目的探讨T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨损伤中的诊断价值。方法对26例关节软骨损伤患者行T_2 star mapping、T_1 images和3D DESS扫描,并将T_1 images、T_2 star mapping与3D DESS图像融合,评价患者股骨...目的探讨T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨损伤中的诊断价值。方法对26例关节软骨损伤患者行T_2 star mapping、T_1 images和3D DESS扫描,并将T_1 images、T_2 star mapping与3D DESS图像融合,评价患者股骨、胫骨、髌骨关节软骨损伤程度并与关节镜结果对比,计算融合伪彩图诊断软骨损伤的特异性、敏感性及与关节镜诊断结果一致性。结果 T_1 images-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为92.8%、93.0%、0.769,T_2 star mapping-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为91.4%、94.2%、0.787。结论 T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨早期损伤评价上优于关节镜。展开更多
随着现代医疗手段的进步,肿瘤患者的生存期逐渐延长,癌症患者的远期生存质量越来越得到重视。蒽环类抗肿瘤药物应用于临床已达半世纪之久,在血液系统肿瘤及乳腺癌的治疗上目前仍然有着不可取代的地位。然而其所导致的心脏毒性亦对肿瘤...随着现代医疗手段的进步,肿瘤患者的生存期逐渐延长,癌症患者的远期生存质量越来越得到重视。蒽环类抗肿瘤药物应用于临床已达半世纪之久,在血液系统肿瘤及乳腺癌的治疗上目前仍然有着不可取代的地位。然而其所导致的心脏毒性亦对肿瘤患者的远期生存构成严重威胁。心脏磁共振(cardiac magnetic resonance,CMR)因其安全性高、具有可重复性等优点,已经成为评价心功能的金标准。除了CMR的常规指标外,一些尚未纳入欧洲心脏病学会(European society of cardiology,ESC)指南的新兴指标,如T1 mapping、T2 mapping等,更是可以从数值上直观、定量的评价出心肌细胞水肿及纤维化的程度,以便临床更精准的用药指导,这些新兴技术手段已经成为研究一大热点。本文现对T1、T2 mapping评价蒽环类药物所致心脏毒性的研究进展做一综述。展开更多
Rapid and accurate access to large-scale,high-resolution crop-type distribution maps is important for agricultural management and sustainable agricultural development.Due to the limitations of remote sensing image qua...Rapid and accurate access to large-scale,high-resolution crop-type distribution maps is important for agricultural management and sustainable agricultural development.Due to the limitations of remote sensing image quality and data processing capabilities,large-scale crop classification is still challenging.This study aimed to map the distribution of crops in Heilongjiang Province using Google Earth Engine(GEE)and Sentinel-1 and Sentinel-2 images.We obtained Sentinel-1 and Sentinel-2 images from all the covered study areas in the critical period for crop growth in 2018(May to September),combined monthly composite images of reflectance bands,vegetation indices and polarization bands as input features,and then performed crop classification using a Random Forest(RF)classifier.The results show that the Sentinel-1 and Sentinel-2 monthly composite images combined with the RF classifier can accurately generate the crop distribution map of the study area,and the overall accuracy(OA)reached 89.75%.Through experiments,we also found that the classification performance using time-series images is significantly better than that using single-period images.Compared with the use of traditional bands only(i.e.,the visible and near-infrared bands),the addition of shortwave infrared bands can improve the accuracy of crop classification most significantly,followed by the addition of red-edge bands.Adding common vegetation indices and Sentinel-1 data to the crop classification improved the overall classification accuracy and the OA by 0.2 and 0.6%,respectively,compared to using only the Sentinel-2 reflectance bands.The analysis of timeliness revealed that when the July image is available,the increase in the accuracy of crop classification is the highest.When the Sentinel-1 and Sentinel-2 images for May,June,and July are available,an OA greater than 80%can be achieved.The results of this study are applicable to large-scale,high-resolution crop classification and provide key technologies for remote sensing-based crop classification in small-scale agricultural areas.展开更多
