The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water ...The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water （WtF） from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl （DPPH） free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence （CL） assay and hydroxyl free radical-initiated chemiluminescence （CL） assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.展开更多
文摘The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water （WtF） from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl （DPPH） free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence （CL） assay and hydroxyl free radical-initiated chemiluminescence （CL） assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.
