Mufangji tang ameliorates pulmonary arterial hypertension through improving vascular remodeling,inhibiting inflammatory response and oxidative stress,and inducing apoptosis

Background:Mufangji tang(MFJT)is composed of Ramulus Cinnamomi,Radix Ginseng,Cocculus orbiculatus(Linn.)DC.,and Gypsum.In clinical settings,MFJT has been effectively employed in addressing a range of respiratory disorders,notably including pulmonary arterial hypertension(PAH).However,the mechanism of action of MFJT on PAH remains unknown.Methods:In this study,a monocrotaline-induced PAH rat model was established and treated with MFJT.The therapeutic effects of MFJT on PAH rat model were evaluated.Network pharmacology was conducted to screen the possible targets for MFJT on PAH,and the molecular docking between the main active components and the core targets was carried out.The key targets identified from network pharmacology were tested.Results:Results showed significant therapeutic effects of MFJT on PAH rat model.Analysis of network pharmacology revealed several potential targets related to apoptosis,inflammation,oxidative stress,and vascular remodeling.Molecular docking showed that the key components were well docked with the core targets.Further experimental validation results that MFJT treatment induced apoptosis(downregulated Bcl-2 levels and upregulated Bax levels in lung tissue),inhibited inflammatory response and oxdative stress(decreased the levels of IL-1β,TNF-α,inducible NOS,and malondialdehyde,and increased the levels of endothelial nitric oxide synthase,nitric oxide,glutathione and superoxide dismutase),reduced the proliferation of pulmonary arterial smooth muscle cells(downregulated ET-1 andβ-catenin levels and ERK1/2 phosphorylation,increased GSK3βlevels).Conclusion:Our study revealed MFJT treatment could alleviate PAH in rats via induction of apoptosis,inhibition of inflammation and oxidative stress,and the prevention of vascular remodeling.

Therapeutic effect of San Bi Tang combined with glucosamine sulfate capsules in cold-dampness-type knee osteoarthritis

BACKGROUND Cold-dampness-type knee osteoarthritis is a common middle-aged and elderly disease,but its pathogenesis is not fully understood,and its clinical treatment has limitations.Glucosamine sulfate capsules are commonly used for treating arthritis,and San Bi Tang is a classic formula of traditional Chinese medicine(TCM)that has the effects of warming yang,dispelling dampness,relaxing muscles,and activating collaterals.This research hypothesized that the combination of modified San Bi Tang and glucosamine sulfate capsules could enhance the clinical efficacy of treating cold-dampness-type knee osteoarthritis through complementary effects.AIM To analyze the clinical efficacy of San Bi Tang combined with glucosamine sulfate capsules when treating cold-dampness-type knee osteoarthritis.METHODS A total of 110 patients with cold-dampness-type knee osteoarthritis were selected as research subjects and randomly divided into a control group and an experimental group of 55 cases each.The control group received only treatment with glucosamine sulfate capsules,while the experimental group received additional treatment with modified San Bi Tang for a duration of 5 wk.The patients’knee joint functions,liver and kidney function indicators,adverse reactions,and vital signs were evaluated and analyzed using SPSS 26.0 software.RESULTS Before treatment,the two groups’genders,ages,and scores were not significantly different,indicating comparability.Both groups’scores improved after treatment,which could indicate pain and knee joint function improvement,but the test group had better scores.The TCM-specific symptoms and the clinical efficacy of the treatment in the test group were higher.Before and after treatment,there were no abnormalities in the patients’liver and kidney function indicators.CONCLUSION The combination of modified San Bi Tang and glucosamine sulfate capsules is superior to treatment with sulfated glucosamine alone and has high safety.

Gaze and Oppositional Gaze in Mr. Tang’s Girls

The gaze is a means of power that can flow through family relationships.It has intense regulatory overtones.In her short story Mr.Tang’s Girls,Malaysian-Chinese Anglophone diasporic writer Shirley Geok-lin Lim shows the conflict between Eastern and Western cultures and the resistance of women under the oppression of patriarchy through the portrayal of the father,Ah Kong,and the eldest daughter,Kim Li.Based on Foucault’s discipline gaze and Hooks’oppositional gaze,this article analyzes the power operation in the story from the following three aspects:the male gaze,discipline and self-discipline,and the oppositional gaze.Kim Li’s subjective image of breaking free from male ownership control has been presented.It suggests the difficulties encountered by female groups in their rebellion against the male gaze and their pursuit of freedom and emancipation.It also reflects the tragedy of awakening women’s destinies under the dual oppression of patriarchy and the East-Westen cultural conflict.

