目的 探讨妊娠期肝病患者外周血25-羟基维生素D_(3)[25-hydroxy vitamin D_(3),25(OH)D_(3)]、高迁移率蛋白1(high mobility group box 1,HMGB1)、微小RNA-122(microRNA-122,miR-122)表达与凝血功能的相关性。方法 选取2019年2月至2021...目的 探讨妊娠期肝病患者外周血25-羟基维生素D_(3)[25-hydroxy vitamin D_(3),25(OH)D_(3)]、高迁移率蛋白1(high mobility group box 1,HMGB1)、微小RNA-122(microRNA-122,miR-122)表达与凝血功能的相关性。方法 选取2019年2月至2021年2月东营区人民医院收治的78例妊娠期肝病患者作为研究组,另选取同期78例健康孕妇作为对照组。比较两组25(OH)D_(3)、HMGB1、miR-122、凝血酶原时间(prothombin time,PT)、活化部分凝血活酶时间(activited partial thomboplastin time,APTT)、纤维蛋白原(fibrinogen,FIB)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、γ-谷氨酰转肽酶(γ-glutamyltranspeptidase,GGT)水平,分析25(OH)D_(3)、HMGB1、miR-122与凝血功能指标的相关性,采用受试者工作特征(receiver operating characteristic,ROC)曲线评价25(OH)D_(3)、HMGB1、miR-122与凝血功能指标对妊娠期肝病的评估价值,比较两组患者的妊娠结局。结果 研究组患者25(OH)D_(3)[(54.17±9.47)nmol/Lvs(65.98±11.02)nmol/L]和FIB[(2.74±0.69)g/L vs (5.31±1.49)g/L]水平低于对照组,HMGB1[(8.52±1.93)μg/L vs (6.17±1.59)μg/L]、miR-122 (13.49±4.07vs 8.28±2.50)、PT[(15.84±5.13)s vs (12.69±3.46)s]、APTT[(40.12±7.41)s vs (32.19±8.03)s]、ALT[(22.49±3.56)U/L vs (8.31±2.64)U/L]、AST[(78.29±12.75)U/L vs (21.69±7.28)U/L]、GGT[(42.49±11.16)U/L vs (20.69±6.24)U/L]水平高于对照组,差异有统计学意义(P均<0.05)。妊娠期肝病患者25(OH)D_(3)、FIB与ALT、AST、GGT呈负相关,HMGB1、miR-122、PT、APTT与ALT、AST、GGT呈正相关(P均<0.05)。妊娠期肝病患者25(OH)D_(3)与PT、APTT呈负相关,与FIB呈正相关,HMGB1、miR-122与PT、APTT呈正相关,与FIB呈负相关(P均<0.05)。25(OH)D_(3)、HMGB1、miR-122与凝血功能指标联合评估妊娠期肝病的ROC曲线下面积为0.884;研究组患者不良妊娠结局发生率高于对照组[16.67%(13/78) vs 2.56%(2/78);χ^(2)=8.925,P=0.003]。结论 妊娠期肝病患者外周血25(OH)D_(3)、HMGB1、miR-122水平与凝血功能间存在良好线性关系,对患者外周血25(OH)D_(3)、HMGB1、miR-122水平及凝血功能进行检测在妊娠期肝病评估方面具有一定辅助作用。展开更多
AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incub...AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.展开更多
Background:Coronary collateral circulation(CCC)plays a vital role in the myocardial blood supply,especially for ischemic myocardium.Evidence suggests that the visfatin and 25-hydroxyvitamin D_(3)[25(OH)D_(3)]levels ar...Background:Coronary collateral circulation(CCC)plays a vital role in the myocardial blood supply,especially for ischemic myocardium.Evidence suggests that the visfatin and 25-hydroxyvitamin D_(3)[25(OH)D_(3)]levels are related to the degree and incidence of vascular stenosis associated with coronary artery disease;however,few studies have evaluated the effect of visfatin and 25(OH)D_(3) on CCC development in patients with chronic total occlusion(CTO).This study aimed to evaluate the relationship between the serum visfatin and 25(OH)D_(3) levels and CCC in patients with CTO.Methods:A total of 189 patients with CTO confirmed by coronary angiography were included.CCC was graded from 0 to 3 according to the Rentrop-Cohen classification.Patients with grade 0 or grade 1 collateral development were in-cluded in the poor CCC group(n=82),whereas patients with grade 2 or grade 3 collateral development were included in the good CCC group(n=107).The serum visfatin and 25(OH)D_(3) levels were measured by ELISA.Results:The visfatin level was significantly higher in the poor CCC group than in the good CCC group,and the 25(OH)D_(3) level was significantly lower in the poor CCC group than in the good CCC group(P=0.000).Correlation analysis showed that the Rentrop grade was negatively correlated with the visfatin level(r=−0.692,P=0.000)but positively correlated with the 25(OH)D_(3) level(r=0.635,P=0.000).Logistic regression analysis showed that the vis-fatin and 25(OH)D_(3) levels were independent risk factors for CCC(odds ratio 1.597,95%confidence interval 1.300-1.961,P=0.000 and odds ratio 0.566,95%confidence interval 0.444-0.722,P=0.000,respectively).The visfatin and 25(OH)D_(3) levels can effectively predict the CCC status.Conclusion:Serum visfatin and 25(OH)D_(3) levels are related to CCC development and are independent predictors of poor CCC.展开更多