为研究MAP2K1(MEK1)基因在三角帆蚌(Hyriopsis cumingii)性别决定中的作用,采用cDNA末端快速克隆技术(Rapid-amplification of cDNA ends,RACE)克隆了MAP2K1基因序列,利用实时荧光定量分析比较MAP2K1基因在三角帆蚌6个组织(性腺、闭壳...为研究MAP2K1(MEK1)基因在三角帆蚌(Hyriopsis cumingii)性别决定中的作用,采用cDNA末端快速克隆技术(Rapid-amplification of cDNA ends,RACE)克隆了MAP2K1基因序列,利用实时荧光定量分析比较MAP2K1基因在三角帆蚌6个组织(性腺、闭壳肌、肝胰腺、鳃、外套膜、斧足)、早期发育阶段(1—8月龄)性腺和1—3龄雌雄性腺中的表达水平,利用原位杂交确定MAP2K1基因在2龄三角帆蚌性腺中的定位。结果显示,MAP2K1基因开放阅读框(ORF)长度为1194 bp,编码397个氨基酸。MAP2K1基因在卵巢中高表达;早期发育阶段在2月龄表达量最高;1—3龄的表达结果显示,MAP2K1基因在卵巢中的表达量均高于同期精巢中的表达量(P<0.05)。原位杂交结果显示,MAP2K1基因在雌性三角帆蚌的卵母细胞及卵子上有明显的杂交信号。RNAi结果显示,干扰MAP2K1基因的上游基因C-MOS后,下游基因MAP2K1在雌性中的表达下降了82.31%,在雄性中的表达下降了73.60%。推测MAP2K1基因可能参与三角帆蚌的卵巢发育过程,在三角帆蚌中属于偏雌性基因,其表达受C-MOS基因的影响。展开更多
AIM To investigate the underlying molecularmechanisms of miR-451 to inhibit proliferation ofesophageal carcinoma cell line EC9706.METHODS： Assays for cell growth, apoptosis andinvasion were used to evaluate the effec...AIM To investigate the underlying molecularmechanisms of miR-451 to inhibit proliferation ofesophageal carcinoma cell line EC9706.METHODS： Assays for cell growth, apoptosis andinvasion were used to evaluate the effects of miR-451expression on EC cells. Luciferase reporter and Westernblot assays were used to test whether cyclin-dependentkinase inhibitor 2D （CDKN2D） and MAP3K1 act as majortargets of miR-451.RESULTS： The results showed that CDKN2D andMAP3K1 are direct targets of miR-451. CDKN2D andMAP3K1 overexpression reversed the effect of miR-451.MiR-451 inhibited the proliferation of EC9706 bytargeting CDKN2D and MAP3K1.CONCLUSION： These findings suggest that miR-451might be a novel prognostic biomarker and a potentialtarget for the treatment of esophageal squamous cellcarcinoma in the future.展开更多
A general mapping deformation method is presented and applied to a (2+1)-dimensional Boussinesq system. Many new types of explicit and exact travelling wave solutions, which contain solitary wave solutions, periodic w...A general mapping deformation method is presented and applied to a (2+1)-dimensional Boussinesq system. Many new types of explicit and exact travelling wave solutions, which contain solitary wave solutions, periodic wave solutions, Jacobian and Weierstrass doubly periodic wave solutions, and other exact excitations like polynomial solutions, exponential solutions, and rational solutions, etc., are obtained by a simple algebraic transformation relation between the (2+1)-dimensional Boussinesq equation and a generalized cubic nonlinear Klein-Gordon equation.展开更多
Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ...Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.展开更多
文摘目的探讨T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨损伤中的诊断价值。方法对26例关节软骨损伤患者行T_2 star mapping、T_1 images和3D DESS扫描,并将T_1 images、T_2 star mapping与3D DESS图像融合,评价患者股骨、胫骨、髌骨关节软骨损伤程度并与关节镜结果对比,计算融合伪彩图诊断软骨损伤的特异性、敏感性及与关节镜诊断结果一致性。结果 T_1 images-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为92.8%、93.0%、0.769,T_2 star mapping-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为91.4%、94.2%、0.787。结论 T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨早期损伤评价上优于关节镜。
文摘随着现代医疗手段的进步,肿瘤患者的生存期逐渐延长,癌症患者的远期生存质量越来越得到重视。蒽环类抗肿瘤药物应用于临床已达半世纪之久,在血液系统肿瘤及乳腺癌的治疗上目前仍然有着不可取代的地位。然而其所导致的心脏毒性亦对肿瘤患者的远期生存构成严重威胁。心脏磁共振(cardiac magnetic resonance,CMR)因其安全性高、具有可重复性等优点,已经成为评价心功能的金标准。除了CMR的常规指标外,一些尚未纳入欧洲心脏病学会(European society of cardiology,ESC)指南的新兴指标,如T1 mapping、T2 mapping等,更是可以从数值上直观、定量的评价出心肌细胞水肿及纤维化的程度,以便临床更精准的用药指导,这些新兴技术手段已经成为研究一大热点。本文现对T1、T2 mapping评价蒽环类药物所致心脏毒性的研究进展做一综述。
基金funded by the National Key R&D Program of China(2017YFD0201803)the Talent Recruitment Project of Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences.