Effects of Xiaoxianxiong Tang on biological behavior in lung cancer cell line A549

Obiective:To investigate the effects of Xiaoxianxiong Tang on the proliferation,invasion,apoptosis,and other cell biological behaviors of non-small cell lung cancer A549 cells.Methods:Human lung adenocarcinoma A549 cells were cultured in vitro,and the IC50 concentration and effective time of administration of Xiaoxianxiong Tang were determined by the CCK-8 assay to detect the inhibitory effect of Xiaoxianxiong Tang on A549 cells proliferation.The effect of the Xiaoxianxiong Tang on apoptosis was determined by flow cytometry;the apoptosis-related protein was detected via Western blot;the metastasis-related protein mRNA was detected by RT-PCR.Results:Xiaoxianxiong Tang significantly inhibited the proliferation viability,the invasive ability,and the clonogenic ability of A549 cells compared with the control group(P<0.001).Moreover,Xiaoxianxiong Tang significantly promoted the apoptosis of A549 cells(P<0.001).Xiaoxianxiong Tang significantly up-regulated Bax and down-regulated Bcl2 expression in A549 cells compared with the control group(P<0.01).The mRNA expression of MMP2 and MMP9 was significantly down-regulated by Xiaoxianxiong Tang compared with the control group(P<0.05).Conclusion:Xiaoxianxiong Tang has the effect of regulating the biological behavior of A549 cells,and Xiaoxianxiong Tang significantly inhibites the proliferation viability,colony formation,and invasion ability of lung cancer A549 cells.

Study on the mechanism of action of Lizhong Tang in ameliorating non-alcoholic fatty liver disease by regulating mitochondrial autophagy

Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index.Liver pathology was observed,while serum transaminase and blood lipid levels were measured.The protein expression levels of PINK1,Parkin,and LC-3II in rat liver were detected using Western Blot analysis.Results:Compared with the control group,the NAFLD rats exhibited a significant increase in body weight,liver wet weight,liver index,transaminase levels,and blood lipid levels.The expression levels of PINK1,Parkin,and LC3-II protein were significantly decreased(P<0.01).Following intervention with Lizhong Tang,rats in each herbal treatment group displayed a decrease in body weight,liver wet weight,liver index,se-rum transaminase,and blood lipid levels.The expression levels of PINK1,Parkin,and LC-3II rebounded(P<0.05),with the high-dose group demonstrating the most pronounced effects(P<0.01).Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure,swollen hepatocytes,disordered arrangement,and a multi-tude of varying-sized lipid vacuoles within the cytoplasm.Conversely,rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology,with the high-dose group showing the most notable enhancement.Conclusion:Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.

Clinical efficacy of external treatment with Zi Cao Yang He Tang combined with hormone therapy for granulomatous mastitis and its effect on cellular pyroptosis proteins

Objective:To observe the clinical effect of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in the treatment of granulomatous lobular mastitis in the mass stage and the effect on cellular pyroptosis-related proteins.Methods:Forty patients with granulomatous lobular mastitis were randomly divided into 20 cases in the observation group and 20 cases in the control group.Both groups were given dexamethasone ointment for treatment,and the observation group was treated with external application of Zi Cao Yang He Tang on the basis of the control group.Breast lump vol-ume changes,breast pain and pus symptoms,pathology and histology and immunohistochemistry,cellular death-related indicators expression were compared between the two groups.Results:After treatment,the difference in mass volume of the observation group was significantly greater than that of the control group(16.7±21.5 in the control group and 32.9±27.4 in the experimental group)and the difference between the two groups was statistically significant(P<0.05);the objective remission rate of the patients in the observation group(ORR=85%)was significantly higher than that of the treatment group(ORR=50%,P<0.05).In terms of pus formation rate and pain level,the breast pain score of the observation group was significantly lower than that of the control group after two weeks or three weeks of treatment(P<0.05);the pus formation rate of the observation group was 30%,and the pus formation rate of the control group was 20%,and the difference was not statistically significant(P>0.05).The immunohistochemical expression levels of caspase-1 and GSDMD in the observation group were significantly lower than those in the control group(P<0.05);and in terms of the expression of cellular pyroptosis-related indexes,the expression of GSDMD,cas-pase-1,and NLRP3 in the observation group was significantly lower compared with that in the control group(P<0.05).Conclusion:The efficacy of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in treating granulomatous lobular mastitis in the mass stage is remarkable,which can effectively reduce breast pain,reduce the size of the mass,de-crease the expression of cellular pyroptosisrelated indexes,and have a good anti-inflammatory effect.

