Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification techn...Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.展开更多
Objective： Invasive pulmonary candidiasis is a disease with high incidence, difficult treatment, poor prognosis, and high mortality. The present study analyzed the influence of cinnamaldehyde on 1,3-β-D-glucans in t...Objective： Invasive pulmonary candidiasis is a disease with high incidence, difficult treatment, poor prognosis, and high mortality. The present study analyzed the influence of cinnamaldehyde on 1,3-β-D-glucans in the cell wall of Candida albicans in order to provide a theoretical basis for the research of antifungal drugs.Methods： An immunosuppressed BALB/c mouse model with invasive pulmonary candidiasis was established by nasal perfusion of 50 μL of C. albicans suspension（107 cfu/mL）. 1,3-β-D-glucans examination and electron microscopy were carried out. Fluconazole was used as the control.Results： Cinnamaldehyde was administered at a dose of 240 mg/kg/d for 14 consecutive days, and the measured value of 1,3-β-D-glucans was（1160.62 ± 89.65） pg/mL, whereas that of fluconazole was（4285.87 ± 215.62） pg/m L. The difference between the two groups was statistically significant（P 〈 0.05）.Electron microscopy observation indicated that the 2-3 layers outside the cell wall of C. albicans（1,3-β-D-glucans layer） were rough, deformed, and incomplete, although the cell membrane was clear and intact.Conclusion： Cinnamaldehyde demonstrated special efficacy on 1,3-β-D-glucans in the cell wall of C. albicans.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22174102,21974101,22174101)the Sino-German rapid response funding call for Covid-19 related research(No.C-0008)。
文摘Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.
基金supported by Hebei Natural Science Foundation (H2013206316)
文摘Objective： Invasive pulmonary candidiasis is a disease with high incidence, difficult treatment, poor prognosis, and high mortality. The present study analyzed the influence of cinnamaldehyde on 1,3-β-D-glucans in the cell wall of Candida albicans in order to provide a theoretical basis for the research of antifungal drugs.Methods： An immunosuppressed BALB/c mouse model with invasive pulmonary candidiasis was established by nasal perfusion of 50 μL of C. albicans suspension（107 cfu/mL）. 1,3-β-D-glucans examination and electron microscopy were carried out. Fluconazole was used as the control.Results： Cinnamaldehyde was administered at a dose of 240 mg/kg/d for 14 consecutive days, and the measured value of 1,3-β-D-glucans was（1160.62 ± 89.65） pg/mL, whereas that of fluconazole was（4285.87 ± 215.62） pg/m L. The difference between the two groups was statistically significant（P 〈 0.05）.Electron microscopy observation indicated that the 2-3 layers outside the cell wall of C. albicans（1,3-β-D-glucans layer） were rough, deformed, and incomplete, although the cell membrane was clear and intact.Conclusion： Cinnamaldehyde demonstrated special efficacy on 1,3-β-D-glucans in the cell wall of C. albicans.
