目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡...目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡的作用及机制。方法 LIGHT、IFN-γ单独或联合处理MIN6细胞。分别运用CCK-8法、流式细胞术检测MIN6细胞增殖与凋亡率。Western blot检测信号转导和转录激活子1(signal transducers and activators of transcription 1,STAT1)及STAT1的磷酸化水平,以及凋亡与增殖相关基因Bcl-xL、Bax和增殖细胞核抗原(PCNA)的表达。MIN6细胞经STAT1特异的抑制剂弗达拉滨(fludarabine,Flu)预处理后再用LIGHT和IFN-γ联合处理,Western blot检测STAT1、p-STAT1、Bcl-xL、Bax的变化,CCK-8法检测MIN6细胞的增殖能力。结果 LIGHT协同IFN-γ呈时间依赖性抑制MIN6细胞增殖,诱导细胞凋亡,降低抗凋亡基因Bcl-xL表达、增加促凋亡基因Bax表达,对PCNA表达无明显影响;LIGHT促进IFN-γ激活STAT1,而STAT1抑制剂fludarabine抑制STAT1活化后,Bcl-xL表达上调,Bax表达下调,MIN6细胞增殖率上升。结论 LIGHT协同IFN-γ通过激活STAT1调节Bcl-xL、Bax表达而诱导小鼠胰岛β细胞株MIN6细胞凋亡。展开更多
By defining fuzzy valued simple functions and giving L1(μ) approximations of fuzzy valued integrably bounded functions by such simple functions, the paper analyses by L1(μ)-norm the approximation capability of four-...By defining fuzzy valued simple functions and giving L1(μ) approximations of fuzzy valued integrably bounded functions by such simple functions, the paper analyses by L1(μ)-norm the approximation capability of four-layer feedforward regular fuzzy neural networks to the fuzzy valued integrably bounded function F : Rn → FcO(R). That is, if the transfer functionσ: R→R is non-polynomial and integrable function on each finite interval, F may be innorm approximated by fuzzy valued functions defined as to anydegree of accuracy. Finally some real examples demonstrate the conclusions.展开更多
文摘目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡的作用及机制。方法 LIGHT、IFN-γ单独或联合处理MIN6细胞。分别运用CCK-8法、流式细胞术检测MIN6细胞增殖与凋亡率。Western blot检测信号转导和转录激活子1(signal transducers and activators of transcription 1,STAT1)及STAT1的磷酸化水平,以及凋亡与增殖相关基因Bcl-xL、Bax和增殖细胞核抗原(PCNA)的表达。MIN6细胞经STAT1特异的抑制剂弗达拉滨(fludarabine,Flu)预处理后再用LIGHT和IFN-γ联合处理,Western blot检测STAT1、p-STAT1、Bcl-xL、Bax的变化,CCK-8法检测MIN6细胞的增殖能力。结果 LIGHT协同IFN-γ呈时间依赖性抑制MIN6细胞增殖,诱导细胞凋亡,降低抗凋亡基因Bcl-xL表达、增加促凋亡基因Bax表达,对PCNA表达无明显影响;LIGHT促进IFN-γ激活STAT1,而STAT1抑制剂fludarabine抑制STAT1活化后,Bcl-xL表达上调,Bax表达下调,MIN6细胞增殖率上升。结论 LIGHT协同IFN-γ通过激活STAT1调节Bcl-xL、Bax表达而诱导小鼠胰岛β细胞株MIN6细胞凋亡。
基金Supported by the National Natural Science Foundation of China(No:69872039)
文摘By defining fuzzy valued simple functions and giving L1(μ) approximations of fuzzy valued integrably bounded functions by such simple functions, the paper analyses by L1(μ)-norm the approximation capability of four-layer feedforward regular fuzzy neural networks to the fuzzy valued integrably bounded function F : Rn → FcO(R). That is, if the transfer functionσ: R→R is non-polynomial and integrable function on each finite interval, F may be innorm approximated by fuzzy valued functions defined as to anydegree of accuracy. Finally some real examples demonstrate the conclusions.
