目的研究脑微出血(cerebral microbleeds,CMBs)患者1,25-二羟维生素D3[1,25-dihydroxyvitamin D3,1,25-(OH)2D3],可溶性低密度脂蛋白受体相关蛋白1(soluble low density lipoprotein receptor related protein 1,sLRP1)水平与头颅SWI微...目的研究脑微出血(cerebral microbleeds,CMBs)患者1,25-二羟维生素D3[1,25-dihydroxyvitamin D3,1,25-(OH)2D3],可溶性低密度脂蛋白受体相关蛋白1(soluble low density lipoprotein receptor related protein 1,sLRP1)水平与头颅SWI微出血病灶数量及部位等影像学特征的相关关系。方法连续纳入2017年1月~2019年5月就诊于陕西省人民医院的CMBs患者196例(男性152例,女性44例),正常对照组99例(男性67例,女性32例),采集人口学资料及病史。对两组人群进行血浆1,25-(OH)2D3,sLRP1水平的检查。比较两组间血浆1,25-(OH)2D3和sLRP1水平的差异,并统计CMBs组各检验指标与CMBs病灶数量及部位的相关关系。结果CMBs组患者血浆1,25-(OH)2D3,sLRP1水平均低于对照组(23.32±18.91 mmol/L vs 39.60±18.58 mmol/L;237.96±70.62 ng/ml vs 312.61±62.78 ng/ml),差异均具有统计学意义(t=7.07,-9.24,均P<0.01)。CMBs患者血浆sLRP1水平与脑皮质CMBs病灶数量呈负相关(r=0.239,P=0.001),而与脑深部CMBs病灶数量无明显相关(t=-0.096,P>0.05)。结论CMBs患者的血浆1,25-(OH)2D3,sLRP1水平均低于正常人群。高表达的血浆sLRP1可能对脑皮质CMBs的发生具有一定的保护作用。展开更多
AIM: To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver transplantation.METHODS: Rats were randomly divided as donors or recipients for orthotopic ...AIM: To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver transplantation.METHODS: Rats were randomly divided as donors or recipients for orthotopic liver allotransplantation model. Four groups were designed in the study, Group Ⅰ: syngenic control (Wistar to Wistar); Group Ⅱ: acute rejection (SD to Wistar);Group Ⅲ: acute rejection treated with cyclosporine A, and Group Ⅳ: acute rejection treated with 1,25-(OH)2D3. Liver function, rejection activity index and mRNA of IFN-γ, IL-10intragraft in recipients were measured on day t, 5, 7, 15,30 posttransplant for assessing graft function, severity of acute rejection and immune state of recipients.RESULTS: Survival time of recipients in Group Ⅳ was significantly prolonged (4/6 recipients survived for over 100days. vs Group Ⅱ, P＜0.001; vs Group Ⅲ, P＞0.05). After treatment with 1,25-(OH)2 D3, mean value of all the assay tested on each experimental time was compared, liver function in group Ⅳ was significantly improved (AST 127±41U/L-360±104 U/L, BIL 13±5 mmol/1-38±11 mmol/l; vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05. Rejection activity index was significantly decreased (0-3.3±1.6; vsGroup Ⅱ, P＜0.05;vsGroup Ⅲ, P＞0.05). Level of hepatic IFN-γ, mRNA in group Ⅳ was decreased, while level of hepatic IL-10 mRNA was increased (vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05).CONCLUSION: Our results indicated that 1,25-(OH)2D3induced the secretion of cytokine toward to Th2 type, which would alleviate acute rejection, protect liver function and prolong survival of recipient after orthotopic liver transplantation.展开更多
Low-dose cytarabine combined with differentiating or DNA hypomethylating agents,such as vitamin D compounds,is a potential regimen to treat acute myeloid leukemia(AML)patients who are unfit for high-intensity chemothe...Low-dose cytarabine combined with differentiating or DNA hypomethylating agents,such as vitamin D compounds,is a potential regimen to treat acute myeloid leukemia(AML)patients who are unfit for high-intensity chemotherapy.The present study aimed to determine which subset of AML would