本文在分析当前CAPP(Computer Aided Process Planning)系统普遍存在问题的基础上,提出了利用对象建模技术对CAPP系统进行分析和建模,利用基于COM的组件技术对CAPP系统进行开发,构成一个可重构CAPP开发工具.并结合实例,详细论述了利用...本文在分析当前CAPP(Computer Aided Process Planning)系统普遍存在问题的基础上,提出了利用对象建模技术对CAPP系统进行分析和建模,利用基于COM的组件技术对CAPP系统进行开发,构成一个可重构CAPP开发工具.并结合实例,详细论述了利用这两种方法对减速器类CAPP开发工具系统进行分析、设计和开发的过程.展开更多
Glucose metabolism plays a key role in thymocyte development. The mammalian target of rapamycin complex 2 (mT0RC2) is a critical regulator of cell growth and metabolism, but its role in early thymocyte development and...Glucose metabolism plays a key role in thymocyte development. The mammalian target of rapamycin complex 2 (mT0RC2) is a critical regulator of cell growth and metabolism, but its role in early thymocyte development and metabolism has not been fully studied. We show here that genetic ablation of Sinl, an essential component of mTORC2, in T lineage cells results in severely impaired thymocyte development at the CD4^- CD8^- double negative (DN) stages but not at the CD4^+ CD8^+ double positive (DP) or later stages. Notably, Sinl-deficient DN thymocytes show markedly reduced proliferation and glycolysis.Importantly, we discover that the M2 isoform of pyruvate kinase (PKM2) is a novel and crucial Sinl effector in promoting DN thymocyte development and metabolism. At the molecular level, we show that Sinl-mTORC2 controls PKM2 expression through an AKT-dependent PPAR-y nuclear translocation. Together, our study unravels a novel mTORC2-PPAR-γ-PKM2 pathway in immune-metabolic regulation of early thymocyte development.展开更多
文摘本文在分析当前CAPP(Computer Aided Process Planning)系统普遍存在问题的基础上,提出了利用对象建模技术对CAPP系统进行分析和建模,利用基于COM的组件技术对CAPP系统进行开发,构成一个可重构CAPP开发工具.并结合实例,详细论述了利用这两种方法对减速器类CAPP开发工具系统进行分析、设计和开发的过程.
基金the National Natural Science Foundation of China (31470845, 81430033, and 31670896)Shanghai Science and Technology Commission (13JC1404700)Shanghai Rising-Star Program (16QA1403300).
文摘Glucose metabolism plays a key role in thymocyte development. The mammalian target of rapamycin complex 2 (mT0RC2) is a critical regulator of cell growth and metabolism, but its role in early thymocyte development and metabolism has not been fully studied. We show here that genetic ablation of Sinl, an essential component of mTORC2, in T lineage cells results in severely impaired thymocyte development at the CD4^- CD8^- double negative (DN) stages but not at the CD4^+ CD8^+ double positive (DP) or later stages. Notably, Sinl-deficient DN thymocytes show markedly reduced proliferation and glycolysis.Importantly, we discover that the M2 isoform of pyruvate kinase (PKM2) is a novel and crucial Sinl effector in promoting DN thymocyte development and metabolism. At the molecular level, we show that Sinl-mTORC2 controls PKM2 expression through an AKT-dependent PPAR-y nuclear translocation. Together, our study unravels a novel mTORC2-PPAR-γ-PKM2 pathway in immune-metabolic regulation of early thymocyte development.
