A rapid, straightforward, sensitive, efficient, and cost-effective reverse-phase high-performance liquid chromatographic method was employed for the simultaneous determination of Sorbitol, Sodium Lactate, and Chloride...A rapid, straightforward, sensitive, efficient, and cost-effective reverse-phase high-performance liquid chromatographic method was employed for the simultaneous determination of Sorbitol, Sodium Lactate, and Chlorides in a drug solution for infusion. Sorbitol, Sodium lactate, and Chloride are all officially recognized in the USP monograph. Assay methods are provided through various techniques, with titrations being ineffective for trace-level quantification. Alternatively, IC, AAS, and ICP-MS, though highly accurate, are costly and often unavailable to most testing facilities. When considering methods, it’s important to prioritize both quality control requirements and user-friendly techniques. A simple HPLC simultaneous method was developed for the quantification of Chlorides, Sorbitol, and Sodium Lactate with a shorter run time. The separation utilized a Shimpack SCR-102(H) ion exclusion analytical column (7.9 mm × 300 mm, 7 μm), with a flow rate of 0.6 mL per min. The column compartment temperature was maintained at 40°C, and the injection volume was set at 10 μL, with detection at 200 nm. All measurements were conducted in a 0.1% solution of phosphoric acid. The analytical curves demonstrated linearity (r > 0.9999) in the concentration range of 0.79 to 3.8 mg per mL for Sodium Lactate (SL), 0.16 to 0.79 mg per mL for Sodium Chloride (SC), and 1.5 to 7.2 mg per mL for Sorbitol. Validation of the developed method followed the guidelines of the International Conference on Harmonization (ICH Q2B) and USP. The method exhibited precision, robustness, accuracy, and selectivity. In accelerated stability testing over 6 months, no significant variations were observed in organoleptic analysis and pH. Consequently, the developed method is deemed suitable for routine quality control analyses, enabling the simultaneous determination of Sodium Lactate, Sodium Chloride, and Sorbitol in pharmaceutical formulations and infusions.展开更多
样品经正己烷第一次萃取后,氮吹至干,再由乙腈-水第二次萃取,乙腈层经改进的QuEChERS方法净化,以0.1%(V/V)甲酸水溶液-乙腈为流动相梯度洗脱,C18色谱柱分离,HPLC-MS/MS多反应监测(MRM)模式测定,基质匹配外标法定量。结果表明,在9种基质...样品经正己烷第一次萃取后,氮吹至干,再由乙腈-水第二次萃取,乙腈层经改进的QuEChERS方法净化,以0.1%(V/V)甲酸水溶液-乙腈为流动相梯度洗脱,C18色谱柱分离,HPLC-MS/MS多反应监测(MRM)模式测定,基质匹配外标法定量。结果表明,在9种基质(葡萄、梨、甘蔗、黄瓜、芹菜、土豆、大豆、玉米、大米)中,噁唑酰草胺在1.0~50.0ng/mL范围内的线性关系均较好(r>0.999),定量限在0.5μg/kg~1.0μg/kg;在1.0、5.0、10.0μg/kg3个添加水平下,平均回收率为63.9%~113.7%,相对标准偏差(relative standard deviation,RSD)为1.0%~22.2%(n=6)。该方法快速、灵敏、简便、准确,可用于多种植源性食品中噁唑酰草胺农药残留的定性和定量检测。展开更多
文摘A rapid, straightforward, sensitive, efficient, and cost-effective reverse-phase high-performance liquid chromatographic method was employed for the simultaneous determination of Sorbitol, Sodium Lactate, and Chlorides in a drug solution for infusion. Sorbitol, Sodium lactate, and Chloride are all officially recognized in the USP monograph. Assay methods are provided through various techniques, with titrations being ineffective for trace-level quantification. Alternatively, IC, AAS, and ICP-MS, though highly accurate, are costly and often unavailable to most testing facilities. When considering methods, it’s important to prioritize both quality control requirements and user-friendly techniques. A simple HPLC simultaneous method was developed for the quantification of Chlorides, Sorbitol, and Sodium Lactate with a shorter run time. The separation utilized a Shimpack SCR-102(H) ion exclusion analytical column (7.9 mm × 300 mm, 7 μm), with a flow rate of 0.6 mL per min. The column compartment temperature was maintained at 40°C, and the injection volume was set at 10 μL, with detection at 200 nm. All measurements were conducted in a 0.1% solution of phosphoric acid. The analytical curves demonstrated linearity (r > 0.9999) in the concentration range of 0.79 to 3.8 mg per mL for Sodium Lactate (SL), 0.16 to 0.79 mg per mL for Sodium Chloride (SC), and 1.5 to 7.2 mg per mL for Sorbitol. Validation of the developed method followed the guidelines of the International Conference on Harmonization (ICH Q2B) and USP. The method exhibited precision, robustness, accuracy, and selectivity. In accelerated stability testing over 6 months, no significant variations were observed in organoleptic analysis and pH. Consequently, the developed method is deemed suitable for routine quality control analyses, enabling the simultaneous determination of Sodium Lactate, Sodium Chloride, and Sorbitol in pharmaceutical formulations and infusions.
文摘样品经正己烷第一次萃取后,氮吹至干,再由乙腈-水第二次萃取,乙腈层经改进的QuEChERS方法净化,以0.1%(V/V)甲酸水溶液-乙腈为流动相梯度洗脱,C18色谱柱分离,HPLC-MS/MS多反应监测(MRM)模式测定,基质匹配外标法定量。结果表明,在9种基质(葡萄、梨、甘蔗、黄瓜、芹菜、土豆、大豆、玉米、大米)中,噁唑酰草胺在1.0~50.0ng/mL范围内的线性关系均较好(r>0.999),定量限在0.5μg/kg~1.0μg/kg;在1.0、5.0、10.0μg/kg3个添加水平下,平均回收率为63.9%~113.7%,相对标准偏差(relative standard deviation,RSD)为1.0%~22.2%(n=6)。该方法快速、灵敏、简便、准确,可用于多种植源性食品中噁唑酰草胺农药残留的定性和定量检测。