目的：研究女性体内有机氯农药pp’-DDE残留与乳腺癌发病风险的关联及关联强度,为女性乳腺癌的预防及治疗提供数据支持。方法：检索中国生物医学文献期刊数据库、中国知网、万方数据库、Pub Med、Web of Science等数据库中从2000年至201...目的：研究女性体内有机氯农药pp’-DDE残留与乳腺癌发病风险的关联及关联强度,为女性乳腺癌的预防及治疗提供数据支持。方法：检索中国生物医学文献期刊数据库、中国知网、万方数据库、Pub Med、Web of Science等数据库中从2000年至2015年有关有机氯农药暴露与乳腺癌发病风险的病例对照研究,筛选出符合纳入标准的合格文献,应用Revman 5.0和Stata软件进行分析。结果：共检索获得2 039篇文章,共13篇符合纳入标准。Meta分析结果显示：在血清和脂肪组织中,pp’-DDE平均含量病例组要高于对照组;血清来源的研究pp’-DDE暴露水平与乳腺癌患病风险趋势OR=1.10,95%CI：1.01~1.20,脂肪组织来源的研究pp’-DDE暴露水平与乳腺癌患病风险趋势OR=1.17,95%CI：1.11~1.23;异质性检验P=0.24,I2=24%;亚组分析三个亚组P值均〈0.05,均有统计学意义。结论：有机氯农药pp’-DDE残留可能是乳腺癌的一个危险因素,有效预防和控制有机氯农药的摄入和接触可能有助于减轻患乳腺癌的风险性。展开更多
Although 1,1-dichloro-2,2-bis（p-chlorophenyl）-ethylene （p,p′-DDE）, the major and most persistent metabolite of dichlorodiphenyltrichloroethane （DDT）, was continually detected in wild fishes that showed abnormal...Although 1,1-dichloro-2,2-bis（p-chlorophenyl）-ethylene （p,p′-DDE）, the major and most persistent metabolite of dichlorodiphenyltrichloroethane （DDT）, was continually detected in wild fishes that showed abnormal gonad development such as intersex, little is known about the impact of p,p′-DDE exposure on gonad development in fishes. To survey the effects of p,p′-DDE on gonadal development and gene expressions, male juvenile （20-d post hatch） Japanese medaka （Oryzias latipes） was exposed to 1, 5, 20, and 100 μg/L p,p′-DDE for two months. Increased hepatosomatic index （HSI） and decreased gonadosomatic index （GSI） were found in the p,p′-DDE-treated groups. Intersex was found in 100 μg/L p,p′-DDE exposure group, as well as 100 ng/L 17α-ethynylestradiol （EE2） group. By quantitative real-time RT-PCR, it was found that gene expressions of vitellogenins （VTG-1, VTG-2）, choriogenins （CHG-H, CHG-L）, and estrogen receptor α （ER-α） in the liver of the fish were significantly up-regulated by p,p′-DDE exposure. VTG-1 and VTG- 2 were recommended as the preferred biomarker for assessing anti-androgenic p,p′-DDE because they were the highest up-regulated among the genes and showed good dose-response relationship. The up-regulated ER-α suggested that a potential synergetic effect would occur when p,p′-DDE coexists with other ER-α-binding endocrine-disrupting chemicals （EDCs）.展开更多
基金supported by the National Natural Science Foundation of China(No.40632009,20607002)the National Basic Research Program(973)of China(No.2007CB407304).
文摘Although 1,1-dichloro-2,2-bis（p-chlorophenyl）-ethylene （p,p′-DDE）, the major and most persistent metabolite of dichlorodiphenyltrichloroethane （DDT）, was continually detected in wild fishes that showed abnormal gonad development such as intersex, little is known about the impact of p,p′-DDE exposure on gonad development in fishes. To survey the effects of p,p′-DDE on gonadal development and gene expressions, male juvenile （20-d post hatch） Japanese medaka （Oryzias latipes） was exposed to 1, 5, 20, and 100 μg/L p,p′-DDE for two months. Increased hepatosomatic index （HSI） and decreased gonadosomatic index （GSI） were found in the p,p′-DDE-treated groups. Intersex was found in 100 μg/L p,p′-DDE exposure group, as well as 100 ng/L 17α-ethynylestradiol （EE2） group. By quantitative real-time RT-PCR, it was found that gene expressions of vitellogenins （VTG-1, VTG-2）, choriogenins （CHG-H, CHG-L）, and estrogen receptor α （ER-α） in the liver of the fish were significantly up-regulated by p,p′-DDE exposure. VTG-1 and VTG- 2 were recommended as the preferred biomarker for assessing anti-androgenic p,p′-DDE because they were the highest up-regulated among the genes and showed good dose-response relationship. The up-regulated ER-α suggested that a potential synergetic effect would occur when p,p′-DDE coexists with other ER-α-binding endocrine-disrupting chemicals （EDCs）.
