明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,...明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,主区为浇水次数,设春浇一水(W1,拔节水)和春浇两水(W2,拔节水+开花水);副区为氮肥施用量,设N0、N1、N2、N3、N4和N5(0、60、120、180、240和300 kg hm^(-2))6个水平。结果表明,施氮量0~300 kg hm^(-2)时,不同降水年型春浇一水、春浇两水小麦产量随施氮量的增加均先增加后减少,产量最高值对应的施氮量均为240 kg hm^(-2)。施氮量120~300 kg hm^(-2)时,春浇两水处理产量显著高于春浇一水处理。水氮互作对小麦单位面积收获穗数的影响最大,其次是千粒重,对穗粒数的影响最小。施氮量0~300 kg hm^(-2)时,2017-2018年度(丰水年型),春浇两水小麦湿面筋含量、沉降值、吸水率、面团稳定时间、拉伸能量、最大拉伸阻力平均值均高于春浇一水,而2018-2019、2019-2020年度(干旱年型)则相反:春浇一水高于春浇两水。不同降水年型春浇一水、春浇两水小麦湿面筋含量和沉降值随施氮量的增加先增加后减少或逐渐增加,二者最大值对应的施氮量为240 kg hm^(-2)或300 kg hm^(-2);稳定时间、拉伸能量和最大拉伸阻力随施氮量的增加均先增加后减少,施氮量240 kg hm^(-2)时达到最大值。不同降水年型强筋优质小麦师栾02-1生育期春浇两水、施氮量240 kg hm^(-2)时,籽粒产量和加工品质表现最佳。展开更多
目的阐明深海来源曲霉16-02-1的代谢产物及其抗肿瘤抗真菌活性。方法采用活性跟踪模式,利用多种色谱技术分离纯化代谢产物,结合化学反应的理化及波谱数据鉴定化合物。采用MTT法测试抗肿瘤活性,纸片法测试抗真菌活性。结果从曲霉16-02-1...目的阐明深海来源曲霉16-02-1的代谢产物及其抗肿瘤抗真菌活性。方法采用活性跟踪模式,利用多种色谱技术分离纯化代谢产物,结合化学反应的理化及波谱数据鉴定化合物。采用MTT法测试抗肿瘤活性,纸片法测试抗真菌活性。结果从曲霉16-02-1产物中分离鉴定了新曲霉酸(1)、ferrineoaspergillin(2)、(2'S)-4-甲氧基-3-(2'-甲基-3'-羟基)丙酰基-苯甲酸甲酯(3)、黄曲霉素(4)、环(反式-4-羟基-L-脯氨酸-L-亮氨酸)(5)、环(反式-4-羟基-L-脯氨酸-L-苯丙氨酸)(6)、尿嘧啶(7)和(11S)-新羟基曲霉酸(8)等8个化合物。化合物1～8对人癌细胞K562、HL-60、HeLa、BGC-823有一定抑制作用,在100μg.mL-1浓度下对K562细胞的抑制率在33.6%～43.6%之间,1和8还对白色念珠菌和土曲霉表现出较弱抑菌活性。结论首次从深海来源真菌产物中分离得到化合物1～4和8,其中1为曲霉16-02-1的主产物,发酵产率28.8mg/L。首次报道3的2'S和8的11S绝对构型、8的13 C NMR数据及其在DMSO-d6和CD3OD中的1 H NMR数据、以及8在DMSO-d6中酮式―烯醇式互变异构的NMR证据。化合物2～4和8对部分人癌细胞的抑制活性亦首次测试报道。展开更多
高光谱遥感技术已广泛应用于植被类型制图。然而,稀疏植被冠层覆盖和土壤背景影响仍然是干旱区植被类型遥感分类的主要挑战,单独利用遥感数据光谱或纹理特征难以获得可靠的分类精度和稳定性。广义正态分布优化算法(Generalized Normal D...高光谱遥感技术已广泛应用于植被类型制图。然而,稀疏植被冠层覆盖和土壤背景影响仍然是干旱区植被类型遥感分类的主要挑战,单独利用遥感数据光谱或纹理特征难以获得可靠的分类精度和稳定性。广义正态分布优化算法(Generalized Normal Distribution Optimization,GNDO)的特征优选结果在质量和稳定性方面相较传统优化算法具有优势,但目前还未应用于高光谱波段选取研究。为探索结合ZY-102D光谱与纹理特征进行干旱区植被类型遥感分类的可行性,验证GNDO方法应用于高光谱波段选取的有效性,同时探讨不同数量训练像元条件下,各特征选取方法的选择结果差异和对植被类型分类精度的影响,该研究以青海省都兰县宗加镇为例,在随机选取各分类类别不同数量训练像元(30、50、100、150、200)基础上,分别利用遗传算法(Genetic Algorithm,GA)、粒子群优化算法(Particle Swarm Optimization,PSO)、灰狼优化算法(Grey Wolf Optimization,GWO)以及GNDO算法进行高光谱波段选取并对比结果,同时利用灰度共生矩阵(Gray-Level Co-occurrence Matrix,GLCM)方法提取纹理特征,将提取的光谱特征和纹理特征组合成30组分类数据集,利用随机森林(Random Forest,RF)方法完成植被类型自动分类,对比不同分类数据集的分类精度。结果显示:蓝波段(400~450 nm)、红边波段(700~750 nm)和红波段(600~650 nm)对区分植被类型最敏感;基于光谱特征的分类数据集中,使用200个训练像元和GNDO方法进行特征优选获取的分类数据集(GNDO200)获得了最高的总体分类精度(80.44%);随着训练像元的增加,各分类数据集总体分类精度整体均呈上升趋势,不同的特征选择方法的分类精度对训练像元数量表现出不同的依赖程度;图像纹理特征的加入,明显提升了植被分类精度,将使用200个训练像元和GWO方法进行波段优选的结果与纹理特征结合的分类数据集(GWO200+TEX)获得了最高的总体分类精度(82.86%)。该研究验证了ZY1-02D国产高光谱卫星数据光谱纹理特征结合进行干旱区植被类型划分的潜力,证实了GNDO方法对高光谱波段选取的有效性,为高光谱植被类型制图中光谱、纹理特征选取提供了一种思路。展开更多
HSV-1 infection-mediated regulation of mRNA translation in host cells is a systematic and complicated process. Investigation of the details of this mechanism will facilitate understanding of biological variations in t...HSV-1 infection-mediated regulation of mRNA translation in host cells is a systematic and complicated process. Investigation of the details of this mechanism will facilitate understanding of biological variations in the viral