well as experiments on animal models in Western countries support the possibility to use phage therapy. However, given the role of phages in the evolution of bacterial pathogens, it is necessary to discuss and evaluate negative consequences of mass introduction of phage therapy, as the measures necessary for its safe use. We discuss some actions in case of transiting to world-wide use of phage therapy with purpose to prolong the active life of phage therapy and to diminish possible complications.展开更多
文摘The emergence of multidrug-resistant bacterial pathogens forced us to consider the phage therapy as one of the possible alternative approaches to treatment. The purpose of this paper is to consider the conditions for the safe, long-term use of phage therapy against various infections caused by Pseudomonas aeruginosa. We describe the selection of the most suitable phages, their most effective combinations and some approaches for the rapid recognition of phages unsuitable for use in therapy. The benefi ts and disadvantages of the various different approaches to the preparation of phage mixtures are considered, together with the specifi c conditions that are required for the safe application of phage therapy in general hospitals and the possibilities for the development of personalized phage therapy.
文摘One of the approaches to the treatment of infections caused by multiply-(MDR) or extra drug- resistant (XDR) pathogenic strains may be application of bacterial viruses (bacteriophages)— phage therapy. Results of a long, but quite limited use of phage therapy in several Eastern European countries, as well as experiments on animal models in Western countries support the possibility to use phage therapy. However, given the role of phages in the evolution of bacterial pathogens, it is necessary to discuss and evaluate negative consequences of mass introduction of phage therapy, as the measures necessary for its safe use. We discuss some actions in case of transiting to world-wide use of phage therapy with purpose to prolong the active life of phage therapy and to diminish possible complications.
文摘【目的】研究宽嗜性铜绿假单胞菌（PA ）的生物学特性，为临床控制PA感染提供思路。【方法】对5家医院下水道中分离出的PA噬菌体，采用双层琼脂噬菌斑形成法纯化后筛选出宽嗜性噬菌体，观察宽嗜性噬菌体的特征，绘制一步生长线，提取核酸行酶切分析。【结果】共分离出8株PA噬菌体，筛选出3株宽嗜性噬菌体，其中PAp-3表现出较宽的噬菌谱。电镜下PAp-3呈透明、圆形、边界清晰的菌斑；PAp-3基因组为23 kb ，当感染复数（MOI）为0．001时，其感染宿主产生子代PA噬菌体滴度最高，为6．4×10^10 PFU/mL ，感染宿主菌的潜伏期30 min ，爆发时间55 min ，裂解量100。3株宽嗜性噬菌体交叉组合对临床分离出的20株PA吞噬覆盖率为95．00％（19/20）。【结论】宽嗜性噬菌体具有较广的噬菌谱，潜伏期较短，裂解量大，交叉组合吞噬覆盖率高，可为噬菌体作为抗菌生物制剂提供依据。