文摘Rapid and accurate access to large-scale,high-resolution crop-type distribution maps is important for agricultural management and sustainable agricultural development.Due to the limitations of remote sensing image quality and data processing capabilities,large-scale crop classification is still challenging.This study aimed to map the distribution of crops in Heilongjiang Province using Google Earth Engine(GEE)and Sentinel-1 and Sentinel-2 images.We obtained Sentinel-1 and Sentinel-2 images from all the covered study areas in the critical period for crop growth in 2018(May to September),combined monthly composite images of reflectance bands,vegetation indices and polarization bands as input features,and then performed crop classification using a Random Forest(RF)classifier.The results show that the Sentinel-1 and Sentinel-2 monthly composite images combined with the RF classifier can accurately generate the crop distribution map of the study area,and the overall accuracy(OA)reached 89.75%.Through experiments,we also found that the classification performance using time-series images is significantly better than that using single-period images.Compared with the use of traditional bands only(i.e.,the visible and near-infrared bands),the addition of shortwave infrared bands can improve the accuracy of crop classification most significantly,followed by the addition of red-edge bands.Adding common vegetation indices and Sentinel-1 data to the crop classification improved the overall classification accuracy and the OA by 0.2 and 0.6%,respectively,compared to using only the Sentinel-2 reflectance bands.The analysis of timeliness revealed that when the July image is available,the increase in the accuracy of crop classification is the highest.When the Sentinel-1 and Sentinel-2 images for May,June,and July are available,an OA greater than 80%can be achieved.The results of this study are applicable to large-scale,high-resolution crop classification and provide key technologies for remote sensing-based crop classification in small-scale agricultural areas.
基金Supported by National Natural Science Foundation of China,No.81301726
文摘AIM To investigate the underlying molecularmechanisms of miR-451 to inhibit proliferation ofesophageal carcinoma cell line EC9706.METHODS： Assays for cell growth, apoptosis andinvasion were used to evaluate the effects of miR-451expression on EC cells. Luciferase reporter and Westernblot assays were used to test whether cyclin-dependentkinase inhibitor 2D （CDKN2D） and MAP3K1 act as majortargets of miR-451.RESULTS： The results showed that CDKN2D andMAP3K1 are direct targets of miR-451. CDKN2D andMAP3K1 overexpression reversed the effect of miR-451.MiR-451 inhibited the proliferation of EC9706 bytargeting CDKN2D and MAP3K1.CONCLUSION： These findings suggest that miR-451might be a novel prognostic biomarker and a potentialtarget for the treatment of esophageal squamous cellcarcinoma in the future.
文摘A general mapping deformation method is presented and applied to a (2+1)-dimensional Boussinesq system. Many new types of explicit and exact travelling wave solutions, which contain solitary wave solutions, periodic wave solutions, Jacobian and Weierstrass doubly periodic wave solutions, and other exact excitations like polynomial solutions, exponential solutions, and rational solutions, etc., are obtained by a simple algebraic transformation relation between the (2+1)-dimensional Boussinesq equation and a generalized cubic nonlinear Klein-Gordon equation.
文摘Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.
