Objective To investigate effects of acupuncture combined with Fluoxetine on behavior and regulatory T cell （Treg） proportion in mice of chronic depression. Methods Sixty Balb/c mice were randomly divided into a norm...Objective To investigate effects of acupuncture combined with Fluoxetine on behavior and regulatory T cell （Treg） proportion in mice of chronic depression. Methods Sixty Balb/c mice were randomly divided into a normal control group （group A, n=10） and depression model group （n=50）. Fifty Balb/c mice were irritated with a variety of chronic unpredictable moderate stress stimulations for 21 days to prepare the mouse depression model. After successfully modeling, 50 depression model mice were randomly divided into 5 groups, a model group （group B）, a fluoxetine group （group C）, an acupuncture group （group D） and a combined acupuncture and medicine group （group E） and a sham acupuncture group （group F）, 10 mice in each group. The mice in the group C were intragastrically administrated with Fluoxetine once （20 mg/kg）; for the group D, electroacupuncture （EA） was given at ＂Baihui＂ （百会 GV 20） and ＂Shenting＂ （神庭 GV 24） and for the group F, EA was given at non-acupoint of the buttock for 2 min; for the group E, acupuncture combined with Fluoxetine was used with the same acupoints, methods and medicine as those in the group D and C. The treatment was given once each day, for 21 consecutive days. After treatment the open-field behavior （retention period at the central square, horizontal movement,vertical movement, etc.）, sucrose preferences, body mass, food intake and other behaviors were observed, and the proportion of regulatory T cells （CD4^＋ CD2^＋5 Foxp3^＋ Treg cells） was analyzed with flow cytometry. Results Compared with the normal control group, the open-field behavior showed depression-like changes, and humoral consumption, sucrose preferences, body mass and food intake significantly decreased in the mice of the depression model group. After treatment, compared with the group B, the behaviors of the mice in the group E, C and D were improved in varying degrees; compared with other groups, the group E had more obvious effect on vertical movement; acupuncture combined with Fluoxetine could down-regulate the high-expressed Treg cells. Conclusion Acupuncture combined with Fluoxetine can improve depression behaviors of the mice of depression in the early stage. The mechanism possibly is related with regulating Treg cells which improve the immunosuppressive status of depression.展开更多
Qianjinba is derived from the dried root of a legume vine and is known for its plant polysaccharide,which has demonstrated anti-inflammatory,analgesic,and neuroprotective properties.Despite these known effects,its imm...Qianjinba is derived from the dried root of a legume vine and is known for its plant polysaccharide,which has demonstrated anti-inflammatory,analgesic,and neuroprotective properties.Despite these known effects,its immunomodulatory potential remains underexplored.This study aimed to investigate the immunoregulatory effects of Qianjinba polysaccharide(QJBDT)in a murine model of rheumatoid arthritis(RA)and elucidate its mechanism of action in inhibiting the disease.To assess the immunomodulatory effects,RAW264.7 macrophage cells were stimulated with bacterial lipopolysaccharide(LPS)and subsequently treated with varying concentrations of QJBDT or total glucoside of paeonic acid(TGP).Cell proliferation and the expression levels of mitogen-activated protein kinase p38 and nuclear factor NF-κB proteins were evaluated.Following the successful modeling of RA in mice,different doses of QJBDT and TGP were administered via intragastric administration once daily for 21 d.Mice were divided into normal,model,QJBDT low-,medium-,and high-dose groups,along with a positive control group(TGP group),each comprising 10 mice.Organ coefficients were calculated,immune indexes in blood cells were determined,and alterations in T helper cell 17(Th17)and regulatory T cells(Treg)were analyzed using flow cytometry.Compared to the normal group,cell proliferation rates significantly increased across all groups(P<0.05).Elevated expressions of p38 and NF-κB proteins were observed in the model group,QJBDT low-and medium-dose groups(P<0.05).Various systemic immune inflammation indexes,including systemic immune inflammation index(SII),total inflammation systemic index(AISI),and systemic inflammation response index(SIRI),platelet-to-lymphocyte ratio(PLR),neutrophil-to-lymphocyte ratio(NLR),monocyte-to-lymphocyte ratio(MLR),showed increments(P<0.05).Additionally,body weight and organ coefficients of the thymus,spleen,and liver decreased in both the model and low-dose groups(P<0.05).Moreover,Th17 levels increased while Treg levels decreased across all groups(P<0.05),resulting in a heightened Th17 to Treg ratio in the model and low-dose groups(P<0.05).Notably,the QJBDT medium-dose,high-dose,and positive control groups demonstrated significant inhibitory effects on cell proliferation compared to the model group(P<0.05),along with reduced expression levels of p38 and NF-κB(P<0.05).Furthermore,various immune indicators from routine blood tests exhibited different degrees of decrease(P<0.05),while immune indices displayed increases(P<0.05).The low-,medium-,and high-dose groups of QJBDT,as well as the positive control group,showed decreased Th17 levels,elevated Treg levels,and diminished Th17 to Treg ratios(P<0.05).Additionally,there was no statistically significant difference in efficacy between the QJBDT high-dose and TGP groups(P>0.05).In conclusion,this study demonstrated that QJBDT exerted potent immunomodulatory effects on RA in mice in a dose-dependent manner.Its mechanism of action might involve the inhibition of NF-κB activation by suppressing the p38 MAPK signaling pathway.展开更多
