Laboratory mutants of Sclerotinia sclerotiorum(Lib)de Bary,resistant to boscalid,have been extensively characterized.However,the resistance situation in the lettuce field remains largely elusive.In this study,among th...Laboratory mutants of Sclerotinia sclerotiorum(Lib)de Bary,resistant to boscalid,have been extensively characterized.However,the resistance situation in the lettuce field remains largely elusive.In this study,among the 172 S.sclerotiorum isolates collected from asparagus lettuce field in Jiangsu Province,China,132 isolates(76.74%)exhibited low-level resistance to boscalid(Bos^(LR)),with a discriminatory dose of 5μg mL-1.In comparison to the boscalid-sensitive(BosS)isolates,most Bos^(LR)isolates demonstrated a slightly superior biological fitness,as evidenced by data on mycelial growth,sclerotium production and pathogenicity.Moreover,most Bos^(LR)isolates showed comparable levels of oxalic acid(OA)accumulation,increased exopolysaccharide(EPS)content and reduced membrane permeability when compared to the BosS isolates.Nevertheless,their responses to distinct stress factors diverged significantly.Furthermore,the effectiveness of boscalid in controlling Bos^(LR)isolates on radish was diminished compared to its efficacy on BosS isolates.Genetic mutations were identified in the SDH genes of Bos^(LR)isolates,revealing the existence of three resistant genotypes:I(^(A11V)at SDHB,SDHB^(A11V)),II(^(Q38R)at SDHC,SDHC^(Q38R))and III(SDHB^(A11V)+SDHC^(Q38R)).Importantly,no cross-resistance was observed between boscalid and other fungicides such as thifluzamide,pydiflumetofen,fluazinam,or tebuconazole.Our molecular docking analysis indicated that the docking total score(DTS)of the type I resistant isolates(1.3993)was lower than that of the sensitive isolates(1.7499),implying a reduced affinity between SDHB and boscalid as a potential mechanism underlying the boscalid resistance in S.sclerotiorum.These findings contribute to an enhanced comprehension of boscalid’s mode of action and furnish valuable insights into the management of boscalid resistance.展开更多
S7-1200PLC及DP主站模块CM1243-5在XJZ上悬自动卸料离心机上的应用,为我们又提供了一种新的控制方式,弥补了中档配置的空缺,使我们的产品梯度更优,同时也迎合了部分客户对产品高自动化、高稳定性、价格适中的要求。软件编程采用直观、...S7-1200PLC及DP主站模块CM1243-5在XJZ上悬自动卸料离心机上的应用,为我们又提供了一种新的控制方式,弥补了中档配置的空缺,使我们的产品梯度更优,同时也迎合了部分客户对产品高自动化、高稳定性、价格适中的要求。软件编程采用直观、高效、可靠的IEMENS TIA Portal V11软件,新的编程软件使人耳目一新,编程工作方便而快捷。展开更多
基金supported by the Jiangsu Provincial Key Research and Development, China (BE2021361)the Jiangsu Agriculture Science and Technology Innovation Fund ((CX(21)2037 and CX(22)3072))the National Natural Science Foundation of China (31672065)。
文摘Laboratory mutants of Sclerotinia sclerotiorum(Lib)de Bary,resistant to boscalid,have been extensively characterized.However,the resistance situation in the lettuce field remains largely elusive.In this study,among the 172 S.sclerotiorum isolates collected from asparagus lettuce field in Jiangsu Province,China,132 isolates(76.74%)exhibited low-level resistance to boscalid(Bos^(LR)),with a discriminatory dose of 5μg mL-1.In comparison to the boscalid-sensitive(BosS)isolates,most Bos^(LR)isolates demonstrated a slightly superior biological fitness,as evidenced by data on mycelial growth,sclerotium production and pathogenicity.Moreover,most Bos^(LR)isolates showed comparable levels of oxalic acid(OA)accumulation,increased exopolysaccharide(EPS)content and reduced membrane permeability when compared to the BosS isolates.Nevertheless,their responses to distinct stress factors diverged significantly.Furthermore,the effectiveness of boscalid in controlling Bos^(LR)isolates on radish was diminished compared to its efficacy on BosS isolates.Genetic mutations were identified in the SDH genes of Bos^(LR)isolates,revealing the existence of three resistant genotypes:I(^(A11V)at SDHB,SDHB^(A11V)),II(^(Q38R)at SDHC,SDHC^(Q38R))and III(SDHB^(A11V)+SDHC^(Q38R)).Importantly,no cross-resistance was observed between boscalid and other fungicides such as thifluzamide,pydiflumetofen,fluazinam,or tebuconazole.Our molecular docking analysis indicated that the docking total score(DTS)of the type I resistant isolates(1.3993)was lower than that of the sensitive isolates(1.7499),implying a reduced affinity between SDHB and boscalid as a potential mechanism underlying the boscalid resistance in S.sclerotiorum.These findings contribute to an enhanced comprehension of boscalid’s mode of action and furnish valuable insights into the management of boscalid resistance.
基金Supported by the National Natural Science Foundation of China(No.21477050,No.21522603,No.21706101)the Henry Fok Education Foundation(No.141068)+2 种基金the Six Talents Peak Project in Jiangsu Province(No.XCL-025)the Chinese-German Cooperation Research Project(No.GZ1091)the China Postdoctoral Foundation(No.2017M611731)~~
文摘S7-1200PLC及DP主站模块CM1243-5在XJZ上悬自动卸料离心机上的应用,为我们又提供了一种新的控制方式,弥补了中档配置的空缺,使我们的产品梯度更优,同时也迎合了部分客户对产品高自动化、高稳定性、价格适中的要求。软件编程采用直观、高效、可靠的IEMENS TIA Portal V11软件,新的编程软件使人耳目一新,编程工作方便而快捷。
