Alternative splicing and expression of the insulin-like growth factor (IGF-1) gene in osteoblasts under mechanical stretch

(IGF-1 ) 像胰岛素的生长因素 1 支持造骨细胞区别和骨头形成，和它的表示被机械段导致，因此， IGF-1 被认为连接机械刺激和本地织物回答的一个受动器分子。在这研究，一台机械拉长设备被设计把生理的水平静电干扰或周期的拉长刺激用于造骨细胞。IGF-1 mRNA 的不同 isoforms 被 RT-PCR 用各自的教材从房间放大，这些放大产品被定序。产品被发现被造骨细胞有选择地在拉长刺激下面生产的拼接的 IGF-1 的 iso 形式。ThisIGF-1 isoform 与原来在肌肉细胞被识别的 mechano 生长因素(MGF ) 有相同顺序。肝类型 IGF (L.IGF-1 ) 和在在段刺激下面的造骨细胞的 MGF 的表示的规定进一步用半量的 RT-PCR 被学习。Stretchstimulation 被发现 IGF-1 (L.IGF-1 和 MGF ) 的表示到职业人员警句，并且为两 isoformsexpression，更多被周期的段有效地比静态的段刺激。MGF 仅仅在受到机械段的造骨细胞被检测，建议 MGF 是段敏感生长因素。MGF 的表示比 L.IGF-1 的早达到顶点，它类似于他们在肌肉的规定并且作为在肌肉房间在骨头建议了 MGF 和 L.IGF-1 的类似的角色。在造骨细胞的 MGF 和 L.IGF-1 的功能将被进一步试验性的研究建立。

可控并流沉淀法制备草酸钴微细粉末研究

采用并流沉淀法制备出了粒度均匀、分散良好的棒状、树枝状和花簇状草酸钴微细晶粒。实验结果表明,草酸钴粒子具有一维延伸生长的特点,而反应物料浓度、温度、加料速度、底液水量、陈化时间和搅拌与否等反应条件均不能改变其生长特性,仅能影响产物粒子的大小和分散性。分析确定沉淀物的化学成分为CoC2O4.2H2O,在空气中于330℃左右热分解可得到Co3O4粉末,在930℃左右则可得到CoO粉末。

Expression and subcellular localization of mechano-growth factor in osteoblasts under mechanical stretch

Mechano-growth factor (MGF) is a stretch sensitive factor in myocytes, and it might also be produced by other mechanocytes under mechanical stimulation. In this study, both the mRNA and protein expression of MGF were detected in stretched osteoblasts. Quantitative analysis showed that a cyclic stretching stimulation caused a quick and sharp increase of MGF mRNA and protein expression from a low basal level under no stretch; the mRNA and protein levels respectively peaked in 6 and 12 h to 5 and 5.2 fold over the basal level and returned to normal by 24 h. The subcellular distribution of MGF protein was revealed by immunofluorescence analysis to be restricted to the nucleus. We concluded that cyclic stretching stimulation could induce MGF expression in osteoblasts in a pulsing fashion; and the nuclear distribution of MGF suggested that MGF might act in mechanocytes as an autocrine growth factor.