be most responsive to low-dose cytarabine with the differentiating agent 1,25-dihydroxyvitamin D3(1,25-D3).Here,firstly,c Bio Portal database was used and we found out that vitamin D receptor(VDR)was highly expressed in acute monocytic leukemia(M5)and high VDR expression was associated with a poor survival of AML patients.Then,we confirmed that 1,25-D3 at clinical available concentration could induce more significant differentiation in acute monocytic leukemia cell lines(U937,MOLM-13,THP-1)and blasts from M5 patients than in non-monocytic cell lines(KG1 a and K562)and blasts from M2 patient.Finally,it was shown that the combination of 1,25-D3 and low-dose cytarabine further increased the differentiating rate,growth inhibition and G0/G1 arrest,while mild changes were found in the apoptosis in acute monocytic leukemia cell lines.Our study demonstrates that the enhanced response of acute monocytic leukemia cells to low-dose cytarabine by 1,25-D3 might indicate a novel therapeutic direction for patients with acute monocytic leukemia,especially for elderly and frail ones.展开更多
Objective:To explore the effect of 1,25-dihydroxyvitamin D3 on the mast cell tryptase(MCT) in asthmatic guinea pigs.Methods:A total of 60 male or female healthy guinea pigs were randomly divided into control group(gro...Objective:To explore the effect of 1,25-dihydroxyvitamin D3 on the mast cell tryptase(MCT) in asthmatic guinea pigs.Methods:A total of 60 male or female healthy guinea pigs were randomly divided into control group(group A),asthmatic group(group B).and 1,25-dihydroxyvitamin D3 group(group C),with 20 cases in each group.To establish asthmatic guinea pig models,1ml peanut oil was tilled into stomach in the morning in group A and group B.and 1 ml peanut oil with 1,25-dihydroxyvitamin D3 was filled into stomach in group C.Airway resistance(Re) of asthmatic guinea pigs was detected,and the bronchoalveolar lavage fluid(BALF) cells were counted.Lung tissue with HE and MCT immunohistochemical staining were used to observe the pathological changes in lung tissue and the distribution of MCT.Results:After injection of different concentration of acetylcholine chloride,the Re in group B and group C were increased significantly compared with group A(P<0.05):compared with group B.the Re in group C were decreased significantly(t=-5.385.-5.761.-6.184.-13.574.P<0.05):the total number of BALF cells and eosinophils were increased significantly in group B and C(t=19.618.9.598.10.854.5.388.P<0.05);compared with group B.the total number of BALF cells and eosinophils in group C was decreased significantly(t=-5.555.-5.392.P<0.05):the number of tryptase positive cells in group B was increased significantly than that in group A(t=21.312,P<0.05),and in addition to the alveolar septum and submucosa,the cells were also distributed around blood vessels and outside the cells:the number of tryptase positive cells in group C was decreased significantly compared with group B.and the difference was statistically significant(t=5.043.P<0.05).Conclusions:After the asthmatic guinea pigs arc treated with 1,25-dihydroxyvitamin D3,their BALF.Re.infiltration degree of inflammatory cells in the trachea and lung tissue and airway inflammatory reaction are reduced significantly.1,25-dihydroxyvitamin D3 has a certain inhibiting effect on the activation of mast cells and the release of MCT granules.展开更多
