The Transient Time-Resolved Absorption Spectra of 1-Naphthylacetic Acid

The transient absorption spectra of 1-naphthylacetic acid in tetrahydrofuran have been obtained by laser flash photolysis technique. The transient species bands are at 509, 535 and 553 nm. These peak intensities increase in the maximum values at 10.4 mu s.

Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

BACKGROUND Leukemia stem cells(LSCs)are found to be one of the main factors contributing to poor therapeutic effects in acute myeloid leukemia(AML),as they are protected by the bone marrow microenvironment(BMM)against conventional therapies.Gossypol acetic acid(GAA),which is extracted from the seeds of cotton plants,exerts anti-tumor roles in several types of cancer and has been reported to induce apoptosis of LSCs by inhibiting Bcl2.AIM To investigate the exact roles of GAA in regulating LSCs under different microenvironments and the exact mechanism.METHODS In this study,LSCs were magnetically sorted from AML cell lines and the CD34+CD38-population was obtained.The expression of leucine-rich pentatricopeptide repeat-containing protein(LRPPRC)and forkhead box M1(FOXM1)was evaluated in LSCs,and the effects of GAA on malignancies and mitochondrial RESULTS LRPPRC was found to be upregulated,and GAA inhibited cell proliferation by degrading LRPPRC.GAA induced LRPPRC degradation and inhibited the activation of interleukin 6(IL-6)/janus kinase(JAK)1/signal transducer and activator of transcription(STAT)3 signaling,enhancing chemosensitivity in LSCs against conventional chemotherapies,including L-Asparaginase,Dexamethasone,and cytarabine.GAA was also found to downregulate FOXM1 indirectly by regulating LRPPRC.Furthermore,GAA induced reactive oxygen species accumulation,disturbed mitochondrial homeostasis,and caused mitochondrial dysfunction.By inhibiting IL-6/JAK1/STAT3 signaling via degrading LRPPRC,GAA resulted in the elimination of LSCs.Meanwhile,GAA induced oxidative stress and subsequent cell damage by causing mitochondrial damage.CONCLUSION Taken together,the results indicate that GAA might overcome the BMM protective effect and be considered as a novel and effective combination therapy for AML.

Isoimperatorin alleviates acetic acid-induced colitis in rats

Objective:To investigate the effect of isoimperatorin on histopathological and biochemical changes in acetic acid-induced colitis rats.Methods:Colitis was induced by intracolonic administration of acetic acid solution(4%v/v)in rats.Rats were divided into six groups including the sham group,the negative control group,the dexamethasone-treated group,and the groups treated with isoimperatorin(0.1,1,and 10 mg/kg/d by gavage).The treatments were administered for three days and then colonic status was assessed by macroscopic,histopathological,and biochemical analyses.Results:Isoimperatorin significantly alleviated colonic damage in a dose-dependent manner and improved histological changes in rats with acetic acid-induced colitis.It also significantly reduced myeloperoxidase,TNF-α,IL-1β,and malodialdehyde levels.Conclusions:Isoimperatorin alleviates acetic acid-induced colitis in rats and may be a potential therapeutic agent for the treatment of colitis.

Pharmacological Study on Antitumor Activity of 5-Fluorouracil-1-Acetic Acid and Its Rare Earth Complexes

The antitumor activity of 5 fluorouracil 1 acetic acid(HFAA) and its lanthanide complexes(La(FAA) 3, Eu(FAA) 3) were studied. The results show that HFAA, La(FAA) 3 and Eu(FAA) 3 with the concentrations of 1 0×10 -5 ～1 0×10 -2 μg·ml -1 inhibit the colony formation of leukemia cells(L 1210 ) and the growth of transplanted tumor sarcoma 180(S 180 ), hepatic carcinoma(HEPA) and ehrlich ascites tumor(EC) as well. The maximum inhibitory rate of Eu(FAA) 3 for S 180 is 38 4%, that HFAA and La(FAA) 3 for EC are 22 4% and 43 4%, respectively. The life prolongation rate of Eu(FAA) 3 for HEPA bearing mice is as long as 284%.

