Development of a target capture sequencing SNP genotyping platform for genetic analysis and genomic breeding in rapeseed

Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy.Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest.In this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named BnaPan50T.The platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic traits.The capture quality of BnaPan50T was demonstrated well in 12 typical accessions.Compared with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide application.Target capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the platform.BnaPan50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification resources.This study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.

The Impact of Music Education on Children’s Cognitive Development:From Traditional Classrooms to Modern Digital Platforms

Music education has long been debated for its influence on children’s cognitive development,particularly regarding their thinking methods and adaptability.This article synthesizes research data to examine the cognitive benefits of music instruction,including increased IQ,language proficiency,memory,and attention.Traditional face-to-face training,while personalized and socially interactive,faces limitations such as budget constraints and accessibility.Modern digital platforms offer individualized learning paths with AI-driven feedback but may lack necessary interpersonal interaction.This paper proposes a hybrid approach to music education,integrating traditional and digital methods to maximize cognitive gains.Further research is recommended to explore the implementation of these integrated learning strategies in varied educational settings.

Research on the Legal Framework of Content Regulations for Network Platforms

In the era of the Internet,various network platforms have evolved into new hubs for information dissemination.Currently,China has established a platform-centered content regulation framework,wherein platforms proactively enforce content regulations in accordance with legal censorship obligations.Additionally,platform policies and user agreements augment their authority in content regulation.The platforms can achieve cost-effective and highly efficient content regulation by leveraging their strategic advantages enabled by their own technical capabilities and extensive coverage.The platform self-regulation model,however,still faces challenges.First,accurately evaluating content remains a formidable task;second,ensuring effective platform publicity through self-regulation poses difficulties;third,users may potentially face disadvantages due to the platform’s right of self-regulation;and fourth,digital copyright owners face challenges when defending digital copyright disputes under the safe harbor rule.Therefore,it is imperative to establish,review,and revise the legal framework for content regulation of network platforms in order to enhance the efficiency of their governance systems.The formulation of the legal framework for content regulation of network platforms may encompass the following aspects:rationalizing obligations pertaining to platform content regulations,enhancing supervision over platform self-regulation,and establishing a dual-track responsibility system for digital copyright content regulation.This will ensure a harmonious balance among public interests,users’personal rights and interests,and commercial benefits through regulating the content on network platforms.

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in Coilia nasus

Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic potential.However,the species faces challenges due to significant variation in the gonadal development rate among females,resulting in inconsistent ovarian maturation times at the population level,an extended reproductive period,and limitations on fish growth rate due to ovarian prematurity.In the present study,we combined genome-wide association study(GWAS)and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms(SNPs)and candidate genes associated with population-asynchronous ovarian development in C.nasus.Genotyping of the female population based on whole-genome resequencing yielded 2120695 high-quality SNPs,39 of which were suggestively associated with ovarian development.Of note,a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified,with cpne5a determined as the causal gene of the peak.Therefore,single-marker and haplotype association analyses were performed on cpne5a,revealing four genetic markers(P<0.05)and seven haplotypes(r2>0.9)significantly associated with the phenotype.Comparative transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary,respectively,between individuals of different body sizes.Integrating the GWAS and transcriptome analysis results,this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion,extracellular matrix,angiogenesis,and gap junctions involved in population-asynchronous ovarian development.The insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of C.nasus strains exhibiting population-synchronous ovarian development in the future.

Application Analysis of Doctor-Nurse Cooperation Follow-up Model Based on the Internet Platform in Continuation Care of Patients after Urooncology Surgery

Objective:To explore the application effect of a doctor-nurse cooperation follow-up model based on an Internet platform in the continuation of care for patients after uro-oncology surgery.Methods:A convenient sampling method was used to select patients with urinary system tumors who underwent surgery in the Department of Urology in Grade III A general hospital in Shanghai from May to August 2022.Patients who underwent surgery from May to June 2022 were assigned to the control group,and those who underwent surgery from July to August 2022 were assigned to the experimental group.The control group received routine post-discharge nursing health education and telephone follow-up.On the basis of routine discharge guidance,the experimental group implemented the intervention method based on the Internet platform in continuation care.The levels of self-management efficacy,satisfaction,and incidence of unplanned readmission were compared one month after discharge between the two groups.Results:One month after discharge,the self-management efficacy of the experimental group(90.15±7.92)was significantly higher than that of the control group(79.10±7.84),and the patient satisfaction score(97.83±2.32)was significantly higher than that of the control group(90.23±2.58),with statistical significance(P<0.05).Additionally,the incidence of unplanned readmissions within one month after discharge in the experimental group(1.59%)was slightly lower than that in the control group(4.84%).Conclusion:The doctor-nurse cooperation follow-up model based on the Internet platform in continuation care can significantly improve the self-management efficiency of patients after discharge and enhance patient satisfaction,providing a new approach for discharge follow-up of urological tumor patients after surgery.

