AIM: To clarify whether -238G/A polymorphism of tumor necrosis factor-a (TNF-a) gene promoter region was associated with outcomes of hepatitis B virus (HBV) infection in Han population of northern China, and to analyz...AIM: To clarify whether -238G/A polymorphism of tumor necrosis factor-a (TNF-a) gene promoter region was associated with outcomes of hepatitis B virus (HBV) infection in Han population of northern China, and to analyze the geneenvironment interaction between -238G/A polymorphism and cigarette smoking or alcohol consumption. METHODS: A case-control study was conducted to analyze the association of TNF-a gene promoter polymorphism with HBV infection outcomes. A total of 207 patients with chronic hepatitis B (HB) and 148 cases of self-limited HBV infection from Ditan Hospital and Shunyi District Hospital in Beijing, respectively were recruited. History of smoking and alcohol drinking was inquired by a questionnaire. The -238G/A polymorphism of TNF-a gene promoter was genotyped by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP). RESULTS: The frequencies of GG and GA genotypes were 98.07% and 1.93% in chronic HB patients and 93.24% and 6.76% in self-limited HBV infection individuals, respectively (X^2=5.30, P=-0.02). The frequency of G allele was significantly higher in patients with chronic HB that in individuals with self-limited HBV infection (99.03% vs 96.62%, X^2=5.20, P=0.02). Only modestly increased risk of onset of chronic HB was found in smokers (OR=1.40, 95% CI： 0.87-2.28, P=0.14) and drinkers (OR=-1.26, 95%CI： 0.78-2.05, P=-0.32). There was a positive interaction between genotype GG and cigarette smoking with an interaction index (Ⅱ) of 2.95, or alcohol consumption with an Ⅱ of 1.64. CONCLUSION: The -238G/A polymorphism of TNF-a gene promoter region is independently associated with different outcomes of HBV infection.展开更多
目的探讨广西汉族人群不同中医证型缺血性脑卒中易感性与肿瘤坏死因子-α(TNF-α)G238A和G308A位点基因多态性的关系。方法采用Sequenom Mass ARRAY中通量分型方法对255例风痰瘀阻证以及182例气虚血瘀证缺血性脑卒中患者的G238A和G308A...目的探讨广西汉族人群不同中医证型缺血性脑卒中易感性与肿瘤坏死因子-α(TNF-α)G238A和G308A位点基因多态性的关系。方法采用Sequenom Mass ARRAY中通量分型方法对255例风痰瘀阻证以及182例气虚血瘀证缺血性脑卒中患者的G238A和G308A位点进行基因分型检测,并对其基因型及基因频率进行比较。结果风痰瘀阻证以及气虚血瘀证患者与对照者间的TNF-α(G238A,G308A)基因型及基因频率分布的差异无统计学意义(P>0.05)。结论TNF-α基因G238A和G308A多态性与缺血性中风之风痰瘀阻证及气虚血瘀证的易感性可能无关。展开更多
基金Supported by the Research Funit for the Doctoral Training Program from the Ministry of Education,No.2000002340 and Beijing Municipal Government Commission for Science & Technology,No.H020920020590
文摘AIM: To clarify whether -238G/A polymorphism of tumor necrosis factor-a (TNF-a) gene promoter region was associated with outcomes of hepatitis B virus (HBV) infection in Han population of northern China, and to analyze the geneenvironment interaction between -238G/A polymorphism and cigarette smoking or alcohol consumption. METHODS: A case-control study was conducted to analyze the association of TNF-a gene promoter polymorphism with HBV infection outcomes. A total of 207 patients with chronic hepatitis B (HB) and 148 cases of self-limited HBV infection from Ditan Hospital and Shunyi District Hospital in Beijing, respectively were recruited. History of smoking and alcohol drinking was inquired by a questionnaire. The -238G/A polymorphism of TNF-a gene promoter was genotyped by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP). RESULTS: The frequencies of GG and GA genotypes were 98.07% and 1.93% in chronic HB patients and 93.24% and 6.76% in self-limited HBV infection individuals, respectively (X^2=5.30, P=-0.02). The frequency of G allele was significantly higher in patients with chronic HB that in individuals with self-limited HBV infection (99.03% vs 96.62%, X^2=5.20, P=0.02). Only modestly increased risk of onset of chronic HB was found in smokers (OR=1.40, 95% CI： 0.87-2.28, P=0.14) and drinkers (OR=-1.26, 95%CI： 0.78-2.05, P=-0.32). There was a positive interaction between genotype GG and cigarette smoking with an interaction index (Ⅱ) of 2.95, or alcohol consumption with an Ⅱ of 1.64. CONCLUSION: The -238G/A polymorphism of TNF-a gene promoter region is independently associated with different outcomes of HBV infection.
文摘目的探讨广西汉族人群不同中医证型缺血性脑卒中易感性与肿瘤坏死因子-α(TNF-α)G238A和G308A位点基因多态性的关系。方法采用Sequenom Mass ARRAY中通量分型方法对255例风痰瘀阻证以及182例气虚血瘀证缺血性脑卒中患者的G238A和G308A位点进行基因分型检测,并对其基因型及基因频率进行比较。结果风痰瘀阻证以及气虚血瘀证患者与对照者间的TNF-α(G238A,G308A)基因型及基因频率分布的差异无统计学意义(P>0.05)。结论TNF-α基因G238A和G308A多态性与缺血性中风之风痰瘀阻证及气虚血瘀证的易感性可能无关。