基金Supported by Provincial College and University Natural Science Key Basic Research Project of Education Beurou, Jiangsu Province: 08 KJA 360001Jiangsu Province Qinglan "Qinglan Engineering"
文摘Objective To investigate effects of acupuncture combined with Fluoxetine on behavior and regulatory T cell （Treg） proportion in mice of chronic depression. Methods Sixty Balb/c mice were randomly divided into a normal control group （group A, n=10） and depression model group （n=50）. Fifty Balb/c mice were irritated with a variety of chronic unpredictable moderate stress stimulations for 21 days to prepare the mouse depression model. After successfully modeling, 50 depression model mice were randomly divided into 5 groups, a model group （group B）, a fluoxetine group （group C）, an acupuncture group （group D） and a combined acupuncture and medicine group （group E） and a sham acupuncture group （group F）, 10 mice in each group. The mice in the group C were intragastrically administrated with Fluoxetine once （20 mg/kg）; for the group D, electroacupuncture （EA） was given at ＂Baihui＂ （百会 GV 20） and ＂Shenting＂ （神庭 GV 24） and for the group F, EA was given at non-acupoint of the buttock for 2 min; for the group E, acupuncture combined with Fluoxetine was used with the same acupoints, methods and medicine as those in the group D and C. The treatment was given once each day, for 21 consecutive days. After treatment the open-field behavior （retention period at the central square, horizontal movement,vertical movement, etc.）, sucrose preferences, body mass, food intake and other behaviors were observed, and the proportion of regulatory T cells （CD4^＋ CD2^＋5 Foxp3^＋ Treg cells） was analyzed with flow cytometry. Results Compared with the normal control group, the open-field behavior showed depression-like changes, and humoral consumption, sucrose preferences, body mass and food intake significantly decreased in the mice of the depression model group. After treatment, compared with the group B, the behaviors of the mice in the group E, C and D were improved in varying degrees; compared with other groups, the group E had more obvious effect on vertical movement; acupuncture combined with Fluoxetine could down-regulate the high-expressed Treg cells. Conclusion Acupuncture combined with Fluoxetine can improve depression behaviors of the mice of depression in the early stage. The mechanism possibly is related with regulating Treg cells which improve the immunosuppressive status of depression.
基金Shandong Provincial Key Project of TCM Science and Technology(Grant No.2021Z051)Shandong Medical and Health Science and Technology Development Program(Grant No.202102040972)the Binzhou Medical College Student Innovation and Entrepreneurship Training Program(Grant No.X202210440354)。
文摘Qianjinba is derived from the dried root of a legume vine and is known for its plant polysaccharide,which has demonstrated anti-inflammatory,analgesic,and neuroprotective properties.Despite these known effects,its immunomodulatory potential remains underexplored.This study aimed to investigate the immunoregulatory effects of Qianjinba polysaccharide(QJBDT)in a murine model of rheumatoid arthritis(RA)and elucidate its mechanism of action in inhibiting the disease.To assess the immunomodulatory effects,RAW264.7 macrophage cells were stimulated with bacterial lipopolysaccharide(LPS)and subsequently treated with varying concentrations of QJBDT or total glucoside of paeonic acid(TGP).Cell proliferation and the expression levels of mitogen-activated protein kinase p38 and nuclear factor NF-κB proteins were evaluated.Following the successful modeling of RA in mice,different doses of QJBDT and TGP were administered via intragastric administration once daily for 21 d.Mice were divided into normal,model,QJBDT low-,medium-,and high-dose groups,along with a positive control group(TGP group),each comprising 10 mice.Organ coefficients were calculated,immune indexes in blood cells were determined,and alterations in T helper cell 17(Th17)and regulatory T cells(Treg)were analyzed using flow cytometry.Compared to the normal group,cell proliferation rates significantly increased across all groups(P<0.05).Elevated expressions of p38 and NF-κB proteins were observed in the model group,QJBDT low-and medium-dose groups(P<0.05).Various systemic immune inflammation indexes,including systemic immune inflammation index(SII),total inflammation systemic index(AISI),and systemic inflammation response index(SIRI),platelet-to-lymphocyte ratio(PLR),neutrophil-to-lymphocyte ratio(NLR),monocyte-to-lymphocyte ratio(MLR),showed increments(P<0.05).Additionally,body weight and organ coefficients of the thymus,spleen,and liver decreased in both the model and low-dose groups(P<0.05).Moreover,Th17 levels increased while Treg levels decreased across all groups(P<0.05),resulting in a heightened Th17 to Treg ratio in the model and low-dose groups(P<0.05).Notably,the QJBDT medium-dose,high-dose,and positive control groups demonstrated significant inhibitory effects on cell proliferation compared to the model group(P<0.05),along with reduced expression levels of p38 and NF-κB(P<0.05).Furthermore,various immune indicators from routine blood tests exhibited different degrees of decrease(P<0.05),while immune indices displayed increases(P<0.05).The low-,medium-,and high-dose groups of QJBDT,as well as the positive control group,showed decreased Th17 levels,elevated Treg levels,and diminished Th17 to Treg ratios(P<0.05).Additionally,there was no statistically significant difference in efficacy between the QJBDT high-dose and TGP groups(P>0.05).In conclusion,this study demonstrated that QJBDT exerted potent immunomodulatory effects on RA in mice in a dose-dependent manner.Its mechanism of action might involve the inhibition of NF-κB activation by suppressing the p38 MAPK signaling pathway.
