Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to chara...Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.展开更多
The interaction of ternary complexes [RE(phen)(cin)_3(H_2O)](RE= Nd (III),Ce(III),Eu(III))with calf thymus DNA was investigated by electronic absorption spectroscopy, ethidium bromide(EB)-DNA quenching fluorescent spe...The interaction of ternary complexes [RE(phen)(cin)_3(H_2O)](RE= Nd (III),Ce(III),Eu(III))with calf thymus DNA was investigated by electronic absorption spectroscopy, ethidium bromide(EB)-DNA quenching fluorescent spectra and resonance light scattering. All experimental results indicate that the three complexes bind to DNA by the electrostatic mode and the intercalative mode. It is predicted that these complexes show high anticancer activity or other biologic activity.展开更多
基金financially supported by the National Natural Science Foundation of China(No.20975067)RFDP (No.20090073120039)Shanghai Educational Development Foundation(No.2008CG12)
文摘Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.
文摘The interaction of ternary complexes [RE(phen)(cin)_3(H_2O)](RE= Nd (III),Ce(III),Eu(III))with calf thymus DNA was investigated by electronic absorption spectroscopy, ethidium bromide(EB)-DNA quenching fluorescent spectra and resonance light scattering. All experimental results indicate that the three complexes bind to DNA by the electrostatic mode and the intercalative mode. It is predicted that these complexes show high anticancer activity or other biologic activity.