A Study on the“Social Behavior”of Zen Poetry Culture:A Case Study of the Sui and Tang Dynasties

During the Sui and Tang dynasties,Buddhism and Zen poetry underwent great development.Zen poetry is diverse in form,strong in personality,and high in achievement.Among the types of Zen poetry are poems,lyrics,and prose,some of which have social attributes closely related to the development of Chinese Buddhism.An analysis reveals that the viewpoints and information conveyed in these poems,lyrics,and prose are positively correlated with the attitudes of the emperors towards Buddhism.When the attitude of the emperor towards Buddhism reached its peak of positivity during the Tang dynasty,there was a change towards a negative correlation with the viewpoints expressed in poetry.

Contemporary Inheritance of the Legend of ZHANG Zhongjing's Practicing Medicine in the Clinic

From the perspective of narratology, this paper explores the contemporary inheritance of the medical legacy of Zhang Zhongjing's practice medicine in the clinic. Its inheritance mode includes: the enrichment of language and characters and the narratio of romance, the spatial reductive narratio of cultural landscape, the body expressive narratio of ritual behavior. This makes the legend more concerned with the real society, enhances the regional cultural image, develops the local economy, and confidences to the practices role of carrying forward the response and mission of practicing medicine to save people. Compared with the previous academic circle, which previous focus on the textual research of the dispute over its historical authentication, or focus on the retrospective study of its historical origin, this paper is based on the research perspective of historical inheritance, which is constructive to the living inheritance and protection of legends.

Investigation of the mechanism of action of Sanwu Huangqin Tang in the treatment of restless leg syndrome based on network pharmacology,molecular docking technology and molecular dynamics simulation

Background:Through the use of network pharmacology and molecular docking approaches,this study will examine the pharmacological effects of Sanwu Huangqin Tang on restless legs syndrome in order to better understand the mechanism of action of Traditional Chinese medicine(TCM)on RLS.Method:Utilise the TCMSP database to collect and select the drug components of Sanwu Huangqin Tang,and the Uniprot database to identify pertinent targets;RLS-related disease targets were obtained from GeneCards,DrugBank,and OMIM databases;and STRING and Cytoscape 3.9.1 software were used to generate an interaction network.KEGG pathway analysis and GO enrichment analysis were performed utilizing the DAVID database.Use Autodock for analyzing the relationships between targets and core components.Result:By using a network pharmacology approach,83 active ingredients and 50 drug-disease intersecting targets were derived for Sanwu Huangqin Tang.The molecular docking results showed that the drug components had strong affinity with the average target targets.Conclusions:This study provides new insights into the mechanism through which Traditional Chinese Medicine(TCM)treats Restless Legs Syndrome(RLS).Furthermore,it lays the foundation for additional research into the mechanism of treatment for RLS through the intervention that addresses various targets and pathways using the active ingredients identified by Sanwu Huangqin Tang.

Exploration of the mechanism of action of Xiayuxue Tang against hepatic fibrosis based on GEO data mining,network pharmacology and molecular docking technology