目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)...目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)及淋巴细胞亚群的量,并探讨其相互关系。方法采用电化学发光法检测受试者外周血1,25(OH)2D3的表达,流式细胞术检测患者T细胞百分比及CD3、CD4、CD8阳性细胞含量,采用RT-PCR方法检测皮损处VDR、miRNA155、SOCS1。结果湿疹患儿1,25(OH)2D3及VDR(29.25±11.03 nmol/L,1.74±0.92)明显低于健康儿童(55.49±21.66 nmol/L,3.62±1.68);湿疹患儿T细胞亚群68.65±16.74%及CD4+的T淋巴细胞亚群38.83±10.82%明显高于对照组(64.47±17.23,33.25±11.95%),CD8+的T淋巴细胞亚群25.68±6.81%明显低于对照组29.01±7.93%;湿疹患者皮损处VDR、SOCS的表达(1.74±0.92,1.78±1.02)明显低于对照组(3.62±1.68,3.13±1.70),miRNA1551的表达5.41±3.23明显高于对照组2.06±0.84。结论湿疹患儿1,25(OH)2D3、VDR表达异常,且其可调节湿疹患儿miR155/SOCS1通路。展开更多
文摘目的研究脑微出血(cerebral microbleeds,CMBs)患者1,25-二羟维生素D3[1,25-dihydroxyvitamin D3,1,25-(OH)2D3],可溶性低密度脂蛋白受体相关蛋白1(soluble low density lipoprotein receptor related protein 1,sLRP1)水平与头颅SWI微出血病灶数量及部位等影像学特征的相关关系。方法连续纳入2017年1月~2019年5月就诊于陕西省人民医院的CMBs患者196例(男性152例,女性44例),正常对照组99例(男性67例,女性32例),采集人口学资料及病史。对两组人群进行血浆1,25-(OH)2D3,sLRP1水平的检查。比较两组间血浆1,25-(OH)2D3和sLRP1水平的差异,并统计CMBs组各检验指标与CMBs病灶数量及部位的相关关系。结果CMBs组患者血浆1,25-(OH)2D3,sLRP1水平均低于对照组(23.32±18.91 mmol/L vs 39.60±18.58 mmol/L;237.96±70.62 ng/ml vs 312.61±62.78 ng/ml),差异均具有统计学意义(t=7.07,-9.24,均P<0.01)。CMBs患者血浆sLRP1水平与脑皮质CMBs病灶数量呈负相关(r=0.239,P=0.001),而与脑深部CMBs病灶数量无明显相关(t=-0.096,P>0.05)。结论CMBs患者的血浆1,25-(OH)2D3,sLRP1水平均低于正常人群。高表达的血浆sLRP1可能对脑皮质CMBs的发生具有一定的保护作用。
文摘AIM: To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver transplantation.METHODS: Rats were randomly divided as donors or recipients for orthotopic liver allotransplantation model. Four groups were designed in the study, Group Ⅰ: syngenic control (Wistar to Wistar); Group Ⅱ: acute rejection (SD to Wistar);Group Ⅲ: acute rejection treated with cyclosporine A, and Group Ⅳ: acute rejection treated with 1,25-(OH)2D3. Liver function, rejection activity index and mRNA of IFN-γ, IL-10intragraft in recipients were measured on day t, 5, 7, 15,30 posttransplant for assessing graft function, severity of acute rejection and immune state of recipients.RESULTS: Survival time of recipients in Group Ⅳ was significantly prolonged (4/6 recipients survived for over 100days. vs Group Ⅱ, P＜0.001; vs Group Ⅲ, P＞0.05). After treatment with 1,25-(OH)2 D3, mean value of all the assay tested on each experimental time was compared, liver function in group Ⅳ was significantly improved (AST 127±41U/L-360±104 U/L, BIL 13±5 mmol/1-38±11 mmol/l; vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05. Rejection activity index was significantly decreased (0-3.3±1.6; vsGroup Ⅱ, P＜0.05;vsGroup Ⅲ, P＞0.05). Level of hepatic IFN-γ, mRNA in group Ⅳ was decreased, while level of hepatic IL-10 mRNA was increased (vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05).CONCLUSION: Our results indicated that 1,25-(OH)2D3induced the secretion of cytokine toward to Th2 type, which would alleviate acute rejection, protect liver function and prolong survival of recipient after orthotopic liver transplantation.
