To investigate effects of Zn on Cd uptake by spring wheat (Triticum aestivum, L.) in solution culture, long-time hydroponic experiment (1 month) (Experiment 1) and short-time Cd isotope (109Cd) tracing experiment (24 ...To investigate effects of Zn on Cd uptake by spring wheat (Triticum aestivum, L.) in solution culture, long-time hydroponic experiment (1 month) (Experiment 1) and short-time Cd isotope (109Cd) tracing experiment (24 h) (Experiment 2) were conducted. In Experiment 1, spring wheat (cv. Brookton) was grown in nutrient solution at uniform cadmium concentration of 20μ mol/L and 10 zinc concentrations (0, 1, 5, 10, 20, 100, 200, 500, 1000, 2000 μ mol/L). In Experiment 2, spring wheat seedlings,pre-cultivated in complete nutrient solution, were treated with 109Cd of uniform activity and the same series of Zn concentrations as those in Experiment 1 for 24 h. Cd concentrations in shoots and roots in Experiment 1 increased marginally but not consistently with Zn increasing at Zn rates of 1～200 μmol/L, and then decreased significantly at high rates (＞200 μ mol/L). In Experiment 2, the response of 109Cd activities in shoots and roots to increasing Zn was greatly similar to the response of Cd concentrations to Zn increasing in Experiment 1. The results of the two experiments indicated that the short-time and long-time exposure of spring wheat to Zn had similar effects on Cd accumulation.展开更多
A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of ...A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of expressed prostatic fluid were obtained from 51 men(mean age 51 years,range 18–82 years) with apparently normal prostates using standard rectal massage procedure.Mean values(M ± SEL) for concentration of trace elements(mg L^(-1)) in human prostate fluid were:Fe 9.04 ±1.21,Zn 573 ± 35,Br 3.58 ± 0.59,Rb 1.10 ± 0.08,and Sr B 0.76.It was shown that the results of trace element analysis in the micro-samples(20 l L) are sufficiently representative for assessment of the Fe,Zn,Br,and Rb concentration in the prostate fluid.The facility for109 Cd radionuclide-induced EDXRF is comparatively compact and can be located in close proximity to the site of carrying out the massage procedure.The means of Zn and Rb concentration obtained for prostate fluid agree well with median of reported means.For the first time,the Fe and Br concentrations,as well as the upper limit of the Sr concentration,were determined in the human prostate fluid.展开更多
Transforming growth factor（TGF）-β signaling plays an important role in the pathogenesis of psoriasis. CD109, a novel TGF-β co-receptor, which inhibits TGF-β signaling by enhancing Smad7-dependent degradation of T...Transforming growth factor（TGF）-β signaling plays an important role in the pathogenesis of psoriasis. CD109, a novel TGF-β co-receptor, which inhibits TGF-β signaling by enhancing Smad7-dependent degradation of TGF-β type Ⅰ receptor（TGF-β RⅠ）, is abnormally expressed in psoriasis. To date, the expression of Smad7 and the correlation between CD109 and Smad7 expression in psoriasis have not been fully elucidated. This study was designed to investigate the expression and the correlation of CD109 and TGF-β signaling associated proteins in psoriasis and their roles in the pathogenesis of psoriasis. Thirty-two psoriasis specimens were subjected to immunohistochemical staining for CD109, Smad7, TGF-β RⅠ and Ki67. Ten normal skin（NS） specimens served as controls. The positive expression rate（% positive cells） of Smad7 and Ki67 in psoriasis was significantly higher than in NS（62.6%±19.9% vs. 17.2%±4.4%, and 50.7%±14.3% vs. 19.5%±3.2%, respectively, P〈0.001）, and the expression levels of CD109 and TGF-β RⅠ were reduced significantly in psoriasis as compared with NS（8.1%±6.7% vs. 35.8%±6.7% and 27.3%±3.4% vs. 3.0%±3.4%, respectively, P〈0.001）. There were significantly negative correlations between CD109 and Smad7（r=-0.831, P〈0.01）. These findings indicated that CD109 might play a certain role in the pathogenesis of psoriasis. Lower expression of CD109 and TGF-β RⅠ was highly correlated with higher expression of Smad7 and Ki67, suggesting that CD109 may induce the pathogenesis of psoriasis through Smad7-mediated degradation of TGF-β RⅠ, and lead to the termination of TGF-β signaling.展开更多
TALYS code was used to calculate excitation functions for proton induced onn atAg,natCd and 113In,deuteron induced onn atAg and alpha induced on natPd that lead to produce 109Cd radioisotope using low and medium energ...TALYS code was used to calculate excitation functions for proton induced onn atAg,natCd and 113In,deuteron induced onn atAg and alpha induced on natPd that lead to produce 109Cd radioisotope using low and medium energy accelerators;calculates was performed out up to 50 MeV.Recommended thickness of the targets according to SRIM code was premeditated.Theoretical integral yields for any reaction were computed.TALYS 1.0 code predicts that production of a few curries of 109Cd is feasible using a target of high isotopically enriched 110Cd,proton energy of 15 to 27 MeV,however high cost of the enriched target seems to be not a practical economic alternative to produce1 09Cd.The Ag+p and Ag+d processes can be an efficient route and economic for the production of millicurries of the radioisotope with incident beam energy less 15 MeV using low energy accelerator.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 40335046) and the "Recruiting Outstanding Overseas Chinese Scientists" Scheme of the Chinese Academy of Sciences, China
文摘To investigate effects of Zn on Cd uptake by spring wheat (Triticum aestivum, L.) in solution culture, long-time hydroponic experiment (1 month) (Experiment 1) and short-time Cd isotope (109Cd) tracing experiment (24 h) (Experiment 2) were conducted. In Experiment 1, spring wheat (cv. Brookton) was grown in nutrient solution at uniform cadmium concentration of 20μ mol/L and 10 zinc concentrations (0, 1, 5, 10, 20, 100, 200, 500, 1000, 2000 μ mol/L). In Experiment 2, spring wheat seedlings,pre-cultivated in complete nutrient solution, were treated with 109Cd of uniform activity and the same series of Zn concentrations as those in Experiment 1 for 24 h. Cd concentrations in shoots and roots in Experiment 1 increased marginally but not consistently with Zn increasing at Zn rates of 1～200 μmol/L, and then decreased significantly at high rates (＞200 μ mol/L). In Experiment 2, the response of 109Cd activities in shoots and roots to increasing Zn was greatly similar to the response of Cd concentrations to Zn increasing in Experiment 1. The results of the two experiments indicated that the short-time and long-time exposure of spring wheat to Zn had similar effects on Cd accumulation.
