The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the...The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the BEAR1-RNAi and bear1 CRISPR mutants had accelerated seedling growth.Histological section of leaves showed accelerated development of lacuna and vascular bundles in bear1 mutant.GUS staining revealed that BEAR1 was highly expressed in coleoptiles and leaves at seedling stage.Expression analysis of gibberellin(GA)biosynthesis and metabolic genes and content determination of active GAs indicated that the expression of GA biosynthesis genes,especially OsKS4 and OsCPS2,were upregulated and the GAs content were significantly increased in bear1,which correlated with the seedling phenotype of bear1 mutant.Molecular and biochemical assays revealed that BEAR1 directly binds to the promoter of OsKS4,thereby repressing its expression.Haplotypes analysis showed clear differentiation in indica and japonica rice varieties,and a strong correlation with seedling height.These findings provide novel insights into the regulation of seedling growth in rice.展开更多
Dear editor,I am Dr.Jie Peng,from the Department of Ophthalmology,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China.I write to present a case report of a novel in-frame del...Dear editor,I am Dr.Jie Peng,from the Department of Ophthalmology,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China.I write to present a case report of a novel in-frame deletion mutation c.17779del TAC of neurofibromatosis type 1 in a Chinese boy with bilateral blindness.Neurofibromatosis type 1(NF1;OMIM#162200),an autosomal dominant disease,is caused by mutations in the NF1gene.The incidence of this disease is around 1 in 3500展开更多
The effects of different basic seedlings, N application rates and paclobutrazol measures on the development and yield of Tongyoujing 1 population were studied through the D-saturated optimum regression design tests. T...The effects of different basic seedlings, N application rates and paclobutrazol measures on the development and yield of Tongyoujing 1 population were studied through the D-saturated optimum regression design tests. The results showed thatthe effects of various factors on yield under the experimental conditions were in order of N application rate basic seedlings paclobutrazol dosage. The increases of basic seedlings and N application rate are beneficial to the increases of population quantity and tiller density, and could improve number of panicles per unit area and increase the population leaf area and accumulated amount of dry matter.However, too-high basic seedlings and N application rate would reduce reproductive tiller percentage and economic coefficient, and prolong growth duration. Proper basic seedlings could coordinate the correlation between tiller density and productive tiller percentage, ensure higher leaf area and dry matter accumulation at filling stage,and improve yield of Tongyoujing 1. According to the multivariate nonlinear regression analysis of yield with various factors, Tongyoujing 1 could reach its maximum yield potential of 12 606.0 kg/hm2 under the basic seedlings of 55.2×104/hm2, N application rate at 339.2 kg/hm2, and paclobutrazol dosage of 97.9 g/hm2. The comprehensive agricultural measures for a yield higher than 11 250 kg/hm2were： basic seedlings 52.6 ×10^4-60.1 ×10^4/hm2, N application rate 328.0-356.1 kg/hm2, and paclobutrazol dosage 87.6-104.6 g/hm2.展开更多
基金supported by the National Natural Science Foundation of China(32071932)Scientific Research Foundation for Scholars of HZNU(2019QDL015)the Natural Science Foundation of Zhejiang province(LQ22C130001)。
文摘The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the BEAR1-RNAi and bear1 CRISPR mutants had accelerated seedling growth.Histological section of leaves showed accelerated development of lacuna and vascular bundles in bear1 mutant.GUS staining revealed that BEAR1 was highly expressed in coleoptiles and leaves at seedling stage.Expression analysis of gibberellin(GA)biosynthesis and metabolic genes and content determination of active GAs indicated that the expression of GA biosynthesis genes,especially OsKS4 and OsCPS2,were upregulated and the GAs content were significantly increased in bear1,which correlated with the seedling phenotype of bear1 mutant.Molecular and biochemical assays revealed that BEAR1 directly binds to the promoter of OsKS4,thereby repressing its expression.Haplotypes analysis showed clear differentiation in indica and japonica rice varieties,and a strong correlation with seedling height.These findings provide novel insights into the regulation of seedling growth in rice.
文摘Dear editor,I am Dr.Jie Peng,from the Department of Ophthalmology,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China.I write to present a case report of a novel in-frame deletion mutation c.17779del TAC of neurofibromatosis type 1 in a Chinese boy with bilateral blindness.Neurofibromatosis type 1(NF1;OMIM#162200),an autosomal dominant disease,is caused by mutations in the NF1gene.The incidence of this disease is around 1 in 3500
基金Supported by Jiangsu Sanxin Agricultural Innovation Project(SXGC[2014]188)~~
文摘The effects of different basic seedlings, N application rates and paclobutrazol measures on the development and yield of Tongyoujing 1 population were studied through the D-saturated optimum regression design tests. The results showed thatthe effects of various factors on yield under the experimental conditions were in order of N application rate basic seedlings paclobutrazol dosage. The increases of basic seedlings and N application rate are beneficial to the increases of population quantity and tiller density, and could improve number of panicles per unit area and increase the population leaf area and accumulated amount of dry matter.However, too-high basic seedlings and N application rate would reduce reproductive tiller percentage and economic coefficient, and prolong growth duration. Proper basic seedlings could coordinate the correlation between tiller density and productive tiller percentage, ensure higher leaf area and dry matter accumulation at filling stage,and improve yield of Tongyoujing 1. According to the multivariate nonlinear regression analysis of yield with various factors, Tongyoujing 1 could reach its maximum yield potential of 12 606.0 kg/hm2 under the basic seedlings of 55.2×104/hm2, N application rate at 339.2 kg/hm2, and paclobutrazol dosage of 97.9 g/hm2. The comprehensive agricultural measures for a yield higher than 11 250 kg/hm2were： basic seedlings 52.6 ×10^4-60.1 ×10^4/hm2, N application rate 328.0-356.1 kg/hm2, and paclobutrazol dosage 87.6-104.6 g/hm2.
