Natural Nitrogen-15 Abundance of Ammonium Nitrogen and Fixed Ammonium in Soils

The present article deals with the natural nitrogen-15 abundance of ammonium nitrogen and fixed ammonium in different soils. Variations in the natural 15N abundance of ammonium nitrogen mineralized in soils under anaerobic incubation condition were related to soil pH. The δ 15N of mineralizable N in acid soils was lower but that in neutral and calcareous soils was higher compared with the δ 15N of total N in the soils. A variation tendency was also found in the δ 15N of amino-acid N in the hydrolysates of soils. The natural 15N abundance of fixed ammonium was higher than that of total N in most surface soils and other soil horizons, indicating that the increase of δ 15N in the soil horizons beneath subsurface horizon of some forest soils and acid paddy soils was related to the higher δ 15N value of fixed ammonium in the soil.

Natural Abundance of ^(15)N in Main N-Containing Chemical Fertilizers of China

As early as the 1950s, there had already been reports on the δ 15N value of some chemical fertilizers (Hoering, 1955). Since Kohl and his co-workers (1971) published the report that attempt was made to distinguish the respective contribution of soil nitrogen and fertilizer nitrogen to the source of nitrates in surface water according to the differ-

Study on Variation in ^(15)N Natural Abundance. Ⅰ. Characteristics of Variation in ^(25)N Natural Abundance of Forest Soils

INTRODUCTION A lot 0fwork has been done on the variation of natural ^(15)N abundance in soils andtheir N components (Chen et al., 1964). However, these reports mostly concerned thegeochemical mean of natural ^(15)N abundance, and only a little work has been done on thecharacteristics of variation of the natural ^(15)N abundance in different ecosystems and itsrelationship with the properties and conditions of soil, which is the aim of our recent re-

Temporal changes in nitrogen acquisition of Japanese black pine(Pinus thunbergii) associated with black locust(Robinia pseudoacacia)

The alien woody legume, black locust （Robinia pseudoaca-cia）, has invaded Japanese black pine （Pinus thunbergii） forests located in Japan’s coastal plain and hill regions where gaps are formed in pine forests after nematode infestation. Nitrogen fixation by legumes acceler-ates N cycling in forest ecosystems. We studied temporal change in the annual tree-ring resolution N stable isotope composition （δ15N, a per mil deviation of δ15N/14N ratio, relative to atmospheric N2δ15N=0‰） at two natural locations of Japanese black pine forest with black locust that differed in the time since black locust establishment （Shohnai in north-east and Kita-Kyushu in southwest Japan）. Analyzed tree-rings covered the period from 1990/1992 to 2009. N acquisition by Japanese black pine from black locust N input to the soil was evidenced by temporal shifting of N stable isotope composition on the annual pine tree rings. With pro-gressive development of the forest stand,δ15N values of earlier tree-ringsδ15N of -5‰） from black pine associated with black locust shifted to-wards values similar to those of black locustδ15N values nearly to-1‰）, which suggests acquisition of N by N2 fixation （Shohnai site）. In con-trast, in a forest where black locust had settled for two or three genera-tions, in a black pine stand （Kita-Kyushu site）, longer periods of N en-richment in the soil were reflected in the elevated tree-ringδ15N values of newly established black pine trees. Based on tree-ringδ15N data from the Shohnai site, we determined that about 10 years after black locust establishment, soil N had already been enriched by black locust N, this, in turn, contributed to N fertilization of surrounding trees in mixed stands.

Responses of plant^(15)N natural abundance and isotopic fractionation to N addition reflect the N status of a temperate steppe in China

Aims Elevated anthropogenic nitrogen(N)deposition could alter N status in temperate steppe.However,threshold observations of N status change from N limit to N saturation by far are not conclusive in these ecosystems.Research on the natural abundance of ^(15)N( δ^(15)N)could greatly help provide integrated information about ecosystem N status.The goal of this study was to investigate the suitability of measurements of δ^(15)N of major ecosystem N pools and several key species,plant ^(15)N fractionation,together with key vegetation and soil indicators in response to N fertilization as a tool to identify the N status in a temperate steppe in Inner Mongolia.Methods We carried out a N addition experiment during 2011-14 on a Stipa krylovii steppe in Inner Mongolia,Northern China.We investigated the response of several key N transformation processes,vegetation and soil properties to N addition.Aboveground biomass and below-ground biomass(BGB) δ^(15)N,root and foliar δ^(15)N of three dominant species(Artemisia frigida,S.krylovii and Leymus chinensis), δ^(15)N of soil total N and soil KCl-extractable NO_(3)^(−)-N were determined.The responses of isotope fractionation during plant N uptake and reallo-cation to N addition were also determined.Important Findings Our results suggest that the N addition rate of 5g N m^(−2) yr^(−1) could be regarded as threshold of early N saturation in this S.krylovii steppe as indicated by an increase in plant fraction-ation and a decrease in plant δ^(15)N.When N input rate is>10 g N m^(-2) yr^(-1),increased N deposition can lead to an apparent reduction in species richness and BGB as well as an increase in NO_(3)^(−)in extractable soil pools<30-cm soil profile.With N addition,S.krylovii and A.frigida undergo earlier N status shift from N limitation toward N excess compared with L.chinen-sis,contributing to L.chinensis out-competing other species.Overall,this study provides a better understanding of N status change in temperate steppe based on isotope evidence and several other functional variables and contributes to predicting the responses of temperate steppe to future global N deposition scenario.

