We fabricate a novel cellulose acetate （CA） ultrafiltration membrane modified by block copolymer F127-b- PDMAEMA, which is synthesized using F127 and DMAEMA via the ARGET ATRP method. Compared to conven- tional ultr...We fabricate a novel cellulose acetate （CA） ultrafiltration membrane modified by block copolymer F127-b- PDMAEMA, which is synthesized using F127 and DMAEMA via the ARGET ATRP method. Compared to conven- tional ultrafiltration membranes, the incorporation of both F 127 and PDMAEMA can not only readily increase the hydrophilicity of the membrane, but also exhibit stimuli-responsiveness to temperature and pH. Fourier transform infrared spectroscopy （FT-IR）, nuclear magnetic resonance spectroscopy （NMR）, and gel permeation chromatog- raphy （GPC） are employed to analyze the structure of the F 127-b-PDMAEMA. The membrane properties are eval- uated via scanning electron microscope （SEM） imaging, porosity test, automatic target recognition Fourier trans- form infrared spectroscopy （ATR-FTIR）, water contact angle test and permeation test. The results indicate that the F 127-b-PDMAEMA is an excellent pore agent, which contributes to an enhancement of the membrane in sensitivity to temperature and pH. The modified membrane also exhibits lower water contact angle （64.5~）, which is attributed to the good anti-fouling performance and high water permeation.展开更多
The mucosal barrier remains a major barrier in the pulmonary drug delivery system,as mucociliary clearance in the airway accelerates the removal of inhaled nanoparticles(NPs).Herein,we designed and developed the inhal...The mucosal barrier remains a major barrier in the pulmonary drug delivery system,as mucociliary clearance in the airway accelerates the removal of inhaled nanoparticles(NPs).Herein,we designed and developed the inhalable Pluronic F127-modified silk fibroin NPs loading with quercetin(marked as QR-SF(PF127)NPs),aiming to solve the airway mucus barrier and improve the cancer therapeutic effect of QR.The PF127 coating on the SF NPs could attenuate the interaction between NPs and mucin proteins,thus facilitating the diffusion of SF(PF127)NPs in the mucus layer.The QR-SF(PF127)NPs had particle sizes of approximately 200 nm with negatively charged surfaces and showed constant drug release properties.Fluorescence recovery after photobleaching(FRAP)assay and transepithelial transport test showed that QR-SF(PF127)NPs exhibited superior mucus-penetrating ability in artificial mucus and monolayer Calu-3 cell model.Notably,a large amount of QR-SF(PF127)NPs distributed uniformly in the mice airway section,indicating the good retention of NPs in the respiratory tract.Themicemelanoma lungmetastasismodel was established,and the therapeutic effect of QR-SF(PF127)NPs was significantly improved in vivo.PF127-modified SF NPs may be a promising strategy to attenuate the interaction with mucin proteins and enhancemucus penetration efficiency in the pulmonary drug delivery system.展开更多
基金This work was financially supported by the Funda- mental Research Funds for the Central Universities of China （Nos. 3207045403, 3207045409）, National Natu- ral Science Foundation of China （Nos. 21576050, 51602052）, Jiangsu Provincial Natural Science Founda- tion of China （No. BK20150604） and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions （PAPD）.
文摘We fabricate a novel cellulose acetate （CA） ultrafiltration membrane modified by block copolymer F127-b- PDMAEMA, which is synthesized using F127 and DMAEMA via the ARGET ATRP method. Compared to conven- tional ultrafiltration membranes, the incorporation of both F 127 and PDMAEMA can not only readily increase the hydrophilicity of the membrane, but also exhibit stimuli-responsiveness to temperature and pH. Fourier transform infrared spectroscopy （FT-IR）, nuclear magnetic resonance spectroscopy （NMR）, and gel permeation chromatog- raphy （GPC） are employed to analyze the structure of the F 127-b-PDMAEMA. The membrane properties are eval- uated via scanning electron microscope （SEM） imaging, porosity test, automatic target recognition Fourier trans- form infrared spectroscopy （ATR-FTIR）, water contact angle test and permeation test. The results indicate that the F 127-b-PDMAEMA is an excellent pore agent, which contributes to an enhancement of the membrane in sensitivity to temperature and pH. The modified membrane also exhibits lower water contact angle （64.5~）, which is attributed to the good anti-fouling performance and high water permeation.
基金supported by the National Natural Science Foundation of China(No.52273123)the Graduate Scientific Research and Innovation Foundation of Chongqing,China(No.CYS21072)+1 种基金the Natural Science Foundation of Chongqing(cstc2021jcyj-msxmX0344,cstc2021jcyj-msxmX0342)the Open Research Project from State Key Laboratory of Silkworm GenomeBiology(No.SKLSGB-orp202010).
文摘The mucosal barrier remains a major barrier in the pulmonary drug delivery system,as mucociliary clearance in the airway accelerates the removal of inhaled nanoparticles(NPs).Herein,we designed and developed the inhalable Pluronic F127-modified silk fibroin NPs loading with quercetin(marked as QR-SF(PF127)NPs),aiming to solve the airway mucus barrier and improve the cancer therapeutic effect of QR.The PF127 coating on the SF NPs could attenuate the interaction between NPs and mucin proteins,thus facilitating the diffusion of SF(PF127)NPs in the mucus layer.The QR-SF(PF127)NPs had particle sizes of approximately 200 nm with negatively charged surfaces and showed constant drug release properties.Fluorescence recovery after photobleaching(FRAP)assay and transepithelial transport test showed that QR-SF(PF127)NPs exhibited superior mucus-penetrating ability in artificial mucus and monolayer Calu-3 cell model.Notably,a large amount of QR-SF(PF127)NPs distributed uniformly in the mice airway section,indicating the good retention of NPs in the respiratory tract.Themicemelanoma lungmetastasismodel was established,and the therapeutic effect of QR-SF(PF127)NPs was significantly improved in vivo.PF127-modified SF NPs may be a promising strategy to attenuate the interaction with mucin proteins and enhancemucus penetration efficiency in the pulmonary drug delivery system.
