为探究中国新疆吐鲁番艾丁湖镇(后简称吐鲁番地区)小亚璃眼蜱的遗传进化特征及单倍型,本研究提取10份来自中国吐鲁番地区已经形态学鉴定为小亚璃眼蜱的DNA作为模板,采用本研究室设计的中国新疆地区优势璃眼蜱种16S r RNA基因通用引物,通...为探究中国新疆吐鲁番艾丁湖镇(后简称吐鲁番地区)小亚璃眼蜱的遗传进化特征及单倍型,本研究提取10份来自中国吐鲁番地区已经形态学鉴定为小亚璃眼蜱的DNA作为模板,采用本研究室设计的中国新疆地区优势璃眼蜱种16S r RNA基因通用引物,通过PCR进一步鉴定上述小亚璃眼蜱并测序;采用DNAMAN V 5.0软件分析吐鲁番地区10份小亚璃眼蜱16S r RNA基因与来自中国新疆(阿瓦提)、巴基斯坦、印度小亚璃眼蜱相应基因的同源性;经MEGA X软件构建小亚璃眼蜱与上述地区来源的小亚璃眼蜱16S r RNA基因的系统发育树,分析小亚璃眼蜱种的亲缘性和遗传多样性。PCR结果显示,10份小亚璃眼蜱均扩增出388 bp的目的基因片段,经BLAST比对结果显示10份样品均为小亚璃眼蜱;16S r RNA基因序列的同源性分析结果显示,本研究采集的10份小亚璃眼蜱之间的同源性最高,达99.57%,与来自巴基斯坦、印度、中国新疆(阿瓦提株MG651939)小亚璃眼蜱的同源性分别为99.55%、99.17%及99.11%;16S r RNA基因的遗传进化分析结果显示,中国新疆吐鲁番的小亚璃眼蜱1、3、9及10聚为一小支,遗传距离较近;小亚璃眼蜱2、4~8与巴基斯坦、印度及中国新疆小亚璃眼蜱聚为一大支,遗传距离较近。提示,中国吐鲁番地区的小亚璃眼蜱可能来自巴基斯坦。采用DNA SP V5.0软件统计吐鲁番及上述国家或地区小亚璃眼蜱的单倍型数量(Np)、单倍型多样性(Hd)及核甘酸多样性(pi);采用Popart v1.7软件构建吐鲁番及上述国家或地区小亚璃眼蜱的单倍型网络图。结果显示,来自4个不同国家或地区小亚璃眼蜱的Np为8,分别为Hap_1~Hap_8,Hd为0.572、pi为0.01156。与其他3个地区相比,中国吐鲁番地区小亚璃眼蜱的Np最高为7、Hd为0.911、pi为0.02593。小亚璃眼蜱单倍型网络图显示,在4个国家或地区小亚璃眼蜱仅共享单倍型Hap_1,其他3个地区存在单倍型Hap_2~Hap_8。中国吐鲁番地区小亚璃眼蜱不仅共享单倍型Hap_1,还存在单倍型Hap_3~Hap_8。上述结果表明吐鲁番地区小亚璃眼蜱可能来源于中国新疆阿瓦提地区及巴基斯坦,且其遗传多样性较为丰富。本研究为了解蜱种群间的种属鉴定和遗传进化关系等提供参考依据。展开更多
Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyz...Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyze the functions of differential nitrogen application rates including low(N1),medium(N2),and high(N3)levels in soil microbial community structure(bacterial and fungal)at 2 diverse soil depths(0-20,20-40 cm)through high-throughput sequencing technology by targeting 16S RNA gene and ITS1&ITS2 regions.All the observed physicochemical parameters exhibited significant improvement(p<0.05)with increased levels of nitrogen and the highest values for most parameters were observed at N2.However,pH decreased(p<0.05)gradually.The alpha and beta diversity analyses for bacterial and fungal communities’metagenome displayed more similarities than differences among all groups.The top bacterial and fungal phyla and genera suggested no obvious(p>0.05)differences among three group treatments(N1,N2,and N3).Furthermore,the functional enrichment analysis demonstrated significant(p<0.05)enrichment of quorum sensing,cysteine and methionine metabolism,and transcriptional machinery for bacterial communities,while various saprotrophic functional roles for fungal communities.Conclusively,moderately reducing the use of N-supplemented fertilizers is conducive to increasing soil nitrogen utilization rate,which can contribute to sustainable agriculture practices through improved soil quality,and microbial community structure and functions.展开更多