Research on Network Security Level Protection Measurement Strategy in the Context of Cloud Platforms

Platforms facilitate information exchange,streamline resources,and reduce production and management costs for companies.However,some viral information may invade and steal company resources,or lead to information leakage.For this reason,this paper discusses the standards for cybersecurity protection,examines the current state of cybersecurity management and the risks faced by cloud platforms,expands the time and space for training on cloud platforms,and provides recommendations for measuring the level of cybersecurity protection within cloud platforms in order to build a solid foundation for them.

The Role and Place of Artificial Neural Network Architectures Structural Redundancy in the Input Data Prototypes and Generalization Development

Neural Networks (NN) are the functional unit of Deep Learning and are known to mimic the behavior of the human brain to solve complex data-driven problems. Whenever we train our own neural networks, we need to take care of something called the generalization of the neural network. The performance of Artificial Neural Networks (ANN) mostly depends upon its generalization capability. In this paper, we propose an innovative approach to enhance the generalization capability of artificial neural networks (ANN) using structural redundancy. A novel perspective on handling input data prototypes and their impact on the development of generalization, which could improve to ANN architectures accuracy and reliability is described.

Rapid Prototype Development Approach for Genetic Programming

Genetic Programming (GP) is an important approach to deal with complex problem analysis and modeling, and has been applied in a wide range of areas. The development of GP involves various aspects, including design of genetic operators, evolutionary controls and implementations of heuristic strategy, evaluations and other mechanisms. When designing genetic operators, it is necessary to consider the possible limitations of encoding methods of individuals. And when selecting evolutionary control strategies, it is also necessary to balance search efficiency and diversity based on representation characteristics as well as the problem itself. More importantly, all of these matters, among others, have to be implemented through tedious coding work. Therefore, GP development is both complex and time-consuming. To overcome some of these difficulties that hinder the enhancement of GP development efficiency, we explore the feasibility of mutual assistance among GP variants, and then propose a rapid GP prototyping development method based on πGrammatical Evolution (πGE). It is demonstrated through regression analysis experiments that not only is this method beneficial for the GP developers to get rid of some tedious implementations, but also enables them to concentrate on the essence of the referred problem, such as individual representation, decoding means and evaluation. Additionally, it provides new insights into the roles of individual delineations in phenotypes and semantic research of individuals.

New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

The Development and Operation Mechanism of the Colleges’E-class Network Ideological and Political Education Platform——Based on post-95 college students’social behavior characteristics

Based on the investigation and analysis of the social behavior characteristics of post-95undergraduates, this paper puts forward the principle of sharing and building e-class platform and analyzes thedevelopment of the functions of the four platforms such as the teacher-student service system, instant interactionsystem, SNS community system and vertical segmental pushing system and the realization of the function of modularoperation. Finally, in order to ensure the stable operation of the E-class platform, four measures are put forward suchas the systematic construction of the system, the innovation of team work, the assessment of key performances and thecomprehensive guarantee, thus providing an external guarantee for the stable operation of the E-class platform.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

A Novel Verification Development Platform for Passive UHF RFID Tag

This paper introduces a novel verification development platform for the passive UHF RFID tag,which is compatible with the ISO/IEC 18000-6B standard,operating in the 915MHz ISM band. This platform efficiently reduces the design and development time and cost, and implements a fast prototype design of the passive UHF RFID tag. It includes the RFID analog front end and the tag control logic, which is implemented in an Altera ACEX FPGA. The RFID analog front end, which is fabricated using a Chartered 0.35μm two-poly four-metal CMOS process, contains a local oscillator, power on reset circuit, matching network and backscatter, rectifier, regu- lator,AM demodulator, etc. The platform achieves rapid, flexible and efficient verification and development, and can also be fit for other RFID standards after changing the tag control logic in FPGA.

Design and Implementation of an Open Network Security Management Platform

In order to manage all kinds of network security devices and software systems efficiently, and make them collaborate with each other, the model for an open network security management platform is presented. The feasibility and key implementing technology of the model are expatiated. A prototype system is implemented to validate it.

Constructing Ideas about GIS Universal Development Platform in Beijing Municipal Bureau of Agriculture

In order to solve repeat construction, waste of resources and other issues, to meet GIS application rapid customization development of different business units of the Beijing Municipal Bureau of Agriculture, and to achieve implementation agriculture GIS Applications Standard Specification for unified, to finally build agriculture GIS application, middleware technology was introduced into bureau of agriculture of Beijing City general development platform for GIS construction, and embedded object technology and seamless docking technology were made use of to accomplish quick service system between GM and sharing, to avoid duplication of large software development work, raise agriculture to GIS application development efficiency, reduce the cost of development, shorten the development cycle.