Nitrogen uptake strategies of mature conifers in Northeastern China, illustrated by the ^(15)N natural abundance method

Background:Conifers partition different N forms from soil,including ammonium,nitrate,and dissolved organic N(DON),to sustain plant growth.Previous studies focused on inorganic N sources and specific amino acid forms using ^(15)N labelling,but knowledge of the contribution of DON to mature conifers’N uptake is still scarce.Here,we quantified the contribution of different N forms(DON vs.NH_(4)^(+)vs.NO_(3)^(−))to total N uptake,based on ^(15)N natural abundance of plant and soil available N,in four mature conifers(Pinus koraiensis,Pinus sylvestris,Picea koraiensis,and Larix olgensis).Results:DON contributed 31%,29%,28%,and 24%to total N uptake by Larix olgensis,Picea koraiensis,Pinus koraiensis,and Pinus sylvestris,respectively,whereas nitrate contributed 42 to 52%and ammonium contributed 19 to 29%of total N uptake for these four coniferous species.Conclusions:Our results suggested that all four conifers could take up a relatively large proportion of nitrate,while DON was also an important N source for the four conifers.Given that DON was the dominant N form in study soil,such uptake pattern of conifers could be an adaptive strategy for plants to compete for the limited available N sources from soil so as to promote conifer growth and maintain species coexistence.

Impact of Cutting a Clover Crop on the Nitrogen Supplied to Winter Wheat in an Intercropping System

The major function of clover in a winter wheat–white clover intercropping system is to supply nitrogen （N） for the wheat. A field experiment was conducted at Yucheng Comprehensive Station of the Chinese Academy of Sciences, to evaluate the effect of cutting white clover on N fixation and the transfer of fixed N to the associated winter wheat. A method of 15N natural abundance was used to determine the nitrogen dynamics in the intercropping system. The results showed that the amount of N transferred from the clover to the wheat, throughout the growing season, varied between 34.4 and 57.5 kg ha-1. Compared to leaving the clover standing, cutting the clover increased the amount of N that accumulated in the soil and also resulted in reduced N concentrations in the leaves and stems of the wheat. Using the cut clover as mulch between wheat rows led to decreased N concentrations in the wheat plants’ leaves and stems. The present study provides preliminary information on the amount of N transferred from clover to wheat in an intercropping system.

Use of ^(15)N stable isotope to quantify nitrogen transfer between mycorrhizal plants

Aims Mycorrhizas(fungal roots)play vital roles in plant nutrient acquisition,performance and productivity in terrestrial ecosystems.Arbuscular mycorrhizas(AM)and ectomycorrhizas(EM)are mostly important since soil nutrients,including NH+4,NO
3 and phosphorus,are translocated from mycorrhizal fungi to plants.Individual species,genera and even families of plants could be interconnected by mycorrhizal mycelia to form common mycorrhizal networks(CMNs).The function of CMNs is to provide pathways for movement or transfer of nutrients from one plant to another.In the past four decades,both ^(15)N external labeling or enrichment(usually expressed as atom%)and ^(15)N naturally occurring abundance(d^(15)N,&)techniques have been employed to trace the direction and magnitude of N transfer between plants,with their own advantages and limitations.Important Findings The heavier stable isotope ^(15)N is discriminated against 14N during biochemical,biogeochemical and physiological processes,due to a greater atomic mass.In general,non-N2-fixing plants had greater d^(15)N values than N2-fixing(;0&)ones.Foliar d^(15)N often varied by 5 to 10&in the order:non-mycorrhizas/AMs>EMs>ericoid mycorrhizas.Differences in d^(15)N(&)or ^(15)N(atom%)values could thus provide N transfer information between plants.A range of between 0 to 80%of one-way N transfer had been observed from N2-fixing mycorrhizal to non-N2-fixing mycorrhizal plants,but generally less than or around 10%in the reverse direction.Plant-to-plant N transfer may provide practical implications for plant performance in N-limited habitats.Considering that N translocation or cycling is crucial,and the potential benefits of N transfer are great in both agricultural and natural ecosystems,more research is warranted on either oneway or two-way N transfers mediated by CMNs with different species and under field conditions.

Agronomic evaluation of Herbaspirillum seropedicae strain ZAE94 as an inoculant to improve maize yield in Brazil

Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha^(-1) and was compared to the application of 40 and 80 kg N ha^(-1) without inoculation. Crop productivity and N accumulation in the grain were evaluated in addition to ^(15)N natural abundance(δ^(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha^(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha^(-1) and increased grain N content, especially in the off-season with 40 kg N ha^(-1). The inoculation treatments showed lower δ^(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season.