This editorial reviews the molecular mechanisms underlying the roles of the long non-coding RNA(lncRNA)small nucleolar RNA host gene 16(SNHG16)in digestive system cancers based on two recent studies on lncRNAs in dige...This editorial reviews the molecular mechanisms underlying the roles of the long non-coding RNA(lncRNA)small nucleolar RNA host gene 16(SNHG16)in digestive system cancers based on two recent studies on lncRNAs in digestive system tumors.The first study,by Zhao et al,explored how hBD-1 affects colon cancer,via the lncRNA TCONS_00014506,by inhibiting mTOR and promoting autophagy.The second one,by Li et al,identified the lncRNA prion protein testis specific(PRNT)as a factor in oxaliplatin resistance by sponging ZNF184 to regulate HIPK2 and influence colorectal cancer progression and chemoresistance,suggesting PRNT as a potential therapeutic target for colorectal cancer.Both of these two articles discuss the mechanisms by which lncRNAs contribute to the development and progression of digestive system cancers.As a recent research hotspot,SNHG16 is a typical lncRNA that has been extensively studied for its association with digestive system cancers.The prevailing hypothesis is that SNHG16 participates in the development and progression of digestive system tumors by acting as a competing endogenous RNA,interacting with other proteins,regulating various genes,and affecting downstream target molecules.This review systematically examines the recently reported biological functions,related molecular mechanisms,and potential clinical significance of SNHG16 in various digestive system cancers,and explores the relationship between SNHG16 and digestive system cancers.The findings suggest that SNHG16 may serve as a potential biomarker and therapeutic target for human digestive system cancers.展开更多
文摘为探究中国新疆吐鲁番艾丁湖镇(后简称吐鲁番地区)小亚璃眼蜱的遗传进化特征及单倍型,本研究提取10份来自中国吐鲁番地区已经形态学鉴定为小亚璃眼蜱的DNA作为模板,采用本研究室设计的中国新疆地区优势璃眼蜱种16S r RNA基因通用引物,通过PCR进一步鉴定上述小亚璃眼蜱并测序;采用DNAMAN V 5.0软件分析吐鲁番地区10份小亚璃眼蜱16S r RNA基因与来自中国新疆(阿瓦提)、巴基斯坦、印度小亚璃眼蜱相应基因的同源性;经MEGA X软件构建小亚璃眼蜱与上述地区来源的小亚璃眼蜱16S r RNA基因的系统发育树,分析小亚璃眼蜱种的亲缘性和遗传多样性。PCR结果显示,10份小亚璃眼蜱均扩增出388 bp的目的基因片段,经BLAST比对结果显示10份样品均为小亚璃眼蜱;16S r RNA基因序列的同源性分析结果显示,本研究采集的10份小亚璃眼蜱之间的同源性最高,达99.57%,与来自巴基斯坦、印度、中国新疆(阿瓦提株MG651939)小亚璃眼蜱的同源性分别为99.55%、99.17%及99.11%;16S r RNA基因的遗传进化分析结果显示,中国新疆吐鲁番的小亚璃眼蜱1、3、9及10聚为一小支,遗传距离较近;小亚璃眼蜱2、4~8与巴基斯坦、印度及中国新疆小亚璃眼蜱聚为一大支,遗传距离较近。提示,中国吐鲁番地区的小亚璃眼蜱可能来自巴基斯坦。采用DNA SP V5.0软件统计吐鲁番及上述国家或地区小亚璃眼蜱的单倍型数量(Np)、单倍型多样性(Hd)及核甘酸多样性(pi);采用Popart v1.7软件构建吐鲁番及上述国家或地区小亚璃眼蜱的单倍型网络图。结果显示,来自4个不同国家或地区小亚璃眼蜱的Np为8,分别为Hap_1~Hap_8,Hd为0.572、pi为0.01156。与其他3个地区相比,中国吐鲁番地区小亚璃眼蜱的Np最高为7、Hd为0.911、pi为0.02593。小亚璃眼蜱单倍型网络图显示,在4个国家或地区小亚璃眼蜱仅共享单倍型Hap_1,其他3个地区存在单倍型Hap_2~Hap_8。中国吐鲁番地区小亚璃眼蜱不仅共享单倍型Hap_1,还存在单倍型Hap_3~Hap_8。上述结果表明吐鲁番地区小亚璃眼蜱可能来源于中国新疆阿瓦提地区及巴基斯坦,且其遗传多样性较为丰富。本研究为了解蜱种群间的种属鉴定和遗传进化关系等提供参考依据。
基金This work was funded by Ningxia Hui Autonomous Region Key Research and Development Project(2021BEF02004),Central Finance Forestry Reform and Development Fund“Forest Seed Cultivation”.