Objective:To study the mechanism of action of Xiayuxue Tang in treating hepatic fibrosis by combining GEO data mining,network pharmacology,and molecular docking technology,and provide new research directions for the treatment of hepatic fibrosis.Method:Utilizing multiple databases,we aim to identify the relevant targets of various components in Xiayuxue Tang and their associations with hepatic fibrosis.After pinpointing the key targets through interaction analysis,we will construct both the compound-target network and the protein interaction network for Xiayuxue Tang.Conclusively,we will conduct GO and KEGG enrichment analyses on these key targets,followed by molecular docking verification.Result:Through mining the GEO database,171 related targets were identified.When combined with other databases,a total of 2,343 hepatic fibrosis-related targets were obtained.Xiayuxue Tang comprises 82 related components,which include 26 active components from rhubarb,1 from ground beetle worm,46 from peach kernels,with a total of 314 predicted targets.The GO enrichment analysis revealed 748 biological processes,32 cellular components,and 73 molecular functions,while the KEGG enrichment analysis identified 222 pathways.Molecular docking verification confirmed that effective compounds can bind stably to key proteins,exhibiting strong binding activity.This underscores the potential efficacy of Xiayuxue Tang in addressing hepatic fibrosis.Conclusion:Xiayuxue Tang exerts regulatory effects on hepatic fibrosis through different targets and pathways,suggesting that the herbal compound has the characteristics of multiple pathways and targets.

从天地会出发的哥老会——民国时期哥老会起源的文献解读

民国时期哥老会研究的重要论著大概可以划分为两种,第一种是索隐派,就是从传说和神话故事后面去寻找隐藏的历史;第二种是史料派,也就是从档案乃至其他文献中间去寻找蛛丝马迹。其实两者都指向了同一个方向,即洪门早期的这些传说和神话并不是完全没有历史资料可以佐证。实际上利用民国时期的亲历者和研究者所提供的资料和研究,从哥老会的革命叙事、民族主义叙事、会党成员的自我形象塑造、哥老会叙事的索引派和史料派等若干方面,可以进一步地理解哥老会的起源问题。这些故事的讲述,证明了在哥老会的故事中,有着大量霍布斯鲍姆概念中的“传统的发明”,但是历史显然成为他们身份认同和维系紧密关系的纽带。就这样,他们讲述的故事有多少依据固然重要,但是他们为什么要那样讲述自己的历史,却更是需要我们去认真探索的问题。

异代同调:论沈亚之传奇的骚体文学特征

以《异梦录》《秦梦记》《湘中怨解》作品为代表,唐代沈亚之的传奇文集中表现出与屈原楚辞异代而同调的典型风格,即骚体文学特征。沈亚之吸收了“骚体”独特的兮字文体范式、抒情的浪漫自然、情志的悲怨凄美等特征,与时人作品异趣。沈亚之传奇的骚体文学特征表现在以悲为美的审美内涵、浪漫自由的艺术形式以及凄艳混融的艺术风格。沈亚之创作出的具有鲜明骚体文学特征的传奇故事,亦可称之为“楚调小说”。传奇独特的骚体文学特征,使得沈亚之作品在众多传奇中独树一帜,成为一代之奇。

六味地黄汤对老化模型大鼠视网膜组织铁死亡通路的影响

目的:观察六味地黄汤对老化模型大鼠视网膜组织中铁蛋白、溶质载体家族7成员11(SLC7A11)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶4(GPX4)表达水平的影响。方法:将45只SD大鼠随机分成空白组、模型组、中药组,各15只。空白组腹腔注射生理盐水,模型组、中药组腹腔注射D-半乳糖[500 mg/(kg·d)],同时,中药组灌服六味地黄汤[6.75 g/(kg·d)],空白组、模型组灌服等体积生理盐水,连续8 wk。检测各组大鼠视网膜组织中铁蛋白、SLC7A11、GSH、GPX4的表达水平。结果:模型组较空白组大鼠视网膜组织中铁蛋白表达增高(P<0.05),GSH、SLC7A11、GPX4表达降低(P<0.05)。中药组较模型组大鼠视网膜组织中GSH、SLC7A11、GPX4表达增高(P<0.05)。结论:六味地黄汤可能通过调控铁死亡途径发挥延缓视网膜老化的作用。

唐朝初期中原与吐蕃佛教交融依存语境解读

吐蕃与唐朝因中原佛教结缘,而双方的交融依存发端于唐朝重启了儒家孟子性善论仁道思想,摒弃了过去天赋神权论的儒家思想。儒学扬弃之后在唐朝初期强调“仁”道,唐朝因“仁”和合吐蕃,佛教的中国化也在唐朝初期有了新的面目:“提倡众生平等”。儒学的“仁”转化为佛学的“众生平等”理念,形成了吐蕃与中原的基本交往范式,即以佛教为抓手,而以借鉴唐朝的基本思想为内容的事实。这也说明佛教在唐朝真正开始中原化了,而佛教在吐蕃还远未开始吐蕃化,文成公主入藏意味着吐蕃与中原启动了交往交流交融的加速模式。

