AIM: CMU-1 is a new preservation solution with a low potassium concentration as well as low viscosity that is highly effective in reducing preservation injury. The purpose of this experiment is to compare the protecti...AIM: CMU-1 is a new preservation solution with a low potassium concentration as well as low viscosity that is highly effective in reducing preservation injury. The purpose of this experiment is to compare the protective effect of CMU-1 solution with that of UW during cold preservation and normothermic reperfusion. METHODS: Wistar rats were divided into two groups according to different preservation solution: CMU-1 group and UW group. After 6, 12 and 24 h cold storage of rat liver in different preservation solutions, the isolated perfused rat liver model was applied to reperfuse the liver for 120 min normothermically (37 癈) with Krebs-Henseleit solution, meanwhile the pH value of the preservation solution was measured. The perfusate was sampled for the evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). At the end of the reperfusion, all of the bile product was collected, energy metabolic substrate and histological examination were performed. RESULTS: After preserving for 6 h, pH value of both groups did not change; after 12 h, both decreased but with no significant difference. After 24 h, pH value in UW solution group significantly decreased. The total adenine nucleotides level and AEC in liver tissue decreased with preservation time, but they were higher in CMU-1 group. And the amount of bile product after perfusion for 120 min in CMU-1 group was much more than that in UW group. However, there were no significant differences in ALT and LDH levels between two groups. Histology showed no difference. CONCLUSION: The preservation effect of CMU-1 solution is similar with that of UW solution. However, CMU-1 solution shows some advantages over UW solution in energy metabolism, preventing intracellular acidosis and bile product.展开更多
A simple and effective method has been developed to reflect the growth of bacteria CH-1via the potential of the solution.The results indicate that during the bacterial cultivation,the biomass increases and the potenti...A simple and effective method has been developed to reflect the growth of bacteria CH-1via the potential of the solution.The results indicate that during the bacterial cultivation,the biomass increases and the potential of the solution decreases over time.The relationship between biomass and potential of the solution could be expressed by the equation with constants of a and b whichare all related to species,batch,number,and growth environment of bacterial.When the initial pH value is10and the initial biomassis6.55×107cell/mL,the correlated equation of the biomass and the potential of the solution could be divided into two segments.Thegrowth of bacteria CH-1is different under various experimental conditions,but the biomass is directly related to the potential of thesolution regardless of the conditions of different initial pH values and bacteria number.展开更多
为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2...为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2或5%阿维菌素乳油375 m L/hm^2防治稻纵卷叶螟,且各种药剂对水稻安全。展开更多
文摘AIM: CMU-1 is a new preservation solution with a low potassium concentration as well as low viscosity that is highly effective in reducing preservation injury. The purpose of this experiment is to compare the protective effect of CMU-1 solution with that of UW during cold preservation and normothermic reperfusion. METHODS: Wistar rats were divided into two groups according to different preservation solution: CMU-1 group and UW group. After 6, 12 and 24 h cold storage of rat liver in different preservation solutions, the isolated perfused rat liver model was applied to reperfuse the liver for 120 min normothermically (37 癈) with Krebs-Henseleit solution, meanwhile the pH value of the preservation solution was measured. The perfusate was sampled for the evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). At the end of the reperfusion, all of the bile product was collected, energy metabolic substrate and histological examination were performed. RESULTS: After preserving for 6 h, pH value of both groups did not change; after 12 h, both decreased but with no significant difference. After 24 h, pH value in UW solution group significantly decreased. The total adenine nucleotides level and AEC in liver tissue decreased with preservation time, but they were higher in CMU-1 group. And the amount of bile product after perfusion for 120 min in CMU-1 group was much more than that in UW group. However, there were no significant differences in ALT and LDH levels between two groups. Histology showed no difference. CONCLUSION: The preservation effect of CMU-1 solution is similar with that of UW solution. However, CMU-1 solution shows some advantages over UW solution in energy metabolism, preventing intracellular acidosis and bile product.
基金Projects(51304251,51504299)supported by the National Natural Science Foundation of ChinaProject(201509050)supported by Special Program on Environmental Protection for Public Welfare,China
文摘A simple and effective method has been developed to reflect the growth of bacteria CH-1via the potential of the solution.The results indicate that during the bacterial cultivation,the biomass increases and the potential of the solution decreases over time.The relationship between biomass and potential of the solution could be expressed by the equation with constants of a and b whichare all related to species,batch,number,and growth environment of bacterial.When the initial pH value is10and the initial biomassis6.55×107cell/mL,the correlated equation of the biomass and the potential of the solution could be divided into two segments.Thegrowth of bacteria CH-1is different under various experimental conditions,but the biomass is directly related to the potential of thesolution regardless of the conditions of different initial pH values and bacteria number.
文摘为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2或5%阿维菌素乳油375 m L/hm^2防治稻纵卷叶螟,且各种药剂对水稻安全。