replication process and host cells. In this study, a comparative proteomics technology platform was applied by two-dimension electrophoresis of HSV-1 infected normal human L-02 cell and control cell lysates. The observed protein spots were analyzed qualitatively and quantitatively by the PDQuest software package. A number of the different observed protein spots closely associated with cellular protein synthesis were identified by matrix-assisted laser-desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). The expression levels of the RPLP1 protein, which is required for mRNA translation, and KHSRP protein, which is involved in rapid decay of mRNA, were up-regulated, whereas the expression level of RNP H2, which is involved in positive regulation on the mRNA splicing process, was down-regulated. All of these results suggest that HSV-1 infection can influence cellular protein synthesis via modulation of cellular regulatory proteins involved in RNA splicing, translation and decay, resulting in optimisation of viral protein synthesis when cellular protein synthesis is shut off. Although there is need for further investigations regarding the detailed mechanisms of cellular protein control, our studies provide new insight into the targeting of varied virus signaling pathways involved in host cellular protein synthesis.展开更多
At 15:10 on May 6, with the command of "fire" from the Command and Control Center of the Jiuquan Satellite Launch Center, the Tianhui 1-02 satellite was launched by a LM-2D rocket into space. 680 seconds aft...At 15:10 on May 6, with the command of "fire" from the Command and Control Center of the Jiuquan Satellite Launch Center, the Tianhui 1-02 satellite was launched by a LM-2D rocket into space. 680 seconds after the take-off, the satellite separated from the rocket and entered its planned orbit.展开更多
文摘明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,主区为浇水次数,设春浇一水(W1,拔节水)和春浇两水(W2,拔节水+开花水);副区为氮肥施用量,设N0、N1、N2、N3、N4和N5(0、60、120、180、240和300 kg hm^(-2))6个水平。结果表明,施氮量0~300 kg hm^(-2)时,不同降水年型春浇一水、春浇两水小麦产量随施氮量的增加均先增加后减少,产量最高值对应的施氮量均为240 kg hm^(-2)。施氮量120~300 kg hm^(-2)时,春浇两水处理产量显著高于春浇一水处理。水氮互作对小麦单位面积收获穗数的影响最大,其次是千粒重,对穗粒数的影响最小。施氮量0~300 kg hm^(-2)时,2017-2018年度(丰水年型),春浇两水小麦湿面筋含量、沉降值、吸水率、面团稳定时间、拉伸能量、最大拉伸阻力平均值均高于春浇一水,而2018-2019、2019-2020年度(干旱年型)则相反:春浇一水高于春浇两水。不同降水年型春浇一水、春浇两水小麦湿面筋含量和沉降值随施氮量的增加先增加后减少或逐渐增加,二者最大值对应的施氮量为240 kg hm^(-2)或300 kg hm^(-2);稳定时间、拉伸能量和最大拉伸阻力随施氮量的增加均先增加后减少,施氮量240 kg hm^(-2)时达到最大值。不同降水年型强筋优质小麦师栾02-1生育期春浇两水、施氮量240 kg hm^(-2)时,籽粒产量和加工品质表现最佳。
文摘目的阐明深海来源曲霉16-02-1的代谢产物及其抗肿瘤抗真菌活性。方法采用活性跟踪模式,利用多种色谱技术分离纯化代谢产物,结合化学反应的理化及波谱数据鉴定化合物。采用MTT法测试抗肿瘤活性,纸片法测试抗真菌活性。结果从曲霉16-02-1产物中分离鉴定了新曲霉酸(1)、ferrineoaspergillin(2)、(2'S)-4-甲氧基-3-(2'-甲基-3'-羟基)丙酰基-苯甲酸甲酯(3)、黄曲霉素(4)、环(反式-4-羟基-L-脯氨酸-L-亮氨酸)(5)、环(反式-4-羟基-L-脯氨酸-L-苯丙氨酸)(6)、尿嘧啶(7)和(11S)-新羟基曲霉酸(8)等8个化合物。化合物1～8对人癌细胞K562、HL-60、HeLa、BGC-823有一定抑制作用,在100μg.mL-1浓度下对K562细胞的抑制率在33.6%～43.6%之间,1和8还对白色念珠菌和土曲霉表现出较弱抑菌活性。结论首次从深海来源真菌产物中分离得到化合物1～4和8,其中1为曲霉16-02-1的主产物,发酵产率28.8mg/L。首次报道3的2'S和8的11S绝对构型、8的13 C NMR数据及其在DMSO-d6和CD3OD中的1 H NMR数据、以及8在DMSO-d6中酮式―烯醇式互变异构的NMR证据。化合物2～4和8对部分人癌细胞的抑制活性亦首次测试报道。