基金supported by grants from the National Natural Science Foundation of China(No.81400172 and No.81470330)
文摘Low-dose cytarabine combined with differentiating or DNA hypomethylating agents,such as vitamin D compounds,is a potential regimen to treat acute myeloid leukemia(AML)patients who are unfit for high-intensity chemotherapy.The present study aimed to determine which subset of AML would be most responsive to low-dose cytarabine with the differentiating agent 1,25-dihydroxyvitamin D3(1,25-D3).Here,firstly,c Bio Portal database was used and we found out that vitamin D receptor(VDR)was highly expressed in acute monocytic leukemia(M5)and high VDR expression was associated with a poor survival of AML patients.Then,we confirmed that 1,25-D3 at clinical available concentration could induce more significant differentiation in acute monocytic leukemia cell lines(U937,MOLM-13,THP-1)and blasts from M5 patients than in non-monocytic cell lines(KG1 a and K562)and blasts from M2 patient.Finally,it was shown that the combination of 1,25-D3 and low-dose cytarabine further increased the differentiating rate,growth inhibition and G0/G1 arrest,while mild changes were found in the apoptosis in acute monocytic leukemia cell lines.Our study demonstrates that the enhanced response of acute monocytic leukemia cells to low-dose cytarabine by 1,25-D3 might indicate a novel therapeutic direction for patients with acute monocytic leukemia,especially for elderly and frail ones.
基金supported by Natural Science Foundation of Guangdong Province:gd291823
文摘Objective:To explore the effect of 1,25-dihydroxyvitamin D3 on the mast cell tryptase(MCT) in asthmatic guinea pigs.Methods:A total of 60 male or female healthy guinea pigs were randomly divided into control group(group A),asthmatic group(group B).and 1,25-dihydroxyvitamin D3 group(group C),with 20 cases in each group.To establish asthmatic guinea pig models,1ml peanut oil was tilled into stomach in the morning in group A and group B.and 1 ml peanut oil with 1,25-dihydroxyvitamin D3 was filled into stomach in group C.Airway resistance(Re) of asthmatic guinea pigs was detected,and the bronchoalveolar lavage fluid(BALF) cells were counted.Lung tissue with HE and MCT immunohistochemical staining were used to observe the pathological changes in lung tissue and the distribution of MCT.Results:After injection of different concentration of acetylcholine chloride,the Re in group B and group C were increased significantly compared with group A(P<0.05):compared with group B.the Re in group C were decreased significantly(t=-5.385.-5.761.-6.184.-13.574.P<0.05):the total number of BALF cells and eosinophils were increased significantly in group B and C(t=19.618.9.598.10.854.5.388.P<0.05);compared with group B.the total number of BALF cells and eosinophils in group C was decreased significantly(t=-5.555.-5.392.P<0.05):the number of tryptase positive cells in group B was increased significantly than that in group A(t=21.312,P<0.05),and in addition to the alveolar septum and submucosa,the cells were also distributed around blood vessels and outside the cells:the number of tryptase positive cells in group C was decreased significantly compared with group B.and the difference was statistically significant(t=5.043.P<0.05).Conclusions:After the asthmatic guinea pigs arc treated with 1,25-dihydroxyvitamin D3,their BALF.Re.infiltration degree of inflammatory cells in the trachea and lung tissue and airway inflammatory reaction are reduced significantly.1,25-dihydroxyvitamin D3 has a certain inhibiting effect on the activation of mast cells and the release of MCT granules.
文摘目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)及淋巴细胞亚群的量,并探讨其相互关系。方法采用电化学发光法检测受试者外周血1,25(OH)2D3的表达,流式细胞术检测患者T细胞百分比及CD3、CD4、CD8阳性细胞含量,采用RT-PCR方法检测皮损处VDR、miRNA155、SOCS1。结果湿疹患儿1,25(OH)2D3及VDR(29.25±11.03 nmol/L,1.74±0.92)明显低于健康儿童(55.49±21.66 nmol/L,3.62±1.68);湿疹患儿T细胞亚群68.65±16.74%及CD4+的T淋巴细胞亚群38.83±10.82%明显高于对照组(64.47±17.23,33.25±11.95%),CD8+的T淋巴细胞亚群25.68±6.81%明显低于对照组29.01±7.93%;湿疹患者皮损处VDR、SOCS的表达(1.74±0.92,1.78±1.02)明显低于对照组(3.62±1.68,3.13±1.70),miRNA1551的表达5.41±3.23明显高于对照组2.06±0.84。结论湿疹患儿1,25(OH)2D3、VDR表达异常,且其可调节湿疹患儿miR155/SOCS1通路。