羌族文化生态保护区“禹的传说”和中华文明认同的活态表达

禹作为中华文明的文化符号之一,对于中华民族的凝聚具有重要意义。禹的传说及相关禹迹遍布各地。作为非物质文化遗产的“禹的传说”在羌族文化生态保护区有较好的活态性,并以三重形态表达出来:一是汉语系统的民间传说、大禹祭祀和三官信仰;二是羌语系统的颂神禹、禹步、大禹萨朗;三是基于民间传说的禹迹。更为重要的是,岷江上游“禹的传说”的三种表达形态是叠合的,具有多语言、多形态的传承方式。三种叠合形态的一体性,说明其主导的核心价值体系是相同的,是羌族文化生态保护区的各族人民对中华文明认同的表述。

基于网络药理学探讨百合知母汤抗焦虑与失眠的分子机制

目的:应用网络药理学方法探讨百合知母汤抗焦虑与失眠的分子机制。方法:基于中医药整合药理学研究平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)V2.0对百合知母汤化学成分信息进行靶标预测,将焦虑与失眠相关疾病靶标信息进行蛋白-蛋白互作网络(protein-protein interactions,PPI)构建;富集分析百合知母汤干预焦虑、失眠的关键靶标;构建可视化的“中药方剂-中药材-化学成分-核心靶标-关键通路-疾病关键病理环节”多层次网络关联图。结果:百合知母汤有效成分主要为皂苷、黄酮、挥发油、有机酸等,百合知母汤作用潜在靶标共128个,其中与药物相关靶标14个,与疾病相关靶标114个,药物与疾病共有靶标2个,分别为NR3C1和GABRA1。百合知母汤抗焦虑与失眠主要作用于GABRA1、NFKB1、AR、SMC1A、PIK3CA、NR3C1、DRD2等基因或蛋白靶标中,涉及氨基丁酸、氯离子跨膜运输、突触后膜电位、γ-氨基丁酸的突触传递、神经元凋亡过程负调控及信号转导效应等通路。结论:百合知母汤抗焦虑与失眠的分子作用机制可能与干预GABRA1、NFKB1、DRD2等基因或蛋白表达,调控氨基丁酸、氯离子跨膜运输等通路有关。

唐朝“大一统”的空间叙事——基于《新唐书·地理志》的研究

“大一统”是中国学术界的重要议题,但对唐朝“大一统”的讨论,现有研究主要是从思想阐释层面展开,从空间叙事角度表达唐朝“大一统”历史形态的研究不多。《新唐书·地理志》对唐朝疆域范围、交通道路、户数人口、政区沿革等方面的详细记载为我们解释唐朝“大一统”提供了空间维度的观察路径与文献基础。唐朝“郡县天下-羁縻府州-文化边疆”的空间结构不仅改变了秦汉以来的“大一统”空间叙事,而且在具体实践上,唐朝所形成的“大一统”是沟通中央与地方、维系中心与边疆、涵盖中国境内与域外世界的整体性叙事。正是基于“大一统”的空间叙事,使得中国疆域具有了超越具体朝代的稳定性,成为现代中国疆域叙事体系的重要内容之一。

通俗文言小说总集编纂与宋代话本体传奇的文类特征

宋代话本体传奇的形成和兴盛,与通俗文言小说总集编纂直接相关。《青琐高议》《云斋广录》《绿窗新话》和《醉翁谈录》的主要功能之一是为说话人提供蓝本,所收《张浩》《苏小卿》《隋炀帝海山记》《温泉记》等传奇文本,与唐人传奇、宋人笔记路数迥异,而与话本格调相近。据此考察话本体传奇的文类特征,可以发现:从小说内容来看,放诞不检的“才子”“佳人”上演了一幕幕欢场大戏,与唐人辞章化传奇的唯美情调迥然不同;从文本构成来看,大量穿插诗词,以“雅的这样俗”的方式体现了浓郁的民间说唱风味;从艺术手段来看,直接描写人物心理,打破了此前文言作品的叙事惯例。宋代话本体传奇是传奇小说的一种新的文本类型。