文摘高光谱遥感技术已广泛应用于植被类型制图。然而,稀疏植被冠层覆盖和土壤背景影响仍然是干旱区植被类型遥感分类的主要挑战,单独利用遥感数据光谱或纹理特征难以获得可靠的分类精度和稳定性。广义正态分布优化算法(Generalized Normal Distribution Optimization,GNDO)的特征优选结果在质量和稳定性方面相较传统优化算法具有优势,但目前还未应用于高光谱波段选取研究。为探索结合ZY-102D光谱与纹理特征进行干旱区植被类型遥感分类的可行性,验证GNDO方法应用于高光谱波段选取的有效性,同时探讨不同数量训练像元条件下,各特征选取方法的选择结果差异和对植被类型分类精度的影响,该研究以青海省都兰县宗加镇为例,在随机选取各分类类别不同数量训练像元(30、50、100、150、200)基础上,分别利用遗传算法(Genetic Algorithm,GA)、粒子群优化算法(Particle Swarm Optimization,PSO)、灰狼优化算法(Grey Wolf Optimization,GWO)以及GNDO算法进行高光谱波段选取并对比结果,同时利用灰度共生矩阵(Gray-Level Co-occurrence Matrix,GLCM)方法提取纹理特征,将提取的光谱特征和纹理特征组合成30组分类数据集,利用随机森林(Random Forest,RF)方法完成植被类型自动分类,对比不同分类数据集的分类精度。结果显示:蓝波段(400~450 nm)、红边波段(700~750 nm)和红波段(600~650 nm)对区分植被类型最敏感;基于光谱特征的分类数据集中,使用200个训练像元和GNDO方法进行特征优选获取的分类数据集(GNDO200)获得了最高的总体分类精度(80.44%);随着训练像元的增加,各分类数据集总体分类精度整体均呈上升趋势,不同的特征选择方法的分类精度对训练像元数量表现出不同的依赖程度;图像纹理特征的加入,明显提升了植被分类精度,将使用200个训练像元和GWO方法进行波段优选的结果与纹理特征结合的分类数据集(GWO200+TEX)获得了最高的总体分类精度(82.86%)。该研究验证了ZY1-02D国产高光谱卫星数据光谱纹理特征结合进行干旱区植被类型划分的潜力,证实了GNDO方法对高光谱波段选取的有效性,为高光谱植被类型制图中光谱、纹理特征选取提供了一种思路。
基金National Natural Science Funds (30570081 and 30670094)
文摘HSV-1 infection-mediated regulation of mRNA translation in host cells is a systematic and complicated process. Investigation of the details of this mechanism will facilitate understanding of biological variations in the viral replication process and host cells. In this study, a comparative proteomics technology platform was applied by two-dimension electrophoresis of HSV-1 infected normal human L-02 cell and control cell lysates. The observed protein spots were analyzed qualitatively and quantitatively by the PDQuest software package. A number of the different observed protein spots closely associated with cellular protein synthesis were identified by matrix-assisted laser-desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). The expression levels of the RPLP1 protein, which is required for mRNA translation, and KHSRP protein, which is involved in rapid decay of mRNA, were up-regulated, whereas the expression level of RNP H2, which is involved in positive regulation on the mRNA splicing process, was down-regulated. All of these results suggest that HSV-1 infection can influence cellular protein synthesis via modulation of cellular regulatory proteins involved in RNA splicing, translation and decay, resulting in optimisation of viral protein synthesis when cellular protein synthesis is shut off. Although there is need for further investigations regarding the detailed mechanisms of cellular protein control, our studies provide new insight into the targeting of varied virus signaling pathways involved in host cellular protein synthesis.
文摘At 15:10 on May 6, with the command of "fire" from the Command and Control Center of the Jiuquan Satellite Launch Center, the Tianhui 1-02 satellite was launched by a LM-2D rocket into space. 680 seconds after the take-off, the satellite separated from the rocket and entered its planned orbit.