Preparation,Crystal Structure,Thermal Decomposition and Explosive Properties of K_2(tza)_2(H_2O) (tza=Tetrazole-1-acetic Acid)

A new energetic compound based on the tetrazole-1-acetic acid （tza） and potassium（I） salt, K2（tza）2（H2O）, was synthesized and characterized by elemental analysis and FT-IR spectrum. Its crystal structure was determined by single-crystal X-ray diffraction analysis. The results show that the crystal belongs to the orthorhombic system, space group Pna21 with a = 1.11972（17） nm, b = 0.46647（7） nm, c = 2.5158（4） nm, V = 1.3140（3） nm3, K2C6H8N8O5, Mr = 350.40 g·mol-1, Dc = 1.771 g·cm^-3, μ（MoKα） = 0.759 mm^-1, F（000） = 712, Z = 4, R = 0.023 and wR = 0.0527 for 2961 observed reflections （I 〉 2σ（I））. The K（I） cation is six-coordinated with four O atoms from three carboxylate groups, one O atom from one H2O molecule and one N atom from tetrazolyl ring, in which each tza is coordinated in a tridentate chelating bridging coordination mode. The thermal decomposition mechanism of the title complex was studied by DSC and TG-DTG techniques. Under nitrogen atmosphere at a heating rate of 10 K·min-1, the thermal decomposition of the complex contains one main exothermic process between 191.7 and 243.8 ℃ in the DSC curve. Its combustion heat was mensurated by oxygen bomb calorimetry. The non-isothermal kinetics parameters were calculated by the Kissinger＇s method and Ozawa-Doyle＇s method, respectively. The sensitivity properties of K2（tza）2（H2O） were also determined with standard methods, which was very sensitive to flame.

Synthesis,Structure,and Surface Photo-electric Properties of a New Zn(Ⅱ)Coordination Complex Constructed from 2-(1H-benzotriazol-1-yl)acetic Acid and 1,10-Phenanthroline Ligands

A new binuclear Zn^Ⅱ coordination complex,Zn2（bta）（phen）2（Cl）3（1,Hbta = 2-（1Hbenzotriazol-1-yl）acetic acid and phen = 1,10-phenanthroline）,has been synthesized and characterized by single-crystal X-ray diffraction,IR spectroscopy,elemental,and photoluminescent analysis.Complex 1 crystallizes in triclinic system,space group P1 with a = 9.3040（19）,b = 10.694（2）,c =16.841（3） A°,α = 101.18（3）,β = 105.77（3）,γ = 91.72（3）°,V= 1575.8（5） A°3,C（32）H（22）Zn2Cl3N7O2,Mr =773.66,Dc = 1.631 g/cm^3,Z = 2,F（000） = 780,μ = 1.820 mm^-1,the final R = 0.1238 and wR =0.1131.X-ray diffraction analyses indicate that 1 displays two crystallographic independent Zn^Ⅱmetal centers with a distorted tetragonal pyramidal（ZnN4O） and a tetrahedral（ZnNCl3） geometries,respectively.The phen serves as a common N,N＇-bidentate ligand,and the bta^- as a unique N,O-bridged ligand in 1.In the crystal,1 forms a stable 3D supramolecular architecture by trifurcated hydrogen bonding C-H…C1 interactions and C-H…π,π…π stacking.1 showed photo-electric conversion properties.

Improving Effect of Acetic Acid Bacteria (Gluconacetobacter hansenii GK-1) on sIgA and Physical Conditions in Healthy People: Double-Blinded Placebo-Controlled Study

It is known that the consumption of bacteria such as lactobacilli and bifidobacteria has beneficial effects on human immune function. Most of them are Gram-positive bacteria, and there are few reports on Gram-negative bacteria. In this study, we evaluated the effects of intake of Gluconacetobacter hansenii GK-1 (GK-1), Gram-negative acetic acid bacteria, for 12 weeks on physical condition and immune indices. We conducted a randomized, double-blind, placebo-controlled, parallel-group study in 100 healthy adults. The subjects were randomized into the GK-1 and the placebo groups. The diary-administered physical condition survey was conducted during the study period. The evaluation of salivary sIgA levels, NK-cell activity, and serum IFN-γ levels and quality of life survey was conducted before, in 6 weeks, and 12 weeks after the start of ingestion. Based on the physical condition survey, the cumulative onset-days of symptoms were significantly suppressed in the GK-1 group compared to the placebo group regarding the evaluation of 13 symptoms related to immunity, every 3 weeks. Additionally, salivary sIgA levels per hour were significantly increased in the GK-1 group compared with the placebo group at 6 and 12 weeks. Despite no significant differences in the NK-cell activity, serum IFN-γ levels or quality of life survey between the groups. Serum IFN-γ levels in the GK-1 group were significantly elevated at 12 weeks after the start of ingestion compared with those before ingestion. In conclusion, intake of GK-1 was shown to increase salivary sIgA levels and improve physical condition. This suggested that oral intake of GK-1 may help maintain the immune system.