文摘目的 探讨妊娠期肝病患者外周血25-羟基维生素D_(3)[25-hydroxy vitamin D_(3),25(OH)D_(3)]、高迁移率蛋白1(high mobility group box 1,HMGB1)、微小RNA-122(microRNA-122,miR-122)表达与凝血功能的相关性。方法 选取2019年2月至2021年2月东营区人民医院收治的78例妊娠期肝病患者作为研究组,另选取同期78例健康孕妇作为对照组。比较两组25(OH)D_(3)、HMGB1、miR-122、凝血酶原时间(prothombin time,PT)、活化部分凝血活酶时间(activited partial thomboplastin time,APTT)、纤维蛋白原(fibrinogen,FIB)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、γ-谷氨酰转肽酶(γ-glutamyltranspeptidase,GGT)水平,分析25(OH)D_(3)、HMGB1、miR-122与凝血功能指标的相关性,采用受试者工作特征(receiver operating characteristic,ROC)曲线评价25(OH)D_(3)、HMGB1、miR-122与凝血功能指标对妊娠期肝病的评估价值,比较两组患者的妊娠结局。结果 研究组患者25(OH)D_(3)[(54.17±9.47)nmol/Lvs(65.98±11.02)nmol/L]和FIB[(2.74±0.69)g/L vs (5.31±1.49)g/L]水平低于对照组,HMGB1[(8.52±1.93)μg/L vs (6.17±1.59)μg/L]、miR-122 (13.49±4.07vs 8.28±2.50)、PT[(15.84±5.13)s vs (12.69±3.46)s]、APTT[(40.12±7.41)s vs (32.19±8.03)s]、ALT[(22.49±3.56)U/L vs (8.31±2.64)U/L]、AST[(78.29±12.75)U/L vs (21.69±7.28)U/L]、GGT[(42.49±11.16)U/L vs (20.69±6.24)U/L]水平高于对照组,差异有统计学意义(P均<0.05)。妊娠期肝病患者25(OH)D_(3)、FIB与ALT、AST、GGT呈负相关,HMGB1、miR-122、PT、APTT与ALT、AST、GGT呈正相关(P均<0.05)。妊娠期肝病患者25(OH)D_(3)与PT、APTT呈负相关,与FIB呈正相关,HMGB1、miR-122与PT、APTT呈正相关,与FIB呈负相关(P均<0.05)。25(OH)D_(3)、HMGB1、miR-122与凝血功能指标联合评估妊娠期肝病的ROC曲线下面积为0.884;研究组患者不良妊娠结局发生率高于对照组[16.67%(13/78) vs 2.56%(2/78);χ^(2)=8.925,P=0.003]。结论 妊娠期肝病患者外周血25(OH)D_(3)、HMGB1、miR-122水平与凝血功能间存在良好线性关系,对患者外周血25(OH)D_(3)、HMGB1、miR-122水平及凝血功能进行检测在妊娠期肝病评估方面具有一定辅助作用。
基金Supported by the Else Kroner-Fresenius Foundation, Bad Homburg, Germany
文摘AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.
基金supported by the Science Foundation of AMHT(2018-LCYL-009)the Medical and Health Research Project of China Aerospace Science and Industry Corporation Ltd.(KYLX-56).
文摘Background:Coronary collateral circulation(CCC)plays a vital role in the myocardial blood supply,especially for ischemic myocardium.Evidence suggests that the visfatin and 25-hydroxyvitamin D_(3)[25(OH)D_(3)]levels are related to the degree and incidence of vascular stenosis associated with coronary artery disease;however,few studies have evaluated the effect of visfatin and 25(OH)D_(3) on CCC development in patients with chronic total occlusion(CTO).This study aimed to evaluate the relationship between the serum visfatin and 25(OH)D_(3) levels and CCC in patients with CTO.Methods:A total of 189 patients with CTO confirmed by coronary angiography were included.CCC was graded from 0 to 3 according to the Rentrop-Cohen classification.Patients with grade 0 or grade 1 collateral development were in-cluded in the poor CCC group(n=82),whereas patients with grade 2 or grade 3 collateral development were included in the good CCC group(n=107).The serum visfatin and 25(OH)D_(3) levels were measured by ELISA.Results:The visfatin level was significantly higher in the poor CCC group than in the good CCC group,and the 25(OH)D_(3) level was significantly lower in the poor CCC group than in the good CCC group(P=0.000).Correlation analysis showed that the Rentrop grade was negatively correlated with the visfatin level(r=−0.692,P=0.000)but positively correlated with the 25(OH)D_(3) level(r=0.635,P=0.000).Logistic regression analysis showed that the vis-fatin and 25(OH)D_(3) levels were independent risk factors for CCC(odds ratio 1.597,95%confidence interval 1.300-1.961,P=0.000 and odds ratio 0.566,95%confidence interval 0.444-0.722,P=0.000,respectively).The visfatin and 25(OH)D_(3) levels can effectively predict the CCC status.Conclusion:Serum visfatin and 25(OH)D_(3) levels are related to CCC development and are independent predictors of poor CCC.
