蜂王浆是决定蜜蜂幼虫发育中级型分化,即成为蜂王还是工蜂的关键环境因素,而蜂王浆主蛋白(main royal jelly proteins,MRJPs)是反映蜂王浆新鲜度的重要指标。日本镰仓昌树以蜜蜂和果蝇为模型的最新研究表明,MRJP1是蜂王浆中决定蜜蜂级...蜂王浆是决定蜜蜂幼虫发育中级型分化,即成为蜂王还是工蜂的关键环境因素,而蜂王浆主蛋白(main royal jelly proteins,MRJPs)是反映蜂王浆新鲜度的重要指标。日本镰仓昌树以蜜蜂和果蝇为模型的最新研究表明,MRJP1是蜂王浆中决定蜜蜂级型分化的关键因子,该蛋白可通过激活虫体脂肪体中的表皮生长因子信号通路,引发个体增大、发育时间缩短和卵巢发育等蜂王特征的出现。因此,今后很有必要进一步开展MRJP1对人体的营养功能和作用机理研究,为MRJP1应用于功能食品提供科学依据。展开更多
Major royal jelly protein 1（MRJP1）, designated apalbumin 1, has been regarded as a freshness marker of royal jelly（RJ）. A MRJP1-specific peptide（IKEALPHVPIFD） identified by bioinformatics analysis of homologous ...Major royal jelly protein 1（MRJP1）, designated apalbumin 1, has been regarded as a freshness marker of royal jelly（RJ）. A MRJP1-specific peptide（IKEALPHVPIFD） identified by bioinformatics analysis of homologous members of the major royal protein family was synthesized and used to raise polyclonal anti-MRJP1 antibody（antiSP-MRJP1 antibody）. Western blot analysis showed that anti-SP-MRJP1 antibody only reacted with MRJP1 in RJ. In contrast, the previously reported antibody against recombinant MRJP1（anti-R-MRJP1 antibody） reacted with other members of MRJP family in RJ. Enzyme-linked immunosorbent assay（ELISA） using anti-SP-MRJP1 antibody demonstrated that MRJP1 content in RJ stored at 40 °C significantly degraded by 37.3%, 55.9%, 58.0%, 60.6%, 65.7%, 72.7%, and 73.1% at 7, 14, 21, 28, 35, 42, and 49 d, respectively, when compared with MRJP1 content in fresh RJ（0 d）. Optical density analysis of MRJP bands from sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） profiles demonstrated that the degradation of MRJP1, MRJP2, MRJP3, and MRJP5 in RJ was strongly and positively correlated with the period of storage（P〈0.0001）. Our results indicated anti-SP-MRJP1 antibody was highly specific for MRJP1, and ELISA using the antibody is a sensitive and easy-to-use method to determine the freshness and authenticity of RJ.展开更多
文摘蜂王浆是决定蜜蜂幼虫发育中级型分化,即成为蜂王还是工蜂的关键环境因素,而蜂王浆主蛋白(main royal jelly proteins,MRJPs)是反映蜂王浆新鲜度的重要指标。日本镰仓昌树以蜜蜂和果蝇为模型的最新研究表明,MRJP1是蜂王浆中决定蜜蜂级型分化的关键因子,该蛋白可通过激活虫体脂肪体中的表皮生长因子信号通路,引发个体增大、发育时间缩短和卵巢发育等蜂王特征的出现。因此,今后很有必要进一步开展MRJP1对人体的营养功能和作用机理研究,为MRJP1应用于功能食品提供科学依据。
基金supported by the Public Beneficial Scientific&Technical Plan of Zhejiang(No.2011C22039)the Important Scientific & Technical Plan of Zhejiang(No.2011C12023)+2 种基金the Important Scientific & Technical Innovation Project of Hangzhou(No.20131812A25)the Foundation of Fuli Institute of Food Science of Zhejiang University(No.KY201404)the National Natural Science Foundation of China(No.31271848)
文摘Major royal jelly protein 1（MRJP1）, designated apalbumin 1, has been regarded as a freshness marker of royal jelly（RJ）. A MRJP1-specific peptide（IKEALPHVPIFD） identified by bioinformatics analysis of homologous members of the major royal protein family was synthesized and used to raise polyclonal anti-MRJP1 antibody（antiSP-MRJP1 antibody）. Western blot analysis showed that anti-SP-MRJP1 antibody only reacted with MRJP1 in RJ. In contrast, the previously reported antibody against recombinant MRJP1（anti-R-MRJP1 antibody） reacted with other members of MRJP family in RJ. Enzyme-linked immunosorbent assay（ELISA） using anti-SP-MRJP1 antibody demonstrated that MRJP1 content in RJ stored at 40 °C significantly degraded by 37.3%, 55.9%, 58.0%, 60.6%, 65.7%, 72.7%, and 73.1% at 7, 14, 21, 28, 35, 42, and 49 d, respectively, when compared with MRJP1 content in fresh RJ（0 d）. Optical density analysis of MRJP bands from sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） profiles demonstrated that the degradation of MRJP1, MRJP2, MRJP3, and MRJP5 in RJ was strongly and positively correlated with the period of storage（P〈0.0001）. Our results indicated anti-SP-MRJP1 antibody was highly specific for MRJP1, and ELISA using the antibody is a sensitive and easy-to-use method to determine the freshness and authenticity of RJ.
