High-energy diets and lipid metabolism can lead to high levels of total cholesterol,a decrease in antioxidant,enzyme activity,and an increase in oxidative stress and dyslipidemia biomarker.In this study,the in vivo an...High-energy diets and lipid metabolism can lead to high levels of total cholesterol,a decrease in antioxidant,enzyme activity,and an increase in oxidative stress and dyslipidemia biomarker.In this study,the in vivo antihyperlipidemic and antioxidant effects of 1,3-dioleoyl-2-palmitoyl triglyceride(OPO)using a high fat-diet model were investigated.The mice were divided into groups including high-fat model group(HF),auxiliary group(AG),positive Control group(PC),low OPO dose group(LO),medium OPO dose group(MO)and high OPO.OPO was administrated to 60 hyperlipidemia induced male Kunming mice at the dosage of 200,400 and 800 mg/kg•d body weight,for 35 days.The results showed that the administration of OPO decreased the body weight from 43.92 g in HF to 37.74 g in HO group,liver index from 3.94%in HF to 3.43 in HO group and kidney index from 1.38 in HF%to 1.17%in HO group.The administration of OPO significantly decreased hyperlipidemia mice's serum triglyceride,total cholesterol,and low-density lipoprotein cholesterol levels at P<0.05.High-density lipoprotein cholesterol levels were increased with reductions of visceral hypertrophy and fat accumulation in model mice.Furthermore,in the HO group,superoxide dismutase,catalase and glutathione peroxidase levels increased significantly by 27.39,38.33 and 22.90%at(P<0.05),respectively.OPO also significantly reduced the enzyme activity of aspartate aminotransferase and alanine aminotransferase in serum at P<0.05.These findings suggested that OPO has potential use in hyperlipidemia treatment and other health-related complications.展开更多
1,3-Dioleoyl-2-palmitoylglycerol(OPO)has been a hotspot of functional oils research in recent years,but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability,large...1,3-Dioleoyl-2-palmitoylglycerol(OPO)has been a hotspot of functional oils research in recent years,but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability,large-scale industrial application is difficult.In this study,the prepared magnetic ZnFe_(2)O_(4) was functionalized with dopamine to obtain ZnFe_(2)O_(4)@PDA,and the nano-biocatalyst ZnFe_(2)O_(4)@PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase(RML)via a cross-linking method.The existence of RML on ZnFe_(2)O_(4)@PDA was confirmed by XRD,FTIR,SEM,and TEM.This strategy proved to be simple and effective because the lipase immobilized on magnetic nanoparticles could be quickly recovered using external magnets,enabling reuse of the lipase.The activity,adaptability to a high temperature,pH value,and operational stability of immobilized RML were superior to those of free RML.After optimizing the synthesis conditions,the OPO yield was 42.78%,and the proportion of PA at the sn-2 position(PA-Sn2)was 54.63%.After the first four cycles,the activity of ZnFe_(2)O_(4)@PDA@RML was not significantly affected.The magnetically immobilized lipase has good thermal stability,long-term storage stability,reusability,and high catalytic activity.It can be used as a green and efficient biocatalyst to synthesize the OPO functional lipid.展开更多
基金the financial support received from the National Key Research and Development projects(2017YFD0400200).
文摘High-energy diets and lipid metabolism can lead to high levels of total cholesterol,a decrease in antioxidant,enzyme activity,and an increase in oxidative stress and dyslipidemia biomarker.In this study,the in vivo antihyperlipidemic and antioxidant effects of 1,3-dioleoyl-2-palmitoyl triglyceride(OPO)using a high fat-diet model were investigated.The mice were divided into groups including high-fat model group(HF),auxiliary group(AG),positive Control group(PC),low OPO dose group(LO),medium OPO dose group(MO)and high OPO.OPO was administrated to 60 hyperlipidemia induced male Kunming mice at the dosage of 200,400 and 800 mg/kg•d body weight,for 35 days.The results showed that the administration of OPO decreased the body weight from 43.92 g in HF to 37.74 g in HO group,liver index from 3.94%in HF to 3.43 in HO group and kidney index from 1.38 in HF%to 1.17%in HO group.The administration of OPO significantly decreased hyperlipidemia mice's serum triglyceride,total cholesterol,and low-density lipoprotein cholesterol levels at P<0.05.High-density lipoprotein cholesterol levels were increased with reductions of visceral hypertrophy and fat accumulation in model mice.Furthermore,in the HO group,superoxide dismutase,catalase and glutathione peroxidase levels increased significantly by 27.39,38.33 and 22.90%at(P<0.05),respectively.OPO also significantly reduced the enzyme activity of aspartate aminotransferase and alanine aminotransferase in serum at P<0.05.These findings suggested that OPO has potential use in hyperlipidemia treatment and other health-related complications.
基金This research was funded by the Science and Technology Program in Guangzhou City of China(Grant No.201904010087)the National College Students Innovation and Entrepreneurship Training Program of China(Grant No.202111347022)+2 种基金the Science and Technology Innovation Fund for Graduate Students(Grant No.KJCX2021005)Innovative Team Projects of Universities in Guangdong Province of China(Grant No.2016KCXTD003)2021 Guangdong University Research Platform and Scientific Research Project(Grant No.2021ZDZX2056).
文摘1,3-Dioleoyl-2-palmitoylglycerol(OPO)has been a hotspot of functional oils research in recent years,but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability,large-scale industrial application is difficult.In this study,the prepared magnetic ZnFe_(2)O_(4) was functionalized with dopamine to obtain ZnFe_(2)O_(4)@PDA,and the nano-biocatalyst ZnFe_(2)O_(4)@PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase(RML)via a cross-linking method.The existence of RML on ZnFe_(2)O_(4)@PDA was confirmed by XRD,FTIR,SEM,and TEM.This strategy proved to be simple and effective because the lipase immobilized on magnetic nanoparticles could be quickly recovered using external magnets,enabling reuse of the lipase.The activity,adaptability to a high temperature,pH value,and operational stability of immobilized RML were superior to those of free RML.After optimizing the synthesis conditions,the OPO yield was 42.78%,and the proportion of PA at the sn-2 position(PA-Sn2)was 54.63%.After the first four cycles,the activity of ZnFe_(2)O_(4)@PDA@RML was not significantly affected.The magnetically immobilized lipase has good thermal stability,long-term storage stability,reusability,and high catalytic activity.It can be used as a green and efficient biocatalyst to synthesize the OPO functional lipid.