后唐明宗朝北面行营与北部边防

北面行营的设置,是唐明宗措置军事的一项重要措施。在平定叛乱时,北面行营的主导力量是唐明宗亲信与元随以及中央的禁军。行营统帅的设置,体现了分权与制衡的原则。北面行营运行体现了唐明宗时期中央权力的发展,以及战时状态下唐明宗对于军事的掌控意愿。在战争结束后,北面行营成为后唐措置北边防御的重要机构。行营制衡的原则也运用在边防体系的构建之上,并与唐明宗的对外政策互为照映。在行营之内,唐明宗通过任命来自不同政治势力的行营统帅,派遣禁军出戍的方式,来实现行营权力的互相制衡。在后唐北部边防体系中,唐明宗设置北面行营署理河北边防,以期与负责河东边防的河东节度使实现军事上的互相制衡。但在北面行营的实际运行中,由于唐末帝时期中央激烈的政治变动以及契丹突然的军事介入,其军事上的平衡作用逐步丧失。北面行营反而成为赵德钧拥兵谋夺天下的资本。北面行营的运行反映了唐宋时期边防体制演进中对军事权力分割与制衡的趋势。

基于网络药理学方法、分子对接技术及动物实验探讨参威骨痹汤治疗骨质疏松症的作用机制

目的:探讨参威骨痹汤治疗骨质疏松症(osteoporosis,OP)的作用机制。方法:①网络药理学研究。采用超高效液相色谱-质谱法鉴定参威骨痹汤中的化合物,采用网络药理学方法初步筛选参威骨痹汤治疗OP的关键活性成分和靶点。②分子对接验证。采用分子对接技术验证网络药理学研究确定的参威骨痹汤治疗OP的关键活性成分与靶点的结合效果,从中筛选结合效果最好的组合。③动物实验验证。将60只雌性C57BL/6JNifdc小鼠随机等分为5组,模型组和参威骨痹汤低、中、高剂量组小鼠通过双侧卵巢切除手术进行OP造模,假手术组小鼠仅切取卵巢周围等体积的脂肪组织。参威骨痹汤低、中、高剂量组小鼠分别按照14.95 g·kg^(-1)·d^(-1)、29.9 g·kg^(-1)·d^(-1)、59.8 g·kg^(-1)·d^(-1)以参威骨痹汤药液灌胃,假手术组和模型组小鼠以等体积生理盐水灌胃。药物干预8周后,取股骨进行Micro-CT检查和组织病理学观察(HE染色和抗酒石酸酸性磷酸酶染色),采用实时定量PCR技术测定胫骨组织中根据网络药理学和分子对接技术确定的关键靶点基因的mRNA表达水平。结果:①网络药理学研究结果。网络药理学研究结果显示,SRC、促分裂原活化的蛋白激酶3(mitogen-activated protein kinase 3,MAPK3)、MAPK1、磷脂酰肌醇3-激酶调节亚基1(phosphatidylinositol-3-kinase regulatory subunit 1,PI3KR1)、信号转导及转录活化因子3为参威骨痹汤治疗OP的关键靶点,五味子丙素、10-姜酚、对羟基苯甲酸丁酯、倍半萜内酯和香豆雌酚为参威骨痹汤治疗OP的关键活性成分。②分子对接验证结果。分子对接结果显示,参威骨痹汤治疗OP关键活性成分与关键靶点的结合能均小于-5 kJ·mol^(-1),表明结合效果良好,其中靶点SRC、MAPK3、PI3KR1与活性成分五味子丙素、10-姜酚、对羟基苯甲酸丁酯的结合效果更好。③动物实验验证结果。Micro-CT检查及组织病理学观察结果显示,参威骨痹汤能改善OP模型小鼠的骨质疏松程度,其中中剂量参威骨痹汤效果更好。实时定量PCR测定结果显示,参威骨痹汤中剂量组胫骨组织中SRC mRNA和MAPK3 mRNA表达水平低于模型组,PI3KR1 mRNA表达水平与模型组比较差异无统计学意义。结论:参威骨痹汤治疗OP的机制可能是通过五味子丙素、10-姜酚、对羟基苯甲酸丁酯等成分调控SRC、PI3KR1、MAPK3的表达来调节骨代谢。