Preparation of La-Mo-V mixed-oxide systems and their application in the direct synthesis of acetic acid

In this study, mixed metal oxides developed with a perovskite-type structure that show great potential for use in catalysis. Perovskite oxide catalysts with the composition LaMoxV1-xOn （x = 0.1, 0.3, 0.5, 0.7, and 0.9） have been synthesized by the sol-gel method and then used in the ethane dry reforming reaction for the direct synthesis of acetic acid. The influence of the nature of the metallic source （metal, nitrate, acetylacetonate, and ammonium） on gel formation has been studied by Fourier-transform infrared spectroscopy （FT-IR） and thermogravimetric analysis （TGA-DTA）. After calcination, the obtained perovskites were characterized by X-ray diffraction （XRD） and energy-dispersive X-ray spectrometry （EDS） coupled with scanning electron microscopy （SEM）. The catalysts were then subjected to thermo-programmed reduction （TPR）. The surface area （BET） was found to increase from 2.6 m^2/g （x = 0.1） to 5.1 m2/g （x = 1.0） with increasing molybdenum content following calcinations at 750 °C, and pure LaMoxV1-xOn perovskite was obtained with good homogeneity. The catalysts have been characterized by XRD, SEM, EDS, and carbon analysis （CA）. The results indicate that through this synthesis it is possible to obtain highly crystalline, homogeneous and pure solids, with well-defined structures. The direct synthesis of acetic acid from ethane over the perovskite catalysts was studied at temperatures between 450 and 850 °C and elevated pressures between 1 and 8 bar. It was found that the yield of acetic acid and the selectivity of its formation could be increased by incorporating more molybdenum into the perovskite structure. The experimental studies have shown that the calcination temperature and the molybdenum content have a significant influence on the catalytic activity. Amongst the catalysts tested, LaMo0.7V0.3O4.2 exhibited the best activity and stability.

Synergistic action of famotidine and chlorpheniramine on acetic acid-induced chronic gastric ulcer in rats

AIM： To assess the synergistic action of famotidine （FMD） and chlorpheniramine （CPA） on acetic acid-induced chronic gastric ulcer in rats. METHODS： Chronic gastric lesions were induced in male Sprague-Dawley （SD） rats by serosal application of the acetic acid. Forty SD rats were randomly divided into blank group （n = 8）, control group （n = 8）, FMD group （n = 8）, CPA group （n = 8）, and FMD＋CPA group （n = 8）. Each group was given intraperitoneally （i.p.） 0.5 mL/100 g distilled water, 9 g/L NaCl saline, 4 mg/kg FMD, 10 mg/kg CPA, 4 mg/kg FMD＋10 mg/kg CPA, respectively, daily for 10 d, On d 10, ulcer area was determined by planimetry, The level of myeloperoxidase （MPO） in the liver homogenation was determined by biochemical methods and the plasma levels of 6-ketoprostaglandin F1 alpha （6-keto-PGFla）and IL-8 were determined by radioimmunoassay. RESULTS： The synergistic effects of FMD＋CPA group on the lesion, IL-8, 6-keto-PGFla and MPO were confirmed. The effect of FMD＋CPA group was significantly different as compared to the control and FMD groups. The lesion （mm2） was reduced from 40.18±2.6 in control group to 6.83±2.97 in PMD＋CPA group, P〈0.01, and from 32.9 ±3.27 in FMD group to 6.83±2.97 in PMD＋CPA group, P〈0.01. The plasma levels of IL-8 decreased from 0.69± 0.11 ng/L in control group to 0.4±0.04 ng/L in PMD＋CPA group, P〈0.01, and from 0.51±0.08 ng/L in FMD group to 0.4±0.04 ng/L in PMD＋CPA group, P〈0.05. The level of 6-keto-PGF1, increased from 7.55±1.65 ng/L in control group to 16.62±0.97 ng/L in PMD＋CPA group,P〈0.01, and from 13.15±1.48 ng/L in FMD group to 16.62±0.97 ng/L in PMD＋CPA group,P〈0.05. The levels of MPO in the liver homogenate decreased from 9.12±2.05 u/Lin control group to 4.33±0.95 u/L in PMD＋CPA group, P〈0.01, and from 8.3±1.29 u/L in FMD group to 4.33±0.95 u/L, P〈0.01. CONCLUSION： The synergistic action of FMD and CPA on acetic acid-induced chronic gastric ulcer in rats decreases the incidence of ulcer and also enhances the healing of ulcer.

