Objective.To study the change of taurine transport,and taurine transporter(TAUT)and cysteine sulfinate decarboxylase(CSD)mRNA contents in hypertension and hypertensive cardiomegaly.Methods.Taurine content was determin...Objective.To study the change of taurine transport,and taurine transporter(TAUT)and cysteine sulfinate decarboxylase(CSD)mRNA contents in hypertension and hypertensive cardiomegaly.Methods.Taurine content was determined with a amino acid analyser.Taurine uptakes were deter-mined by( 3 H)-taurine incubation.The content of TAUT and CSD mRNA levels were measured by com-petitive quantitative RT-PCR in myocardial and vascular tissues of L-N ω -nitro-arginine(L-NNA)-in-duced hypertensive rats.Results.After the treatment of rats with L-NNA for28days,myocardial and vascular taurine con-tents decreased by11%and15%(P<0.05),respectively,and plasma taurine level increased by13%(P<0.05).Myocardial and vascular Vmax of taurine uptake reduced by30%and19%(P<0.05),respec-tively.Their Km of taurine uptake increased by36%and17%(P<0.05).Myocardial and vascular TAUT mRNA content decreased by22%and19%(P<0.05),respectively,but CSD mRNA content increased by22%(P<0.05)and30%(P<0.01),respectively.Conclusions.This study suggests that there is a decreased taurine content in myocardial and vascu-lar tissues of L-NNA-induced hypertension rats.The decreased taurine content may result from the de-creased number of TAUT on the cell membrane mainly due to the down-regulation of TAUT gene and TAUT affinity caused by hypertension and hypertensive cardiomegaly.展开更多
文摘Objective.To study the change of taurine transport,and taurine transporter(TAUT)and cysteine sulfinate decarboxylase(CSD)mRNA contents in hypertension and hypertensive cardiomegaly.Methods.Taurine content was determined with a amino acid analyser.Taurine uptakes were deter-mined by( 3 H)-taurine incubation.The content of TAUT and CSD mRNA levels were measured by com-petitive quantitative RT-PCR in myocardial and vascular tissues of L-N ω -nitro-arginine(L-NNA)-in-duced hypertensive rats.Results.After the treatment of rats with L-NNA for28days,myocardial and vascular taurine con-tents decreased by11%and15%(P<0.05),respectively,and plasma taurine level increased by13%(P<0.05).Myocardial and vascular Vmax of taurine uptake reduced by30%and19%(P<0.05),respec-tively.Their Km of taurine uptake increased by36%and17%(P<0.05).Myocardial and vascular TAUT mRNA content decreased by22%and19%(P<0.05),respectively,but CSD mRNA content increased by22%(P<0.05)and30%(P<0.01),respectively.Conclusions.This study suggests that there is a decreased taurine content in myocardial and vascu-lar tissues of L-NNA-induced hypertension rats.The decreased taurine content may result from the de-creased number of TAUT on the cell membrane mainly due to the down-regulation of TAUT gene and TAUT affinity caused by hypertension and hypertensive cardiomegaly.
