用二苯基苦基苯肼(DPPH)自由基酶标仪法,对拟青霉属5个种的20个菌株培养液提取物的自由基清除活性进行了比较研究,发现不同种拟青霉的甲醇提取液的自由基清除活性差异很大,在浓度为5.0 mg.mL-1,37℃保温10 m in时,自由基清除活性最高为7...用二苯基苦基苯肼(DPPH)自由基酶标仪法,对拟青霉属5个种的20个菌株培养液提取物的自由基清除活性进行了比较研究,发现不同种拟青霉的甲醇提取液的自由基清除活性差异很大,在浓度为5.0 mg.mL-1,37℃保温10 m in时,自由基清除活性最高为71.9%,最低为15.9%;同种不同菌株之间的活性也有较大的差异。自由基清除活性均与反应时间和样品浓度成正相关关系。二苯基苦基苯肼自由基薄层实验结果表明,该属菌株培养液提取物中自由基清除剂都不是单一成分,且组成也不完全相同。展开更多
研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比...研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2.)、羟自由基(.OH)和烷基自由基的清除能力,同时测定其体外总抗氧化能力。结果表明,AMNL对DPPH自由基的清除能力(IC50=9.07mg/L)显著高于AMAL与AMALV,弱于芦丁和VC。AMNL对O2.(IC50=2.82mg/L)和.OH(IC50=7.77mg/L)的清除能力不仅明显高于AMAL与AMALV,而且清除效果也优于芦丁和VC。各桑椹花青素对烷基自由基清除率均低于50%。AMNL总抗氧化能力分别是AMAL、AMALV及芦丁的11.8倍、2.6倍及1.3倍,但低于VC。展开更多
The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and t...The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and the presence of some compounds. The FT-IR reveals the AgNP<sub>s</sub> capping and reducing the particular biomolecule from the functional group for identification. Compounds found in the FT-IR seeds of Capsicum annum are Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol). Monodora myristica are Mo L (Molybdenum), Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol), Mg K (Magnesium). Piper guineense are Ag L (Silver iodide), Ci K (Potassium chloride), C K (Cyanogen chloride), P K (Phenol). The seeds show that the AgNP<sub>S</sub> of CA and MM has a better antioxidant activity than the methanol of CA and MM, while the PG methanol has a better activity than the AgNP<sub>S</sub> PG. The control (Catechin and Galic acid) has a slight overall better DPPH activity than the AgNP<sub>S</sub>. It is important to note that there is a concentration dependency in CA, MM AgNP<sub>S,</sub> PG methanol respectively. Notably, at CA methanol, the conc. at 125 was higher than the conc. at 250. Hence, there is need to create a great part in using plant samples for making tabulated or capsulated drugs for treatment of diseases and using plant silver nanoparticles to develop a healthy food/drug preservative package material “smart packaging” that will enhance shelf-life.展开更多
Turmeric is a spice widely used to enhance the taste and color of certain meats. We investigated the antioxidant capacities of turmeric ethanol soaking extracts by utilizing the scavenging properties of hydroxyl radic...Turmeric is a spice widely used to enhance the taste and color of certain meats. We investigated the antioxidant capacities of turmeric ethanol soaking extracts by utilizing the scavenging properties of hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl（DPPH） free radical. The results showed that the optimum extraction parameters were： extraction time 4 h, sample-solvent ratio 1：84.12, solvent 72.34% ethanol solution, and water bath temperature 81.3 ℃. The optimum ethanol soaking extraction parameters were： extraction time 4 h, sample-solvent ratio 1：90.74, solvent 80.46% ethanol solution, water bath temperature 88.2 ℃. With these parameters, the hydroxyl radical scavenging rate and the DPPH free radical clearance of crude extracts from turmeric can reach about 93.78% and 40.69%, respectively. These results indicate that ethanol soaking extraction may be a useful method for extracting antioxidants from turmeric.展开更多
Objective:To analyze the methanol extract of Trigonella foenum-graecum(T.foenum-graecum)for antioxidant,phytotoxic and cytotoxic activity.Methods:The powder of T.foenum-graecum was extracted in diluted methanol with t...Objective:To analyze the methanol extract of Trigonella foenum-graecum(T.foenum-graecum)for antioxidant,phytotoxic and cytotoxic activity.Methods:The powder of T.foenum-graecum was extracted in diluted methanol with the help of random shaking method.All extracts of the plant were measured for cytotoxic activity(beside brine shrimp and antioxidant activity vs.1,1-diphenyl-2-picrylhydrazyl free radical).Results:Various concentrations of methanolic extract of T.foenum-graecum were observed as 36.16%to 54.12%with rising concentrations of 50 to 1000μg/mL.Significantly phytotoxic activity(100 and 1000μg/mL)reduced the growth of roots(radicals)and shoots(hypocotyls)of rice when compared to control after 3 and 7 days’treatment.At a concentration of 10μg/mL,the survival rate of cytotoxic activity of brine shrimp was maximum and at a concentration of 250μg/mL,the death rate of brine shrimp was maximum.Conclusions:T.foenum-graecum has potential activity against free radical mediated sickness and thus it is possible to treat cancer.展开更多