Multi-walled carbon nanotube as a solid phase extraction adsorbent for analysis of indole-3-butyric acid and 1-naphthylacetic acid in plant samples

In this work, a new sample pretreatment method prior to HPLC separations was developed for the determination of auxins in plant samples, Owing to its large surface area and high adsorption capacity, multi-walled carbon nanotube （MWCNT） was chosen as the adsorbent for the extraction of auxins from plant samples. In this study, two important auxins were selected as model analytes, namely indole-3- butyric acid （IBA） and 1-naphthylacetic acid （NAA）. They could be extracted and concentrated due to their π-π stacking interactions with MWCNT. Then HPLC-UV was introduced to detect IBA and NAA after sample pretreatment. Factors that may affect the enrichment efficiency were investigated and optimized. Comparative studies showed that MWCNT was superior to CI 8 for the extraction of the two analytes. Validation experiments showed that the optimized method had good linearity （0.9998 and 0.9960）, high recovery （81.4%-85.4%）, and low detection limits （0.0030 mg/L and 0.0012 mg/L）. The results indicated that the novel method had advantages of convenience, good sensitivity, high efficiency, and it was feasible for the determination of auxins in plant samples.

1-丁基-3-甲基咪唑磷钨酸盐的制备及其对酯化反应的催化性能

以溴化1-丁基-3-甲基咪唑盐([bmim]Br)和磷钨酸(H3PW12O40)为原料制备了1-丁基-3-甲基咪唑磷钨酸盐([bmim]3PW12O40)催化剂,并用傅里叶变换红外光谱、热重分析-差示扫描量热法、正丁胺电位滴定及元素分析等技术对催化剂进行了表征,考察了催化剂对乙醇与乙酸酯化合成乙酸乙酯反应的催化活性.结果表明,[bmim]3PW12O40中有3个结晶水,并保持有H3PW12O40的Keggin结构和酸强度,[bmim]3PW12O40的酸量明显少于H3PW12O40的酸量.在乙醇与乙酸酯化合成乙酸乙酯反应中,[bmim]3PW12O40催化剂具有较高的催化活性和较好的重复使用性能.

5-氟尿嘧啶-1-基乙酸合成方法的改进

以氯乙酸和5-氟尿嘧啶为原料合成了标题化合物,产率81%,产品纯度99.57%。

微波促进下2-芳氧甲基苯并咪唑-1-乙酸衍生物的合成及生物活性研究

采用微波辐射和相转移催化技术,在K2CO3存在下利用PEG-400作相转移催化剂,经过N-烷化、水解和酸化等步骤合成了10种尚未见文献报道的2-芳氧甲基苯并咪唑-1-乙酸衍生物.经元素分析,FT-IR,1H NMR和13C NMR确证了其结构.生物活性实验结果表明该系列化合物对小麦幼苗根系和芽的生长均有明显的调节活性.

2-(1-咪唑基)-乙酸的合成工艺改进

以咪唑和氯乙酸乙酯为原料,乙腈为溶剂,三乙胺为缚酸剂合成了2-(1-咪唑基)-乙酸乙酯,再经水解得到2-(1-咪唑基)-乙酸,并经1H NMR,13C NMR,IR确证了结构。考察了反应时间及三乙胺用量对反应收率的影响,结果表明,在反应时间4 h,n(三乙胺);n(咪唑)=1．3:1条件下,产物总收率为60．5％,含量(质量分数)大于98．6%。

原位法制备H_3PW_(12)O_(40)/SiO_2催化剂及其催化性能

用原位合成法制备了不同固载量的磷钨酸催化剂H3PW12O40/SiO2作催化剂,通过FT-IR、XRD等手段对催化剂进行表征。结果表明,在煅烧温度为200℃,活化时间为4h,H3PW12O40负载量为20%时,原位法制备的催化剂在载体孔壁中高度分散的磷钨酸仍然保持了Keggin特征结构,且与SiO2材料的表面羟基存在一定的化学相互作用。对苯甲醛1,2-丙二醇缩醛合成的催化性能研究表明,固定苯甲醛用量为0.2mol,在n(苯甲醛)∶n(1,2-丙二醇)=1∶1.5,催化剂的用量占反应物料总质量1.0%,环己烷用量为8mL,反应时间为60min条件下,产品收率可达88.5%。催化剂使用5次以后,催化活性仍然保持在接近51.7%。