Learning and Talent Development Alignment——take Network Railway as an example

Learning and talent development(LTD)aims to enhance an organisation’s performance,the need for LTD to link to the organisation’s overall business strategy,which also be described as linkage/alignment,is more imperative than ever in this rapid changing environment.This paper aims to explore how Network Rail utilise LTD strategies to support its overall business strategies.

Floating Production Platforms and their Applications in the Development of Oil and Gas Fields in the South China Sea

This paper studies the current available options for floating production platforms in developing deepwater oil fields and the potential development models of future oil and gas exploration in the South China Sea. A detailed review of current deepwater platforms worldwide was performed through the examples of industry projects, and the pros and cons of each platform are discussed. Four types of platforms are currently used for the deepwater development: tension leg platform, Spar, semi-submersible platform, and the floating production system offloading. Among these, the TLP and Spar can be used for dry tree applications, and have gained popularity in recent years. The dry tree application enables the extension of the drilling application for fixed platforms into floating systems, and greatly reduces the cost and complexity of the subsea operation. Newly built wet tree semi-submersible production platforms for ultra deepwater are also getting their application, mainly due to the much needed payload for deepwater making the conversion of the old drilling semi-submersible platforms impossible. These platforms have been used in different fields around the world for different environments; each has its own advantages and disadvantages. There are many challenges with the successful use of these floating platforms. A lot of lessons have been learned and extensive experience accumulated through the many project applications. Key technologies are being reviewed for the successful use of floating platforms for field development, and potential future development needs are being discussed. Some of the technologies and experience of platform applications can be well used for the development of the South China Sea oil and gas field.

Application of Tension Leg Platform Combined with Other Systemsin the Development of China Deepwater Oil Fields

The development of deepwater oil fields has reached a new stage with the dramatic increase in water depth and the recent increasing demands of the economic development in the filed. The use of a Tension Leg Platform （TIP） combined with other systems, such as Floating Production Storage and Offloading （FPSO） system, Floating Production Unit （FPU） system, Tender Assisted Drilling （TAD） system, etc., has drawn the industry attention and increased significantly in the past few years. For the areas lacking of pipeline system, the use of TIP（s） combined with FPSO has been chosen to efficiently develop the deepwater fields. The TIP with a Tender Assisted Drilling system significantly reduces the payload of the platform and reduces the investment in the TIP system substantially. This opens the door for many new deepwater field developments to use the tension leg platform. The advantage of the TIP combined with a TAD system is more significant when several TIPs are used for the continuous development of the field. One of the applications for the TIP with a tender assisted drilling system can be in the development of an offshore marginal field. Owing to the increase of water depth, the conventional fixed platform model for the exploration of those fields becomes uneconomical. It also would be too expensive to use a large TIP structure for those marginal fields due to the large amount of initial investment. The TIP system with tender assisted drilling can be used to develop those fields economically. There are many marginal fields in China offshore, especially in shelf areas. The application of this field developing model, combined with the existing field developing experience in China, will open the door for many marginal field developments. This paper will review the application of the combined TIP system through some examples of completed/ongoing projects, and major technical issues encountered in those practices. The potential application of this technology in China deepwater development will be discussed in the end.

Development of the High-performance Synchronous Permanent Magnet Planar Motor and Its Key Technologies

永磁同步平面电机具有直接驱动、出力密度高、低热耗、高精度等特点,在现代精密、超精密加工装备,特别是在极紫外光刻机中具有广阔的应用前景。根据永磁阵列和线圈阵列的结构形式将永磁同步平面电机划分为组合式、半组合式、绕组层叠式和对称式永磁同步平面电机4类,并介绍了各种类型平面电机的特点,总结了永磁同步平面电机关键技术的发展现状,针对目前永磁同步平面电机存在的不足,讨论了未来永磁同步平面电机发展的趋势与方向。

基于OneNET云平台的智慧消防远程监控系统的设计

针对传统消防监控系统存在开发成本高、误警率高、实时监控不便的问题,提出一种基于物联网云平台的智慧消防远程监控系统。采用STM32单片机作为中枢控制芯片,经多传感器采集温度、湿度、烟雾、火焰等环境数据,通过窄带物联网(NB-IoT,Narrow Band Internet of Things)上传至OneNET云平台。经数据分析后以可视化方式呈现,对异常数据触发报警实时响应。通过手机APP实现数据实时监测及一键处置。经测试,监控系统报警准确率高于97.2%,数据延迟低于50 ms,表明该系统能够实现消防火警的无线远程监控,并做出快速反应,满足中小微企业和普通家庭用户的消防监控需要。