AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA...AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA sequences as well as complete clinical data of SA and adjacent normal tissues from patients. Weighted gene co-expression network analysis （WGCNA） was used to investigate the meaningful module along with hub genes. Expression of hub genes was analyzed in 362 paraffin-embedded SA biopsy tissues by immunohistochemical staining. Patients were classified into two groups （according to expression of hub genes）： Weak expression and over-expression groups. Correlation of biomarkers with clinicopathological factors indicated patient survival.RESULTS Whole genome expression level screening identified 6,231 differentially expressed genes. Twenty-four co- expressed gene modules were identified using WGCNA. Pearson＇s correlation analysis showed that the tan module was the most relevant to tumor stage （r = 0.24, P = 7 × 10 -6）. In addition, we detected sorting nexin （SNX）10 as the hub gene of the tan module. SNX10 expression was linked to T category （P = 0.042, x2= 8.708）, N category （P = 0.000, x2= 18.778）, TNM stage （P = 0.001, x2 = 16.744） as well as tumor differentiation （P = 0.000,x2= 251.930）. Patients with high SNX10 expression tended to have longer diseasefree survival （DFS; 44.97 mo vs 33.85 mo, P = 0.000） as well as overall survival （OS; 49.95 vs 40.84 mo, P = 0.000） in univariate analysis. Multivariate analysis showed that dismal prognosis could be precisely predicted clinicopathologically using SNX10 [DFS： P = 0.014, hazard ratio （HR） = 0.698, 95% confidence interval （CI）： 0.524-0.930, OS： P = 0.017, HR = 0.704, 95%CI： 0.528-0.940].CONCLUSION This study provides a new technique for screening prognostic biomarkers of SA. Weak expression of SNX10 is linked to poor prognosis, and is a suitable prognostic biomarker of SA.展开更多
Aim:Long-term survival after hepatocellular cancer(HCC)is difficult to achieve likely related to recurrence.This study aimed to identify factors that were predictive of 10-year survival after the diagnosis of HCC.Meth...Aim:Long-term survival after hepatocellular cancer(HCC)is difficult to achieve likely related to recurrence.This study aimed to identify factors that were predictive of 10-year survival after the diagnosis of HCC.Methods:In a prospectively collected database of 1374 HCC cases(1993-2019),we identified 70 patients who survived over 10 years regardless of treatment.We then identified 164 patients in the entire cohort who either had liver resection or transplant,and died before 10 years.Demographics,tumor characteristics,treatment,recurrence and treatment of recurrence were compared.Results:Of the 10-year survivors,36 underwent transplant,27 had liver resection and 7 patients had only locoregional therapy.Compared to the non-survivors,the 10-year survivors were younger and had fewer comorbidities or recurrence,smaller tumor size,lower AST,ALT,AFP,platelets,neutrophil-to-lymphocyte ratio.Multivariate analysis showed only age and diabetes to be negative predictors.Recurrence occurred in 24 survivors(34.3%)with mean time to recurrence with standard deviation 57.1±42.6 months compared to 80 non-survivors(48.7%)with mean time to recurrence of 15.3±14.8 months.For hepatic resection,10-year survivors had longer time to recurrence compared to non-survivors(median:31.3 months).Conclusion:Long-term survivors mostly occur after resection or transplant,but 10%of our cohort survived 10 years with only locoregional therapy.Underlying health status maybe an important predictor of 10-year survival for ;patients receiving liver resections.Recurrence of HCC occurs in both 10-year survivors and non-survivors,but later recurrence with aggressive treatment of the recurrence may allow for 10-year survival.展开更多
文摘AIM TO detect significant clusters of co-expressed genes associated with tumorigenesis that might help to predict stomach adenocarcinoma （SA） prognosis.METHODS The Cancer Genome Atlas database was used to obtain RNA sequences as well as complete clinical data of SA and adjacent normal tissues from patients. Weighted gene co-expression network analysis （WGCNA） was used to investigate the meaningful module along with hub genes. Expression of hub genes was analyzed in 362 paraffin-embedded SA biopsy tissues by immunohistochemical staining. Patients were classified into two groups （according to expression of hub genes）： Weak expression and over-expression groups. Correlation of biomarkers with clinicopathological factors indicated patient survival.RESULTS Whole genome expression level screening identified 6,231 differentially expressed genes. Twenty-four co- expressed gene modules were identified using WGCNA. Pearson＇s correlation analysis showed that the tan module was the most relevant to tumor stage （r = 0.24, P = 7 × 10 -6）. In addition, we detected sorting nexin （SNX）10 as the hub gene of the tan module. SNX10 expression was linked to T category （P = 0.042, x2= 8.708）, N category （P = 0.000, x2= 18.778）, TNM stage （P = 0.001, x2 = 16.744） as well as tumor differentiation （P = 0.000,x2= 251.930）. Patients with high SNX10 expression tended to have longer diseasefree survival （DFS; 44.97 mo vs 33.85 mo, P = 0.000） as well as overall survival （OS; 49.95 vs 40.84 mo, P = 0.000） in univariate analysis. Multivariate analysis showed that dismal prognosis could be precisely predicted clinicopathologically using SNX10 [DFS： P = 0.014, hazard ratio （HR） = 0.698, 95% confidence interval （CI）： 0.524-0.930, OS： P = 0.017, HR = 0.704, 95%CI： 0.528-0.940].CONCLUSION This study provides a new technique for screening prognostic biomarkers of SA. Weak expression of SNX10 is linked to poor prognosis, and is a suitable prognostic biomarker of SA.
文摘Aim:Long-term survival after hepatocellular cancer(HCC)is difficult to achieve likely related to recurrence.This study aimed to identify factors that were predictive of 10-year survival after the diagnosis of HCC.Methods:In a prospectively collected database of 1374 HCC cases(1993-2019),we identified 70 patients who survived over 10 years regardless of treatment.We then identified 164 patients in the entire cohort who either had liver resection or transplant,and died before 10 years.Demographics,tumor characteristics,treatment,recurrence and treatment of recurrence were compared.Results:Of the 10-year survivors,36 underwent transplant,27 had liver resection and 7 patients had only locoregional therapy.Compared to the non-survivors,the 10-year survivors were younger and had fewer comorbidities or recurrence,smaller tumor size,lower AST,ALT,AFP,platelets,neutrophil-to-lymphocyte ratio.Multivariate analysis showed only age and diabetes to be negative predictors.Recurrence occurred in 24 survivors(34.3%)with mean time to recurrence with standard deviation 57.1±42.6 months compared to 80 non-survivors(48.7%)with mean time to recurrence of 15.3±14.8 months.For hepatic resection,10-year survivors had longer time to recurrence compared to non-survivors(median:31.3 months).Conclusion:Long-term survivors mostly occur after resection or transplant,but 10%of our cohort survived 10 years with only locoregional therapy.Underlying health status maybe an important predictor of 10-year survival for ;patients receiving liver resections.Recurrence of HCC occurs in both 10-year survivors and non-survivors,but later recurrence with aggressive treatment of the recurrence may allow for 10-year survival.
文摘AIM: To conduct a meta-analysis to evaluate the prognostic role of hypoxia inducible factor-1α (HIF-1α) expression in gastric cancer.