阳离子交换树脂催化1-丁烯合成醋酸仲丁酯

以强酸性阳离子交换树脂为催化剂催化1-丁烯与醋酸合成醋酸仲丁酯。考察了原料烯酸配比、反应压力、反应时间、反应温度、催化剂用量等因素对醋酸转化率的影响,结果表明:在原料烯酸比为2.0∶1,反应压力5.5MPa,反应时间11h,反应温度120℃,催化剂用量为醋酸质量的10%的条件下,醋酸转化率为92.4%～95%。气相色谱-质谱分析表明,醋酸仲丁酯的选择性为92%。该催化剂对1-丁烯与醋酸的酯化反应有良好的催化活性和选择性,且催化剂稳定性良好。

5-硝基苯并三唑-1-乙酸镝与1,10-邻菲啰啉配合物的合成和性质研究

采用溶液合成法,合成了标题化合物,通过元素分析、摩尔电导率、紫外光谱、红外光谱和热分析法确定配合物的组成为Dy(L)3phen.1.5H2O(HL=5-硝基苯并三唑-1-乙酸;phen=1,10-邻菲啰啉)。荧光光谱表明:镝配合物的荧光强度较强,说明配体5-硝基苯并三唑-1-乙酸和1,10-邻菲啰啉是很好的敏化剂。

钐(Ⅲ)-1H-苯并三唑-1-乙酸-1,10-邻菲啰啉配合物的水热合成、表征及晶体结构

标题配合物[Sm(L)3(Phen)H2O]2(HL=1H-苯并三唑-1-乙酸;Phen=1,10-邻菲啰啉)是由SmCl3.6H2O、1H-苯并三唑-1-乙酸和1,10-邻菲啰啉经水热反应得到。采用元素分析、摩尔电导、红外光谱、热分析、X-射线单晶衍射手段对其进行表征。晶体结构表明:晶体属于单斜晶系,空间群为P2(1)/n。晶胞参数为:a=13.887 9(6),b=12.973 4(5),c=21.713 7(9),α=90°,β=100.820(2)°,γ=90°。V=3 842.7(3)3,Z=2,Dc=1.602 Mg.m-3,μ=1.600 mm-1,F(000)=1 860。配合物中每个Sm(Ⅲ)与来自4个配体L的6个氧原子、1个配位水分子的氧原子和1个邻菲啰啉分子中的2个氮原子配位,形成一个9配位的单帽四方反棱柱配位多面体。

十二烷基磺酸铁催化合成苯甲醛1,2-丙二醇缩醛

以十二烷基磺酸铁为催化剂,苯甲醛、1,2-丙二醇为原料,研究合成了苯甲醛1,2-丙二醇缩醛。分别考察了反应时间、催化剂用量、原料配比、带水剂用量等条件对产物收率的影响。结果表明:十二烷基磺酸铁是合成苯甲醛1,2-丙二醇缩醛的良好催化剂,在n(苯甲醛):n(1,2-丙二醇)=1.0∶1.3,催化剂用量为反应物料总质量的1%,带水剂环己烷为7.5 mL(苯甲醛为1 mol时),反应时间90 min的优化条件下,苯甲醛1,2-丙二醇缩醛的收率可达79.5%。

1-丁基-3-甲基咪唑磷钨酸盐催化合成乙酸乙酯的研究

以溴化2-丁基-3～甲基咪唑盐和磷钨酸为原料制备的具有Keggin结构的1-丁基-3-甲基咪唑磷钨酸盐[（Bmlm）3PW12O40·3H2O3催化合成乙酸乙酯，考察了1-丁基-3-甲基咪唑磷钨酸盐的催化活性以及带水剂、催化剂用量、酸醇摩尔比、反应时间对乙酸乙酯合成收率的影响．实验结果表明，具有Keggin结构的1-丁基-3-甲基咪唑磷钨酸盐[（Bmlm）3PW12O40·3H2O3呈现出较高的催化活性和较好的重复使用性能。优化的合成乙酸乙酯的工艺条件为：酸醇摩尔比为3：1，1-丁基-3-甲基咪唑磷钨酸盐催化剂用量为3g（以每摩尔乙醇计），带水剂甲苯的用量为4.0mL（以每摩尔乙醇计），回流反应时间为5。5h．在此所选择的条件下，合成乙酸乙酯的收率达99．68％～99.91％．