文摘Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyze the functions of differential nitrogen application rates including low(N1),medium(N2),and high(N3)levels in soil microbial community structure(bacterial and fungal)at 2 diverse soil depths(0-20,20-40 cm)through high-throughput sequencing technology by targeting 16S RNA gene and ITS1&ITS2 regions.All the observed physicochemical parameters exhibited significant improvement(p<0.05)with increased levels of nitrogen and the highest values for most parameters were observed at N2.However,pH decreased(p<0.05)gradually.The alpha and beta diversity analyses for bacterial and fungal communities’metagenome displayed more similarities than differences among all groups.The top bacterial and fungal phyla and genera suggested no obvious(p>0.05)differences among three group treatments(N1,N2,and N3).Furthermore,the functional enrichment analysis demonstrated significant(p<0.05)enrichment of quorum sensing,cysteine and methionine metabolism,and transcriptional machinery for bacterial communities,while various saprotrophic functional roles for fungal communities.Conclusively,moderately reducing the use of N-supplemented fertilizers is conducive to increasing soil nitrogen utilization rate,which can contribute to sustainable agriculture practices through improved soil quality,and microbial community structure and functions.
文摘This editorial reviews the molecular mechanisms underlying the roles of the long non-coding RNA(lncRNA)small nucleolar RNA host gene 16(SNHG16)in digestive system cancers based on two recent studies on lncRNAs in digestive system tumors.The first study,by Zhao et al,explored how hBD-1 affects colon cancer,via the lncRNA TCONS_00014506,by inhibiting mTOR and promoting autophagy.The second one,by Li et al,identified the lncRNA prion protein testis specific(PRNT)as a factor in oxaliplatin resistance by sponging ZNF184 to regulate HIPK2 and influence colorectal cancer progression and chemoresistance,suggesting PRNT as a potential therapeutic target for colorectal cancer.Both of these two articles discuss the mechanisms by which lncRNAs contribute to the development and progression of digestive system cancers.As a recent research hotspot,SNHG16 is a typical lncRNA that has been extensively studied for its association with digestive system cancers.The prevailing hypothesis is that SNHG16 participates in the development and progression of digestive system tumors by acting as a competing endogenous RNA,interacting with other proteins,regulating various genes,and affecting downstream target molecules.This review systematically examines the recently reported biological functions,related molecular mechanisms,and potential clinical significance of SNHG16 in various digestive system cancers,and explores the relationship between SNHG16 and digestive system cancers.The findings suggest that SNHG16 may serve as a potential biomarker and therapeutic target for human digestive system cancers.