文摘用二苯基苦基苯肼(DPPH)自由基酶标仪法,对拟青霉属5个种的20个菌株培养液提取物的自由基清除活性进行了比较研究,发现不同种拟青霉的甲醇提取液的自由基清除活性差异很大,在浓度为5.0 mg.mL-1,37℃保温10 m in时,自由基清除活性最高为71.9%,最低为15.9%;同种不同菌株之间的活性也有较大的差异。自由基清除活性均与反应时间和样品浓度成正相关关系。二苯基苦基苯肼自由基薄层实验结果表明,该属菌株培养液提取物中自由基清除剂都不是单一成分,且组成也不完全相同。
文摘研究药桑椹花青素(anthocyanins from Morus nigra L.,AMNL)的体外抗氧化作用,并与白桑椹花青素(anthocyanins from Morus alba L.,AMAL)和黑桑椹花青素(anthocyanins from Morus alba Linn.var.tatarica,AMALV)的体外抗氧化作用进行比较。以芦丁和VC为对照,测定AMNL对二苯代苦味酰肼自由基(DPPH自由基)、超氧阴离子自由基(O2.)、羟自由基(.OH)和烷基自由基的清除能力,同时测定其体外总抗氧化能力。结果表明,AMNL对DPPH自由基的清除能力(IC50=9.07mg/L)显著高于AMAL与AMALV,弱于芦丁和VC。AMNL对O2.(IC50=2.82mg/L)和.OH(IC50=7.77mg/L)的清除能力不仅明显高于AMAL与AMALV,而且清除效果也优于芦丁和VC。各桑椹花青素对烷基自由基清除率均低于50%。AMNL总抗氧化能力分别是AMAL、AMALV及芦丁的11.8倍、2.6倍及1.3倍,但低于VC。
文摘The antioxidant of seeds was carried out using extracts from methanol and Silver Nanoparticles from the spice. The SEM shows the shapes, dispersion and agglomeration of the sample, while the EDX confirms the SEM and the presence of some compounds. The FT-IR reveals the AgNP<sub>s</sub> capping and reducing the particular biomolecule from the functional group for identification. Compounds found in the FT-IR seeds of Capsicum annum are Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol). Monodora myristica are Mo L (Molybdenum), Ag L (Silver iodide), C K (Cyanogen chloride), P K (Phenol), Mg K (Magnesium). Piper guineense are Ag L (Silver iodide), Ci K (Potassium chloride), C K (Cyanogen chloride), P K (Phenol). The seeds show that the AgNP<sub>S</sub> of CA and MM has a better antioxidant activity than the methanol of CA and MM, while the PG methanol has a better activity than the AgNP<sub>S</sub> PG. The control (Catechin and Galic acid) has a slight overall better DPPH activity than the AgNP<sub>S</sub>. It is important to note that there is a concentration dependency in CA, MM AgNP<sub>S,</sub> PG methanol respectively. Notably, at CA methanol, the conc. at 125 was higher than the conc. at 250. Hence, there is need to create a great part in using plant samples for making tabulated or capsulated drugs for treatment of diseases and using plant silver nanoparticles to develop a healthy food/drug preservative package material “smart packaging” that will enhance shelf-life.
基金Supported by the National Natural Science Foundation of China(31401500)the Natural Science Foundation of Zhejiang Province(LQ13C200005)the Young Academic Team of Zhejiang Shuren University
文摘Turmeric is a spice widely used to enhance the taste and color of certain meats. We investigated the antioxidant capacities of turmeric ethanol soaking extracts by utilizing the scavenging properties of hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl（DPPH） free radical. The results showed that the optimum extraction parameters were： extraction time 4 h, sample-solvent ratio 1：84.12, solvent 72.34% ethanol solution, and water bath temperature 81.3 ℃. The optimum ethanol soaking extraction parameters were： extraction time 4 h, sample-solvent ratio 1：90.74, solvent 80.46% ethanol solution, water bath temperature 88.2 ℃. With these parameters, the hydroxyl radical scavenging rate and the DPPH free radical clearance of crude extracts from turmeric can reach about 93.78% and 40.69%, respectively. These results indicate that ethanol soaking extraction may be a useful method for extracting antioxidants from turmeric.
文摘Objective:To analyze the methanol extract of Trigonella foenum-graecum(T.foenum-graecum)for antioxidant,phytotoxic and cytotoxic activity.Methods:The powder of T.foenum-graecum was extracted in diluted methanol with the help of random shaking method.All extracts of the plant were measured for cytotoxic activity(beside brine shrimp and antioxidant activity vs.1,1-diphenyl-2-picrylhydrazyl free radical).Results:Various concentrations of methanolic extract of T.foenum-graecum were observed as 36.16%to 54.12%with rising concentrations of 50 to 1000μg/mL.Significantly phytotoxic activity(100 and 1000μg/mL)reduced the growth of roots(radicals)and shoots(hypocotyls)of rice when compared to control after 3 and 7 days’treatment.At a concentration of 10μg/mL,the survival rate of cytotoxic activity of brine shrimp was maximum and at a concentration of 250μg/mL,the death rate of brine shrimp was maximum.Conclusions:T.foenum-graecum has potential activity against free radical mediated sickness and thus it is possible to treat cancer.