文摘A facility and method for109 Cd radionuclide-induced energy-dispersive X-ray fluorescent(EDXRF) were developed to determine the Fe,Zn,Br,Rb,and Sr concentrations in the specimens of human prostatic fluid.Specimens of expressed prostatic fluid were obtained from 51 men(mean age 51 years,range 18–82 years) with apparently normal prostates using standard rectal massage procedure.Mean values(M ± SEL) for concentration of trace elements(mg L^(-1)) in human prostate fluid were:Fe 9.04 ±1.21,Zn 573 ± 35,Br 3.58 ± 0.59,Rb 1.10 ± 0.08,and Sr B 0.76.It was shown that the results of trace element analysis in the micro-samples(20 l L) are sufficiently representative for assessment of the Fe,Zn,Br,and Rb concentration in the prostate fluid.The facility for109 Cd radionuclide-induced EDXRF is comparatively compact and can be located in close proximity to the site of carrying out the massage procedure.The means of Zn and Rb concentration obtained for prostate fluid agree well with median of reported means.For the first time,the Fe and Br concentrations,as well as the upper limit of the Sr concentration,were determined in the human prostate fluid.
基金supported by the National Natural Science Foundation of China(No.81101191and No.30972654)
文摘Transforming growth factor（TGF）-β signaling plays an important role in the pathogenesis of psoriasis. CD109, a novel TGF-β co-receptor, which inhibits TGF-β signaling by enhancing Smad7-dependent degradation of TGF-β type Ⅰ receptor（TGF-β RⅠ）, is abnormally expressed in psoriasis. To date, the expression of Smad7 and the correlation between CD109 and Smad7 expression in psoriasis have not been fully elucidated. This study was designed to investigate the expression and the correlation of CD109 and TGF-β signaling associated proteins in psoriasis and their roles in the pathogenesis of psoriasis. Thirty-two psoriasis specimens were subjected to immunohistochemical staining for CD109, Smad7, TGF-β RⅠ and Ki67. Ten normal skin（NS） specimens served as controls. The positive expression rate（% positive cells） of Smad7 and Ki67 in psoriasis was significantly higher than in NS（62.6%±19.9% vs. 17.2%±4.4%, and 50.7%±14.3% vs. 19.5%±3.2%, respectively, P〈0.001）, and the expression levels of CD109 and TGF-β RⅠ were reduced significantly in psoriasis as compared with NS（8.1%±6.7% vs. 35.8%±6.7% and 27.3%±3.4% vs. 3.0%±3.4%, respectively, P〈0.001）. There were significantly negative correlations between CD109 and Smad7（r=-0.831, P〈0.01）. These findings indicated that CD109 might play a certain role in the pathogenesis of psoriasis. Lower expression of CD109 and TGF-β RⅠ was highly correlated with higher expression of Smad7 and Ki67, suggesting that CD109 may induce the pathogenesis of psoriasis through Smad7-mediated degradation of TGF-β RⅠ, and lead to the termination of TGF-β signaling.
文摘TALYS code was used to calculate excitation functions for proton induced onn atAg,natCd and 113In,deuteron induced onn atAg and alpha induced on natPd that lead to produce 109Cd radioisotope using low and medium energy accelerators;calculates was performed out up to 50 MeV.Recommended thickness of the targets according to SRIM code was premeditated.Theoretical integral yields for any reaction were computed.TALYS 1.0 code predicts that production of a few curries of 109Cd is feasible using a target of high isotopically enriched 110Cd,proton energy of 15 to 27 MeV,however high cost of the enriched target seems to be not a practical economic alternative to produce1 09Cd.The Ag+p and Ag+d processes can be an efficient route and economic for the production of millicurries of the radioisotope with incident beam energy less 15 MeV using low energy accelerator.
