In this papert we construct unconstrained methods for the generalized nonlinearcomplementarity problem and variational inequalities. Properties of the correspon-dent unconstrained optimization problem are studied. We ...In this papert we construct unconstrained methods for the generalized nonlinearcomplementarity problem and variational inequalities. Properties of the correspon-dent unconstrained optimization problem are studied. We apply these methods tothe subproblems in trust region method, and study their interrelationships. Nu-merical results are also presented.展开更多
Consider a discrete time dynamical system x_(k+1)=f(x_k) on a compact metric space M, wheref: M→M is a continuous map. Let h:M→R^k be a continuous output function. Suppose that all ofthe positive orbits of f are den...Consider a discrete time dynamical system x_(k+1)=f(x_k) on a compact metric space M, wheref: M→M is a continuous map. Let h:M→R^k be a continuous output function. Suppose that all ofthe positive orbits of f are dense and that the system is observable. We prove that any outputtrajectory of the system determines f and h and M up to a homeomorphism.If M is a compactAbelian topological group and f is an ergodic translation, then any output trajectory determinesthe system up to a translation and a group isomorphism of the group.展开更多
目的:构建针对人microRNA-194过表达及抑制表达的慢病毒表达载体,寻找与探讨感染人骨肉瘤细胞系SO-SP-9607和U2-os的最佳步骤和方法。方法:利用PCR方法调取相应目的基因进行酶切,经电泳回收后与目的基因进行连接,产物转化细菌感受态细胞...目的:构建针对人microRNA-194过表达及抑制表达的慢病毒表达载体,寻找与探讨感染人骨肉瘤细胞系SO-SP-9607和U2-os的最佳步骤和方法。方法:利用PCR方法调取相应目的基因进行酶切,经电泳回收后与目的基因进行连接,产物转化细菌感受态细胞,对克隆进行PCR鉴定和测序对比分析后,构建相应microRNA-194过表达及抑制表达慢病毒表达载体;在人骨肉瘤细胞系SO-SP-9607和U2-os转染及筛选过程中,根据不同阶段及浓度设定相应实验组,并设相应对照组。倒置显微镜观察转染效率,筛选情况,进行比较。结果:PCR及测序结果证实重组慢病毒表达质粒构建正确。过表达及抑制表达重组慢病毒的滴度分别为1.5×108TU/ml及4×108TU/ml。感染复数(multiply of infection,MOI)值测定,实验组及对照组转染效率无明显差异,获得MOI值及感染时间数据。通过新的综合设计,经筛选后获得转染效率满意的目的克隆细胞。结论:成功构建了microRNA-194过表达及抑制表达慢病毒表达载体,并通过新的综合考虑设计,对人骨肉瘤细胞系SO-SP-9607和U2-os进行转染和筛选后,可较快和较高效率获得满意目的细胞。展开更多
文摘In this papert we construct unconstrained methods for the generalized nonlinearcomplementarity problem and variational inequalities. Properties of the correspon-dent unconstrained optimization problem are studied. We apply these methods tothe subproblems in trust region method, and study their interrelationships. Nu-merical results are also presented.
文摘Consider a discrete time dynamical system x_(k+1)=f(x_k) on a compact metric space M, wheref: M→M is a continuous map. Let h:M→R^k be a continuous output function. Suppose that all ofthe positive orbits of f are dense and that the system is observable. We prove that any outputtrajectory of the system determines f and h and M up to a homeomorphism.If M is a compactAbelian topological group and f is an ergodic translation, then any output trajectory determinesthe system up to a translation and a group isomorphism of the group.
文摘目的:构建针对人microRNA-194过表达及抑制表达的慢病毒表达载体,寻找与探讨感染人骨肉瘤细胞系SO-SP-9607和U2-os的最佳步骤和方法。方法:利用PCR方法调取相应目的基因进行酶切,经电泳回收后与目的基因进行连接,产物转化细菌感受态细胞,对克隆进行PCR鉴定和测序对比分析后,构建相应microRNA-194过表达及抑制表达慢病毒表达载体;在人骨肉瘤细胞系SO-SP-9607和U2-os转染及筛选过程中,根据不同阶段及浓度设定相应实验组,并设相应对照组。倒置显微镜观察转染效率,筛选情况,进行比较。结果:PCR及测序结果证实重组慢病毒表达质粒构建正确。过表达及抑制表达重组慢病毒的滴度分别为1.5×108TU/ml及4×108TU/ml。感染复数(multiply of infection,MOI)值测定,实验组及对照组转染效率无明显差异,获得MOI值及感染时间数据。通过新的综合设计,经筛选后获得转染效率满意的目的克隆细胞。结论:成功构建了microRNA-194过表达及抑制表达慢病毒表达载体,并通过新的综合考虑设计,对人骨肉瘤细胞系SO-SP-9607和U2-os进行转染和筛选后,可较快和较高效率获得满意目的细胞。
