将养殖粪便进行资源化处理,尤其是将粪便堆肥发酵后变为生物肥料还田,具有重要的经济、社会和生态效益。之前关于细菌在堆肥过程中的研究,大部分采用实验室培养、分离、鉴定的方法,由于受培养方式的限制,仅能分析粪肥中有限的细菌类别。...将养殖粪便进行资源化处理,尤其是将粪便堆肥发酵后变为生物肥料还田,具有重要的经济、社会和生态效益。之前关于细菌在堆肥过程中的研究,大部分采用实验室培养、分离、鉴定的方法,由于受培养方式的限制,仅能分析粪肥中有限的细菌类别。16S r RNA基因作为生物物种的特征核酸序列,被认为是最适于细菌系统发育和分类鉴定研究的指标。本研究使用16S r RNA基因高通量测序技术,分析了牛粪自然发酵与添加益生菌剂发酵过程中细菌种群的多样性变化。结果表明,1)新鲜牛粪、自然发酵1个月、自然发酵6个月的牛粪中细菌种群并没有明显的变化规律,说明自然发酵过程主要依赖于新鲜牛粪中携带的细菌种群;2)添加益生菌发酵后,细菌种群明显不同于不自然发酵过程中的细菌种群,其中变形菌门(Proteobacteria)细菌显著增加,而厚壁菌门(Firmicutes)细菌显著减少,说明益生菌剂能够显著改变堆肥过程中的细菌种群。本研究对于理解牛粪堆肥过程、提高堆肥效果,以及新型堆肥益生菌剂的开发都具有重要意义。展开更多
To study the phylogeny of Hynobiidae, we amplified DNA fragments of 470 bp 16S ribosomal RNA (16S rRNA) gene on mitochondrial DNA from Ranodon sibiricus and Ranodon tsinpaensis. PCR products were cloned into PMD18 T v...To study the phylogeny of Hynobiidae, we amplified DNA fragments of 470 bp 16S ribosomal RNA (16S rRNA) gene on mitochondrial DNA from Ranodon sibiricus and Ranodon tsinpaensis. PCR products were cloned into PMD18 T vector after purification. These sequences were determined and deposited in the GenBank (accession numbers: AY373459 for Ranodon sibiricus, AY372534 for Ranodon tsinpaensis). By comparing the nucleotide differences of 16S ribosomal RNA sequences among Liua shihi,Pseudohynobius flavomaculatus and Batrachuperus genus from GenBank database,we analyzed the divergences and base substitution among these sequences with the MEGA software. The molecular results support that B tibetanus, B pinchonii and B karlschmidti are classified into three valid species. Liua shihi has closer phylogenetic relationships to Ranodon tsinpaensis than to other species. More our results reveal that Pseudohynobius flavomaculatus is not a synonym of Ranodon tsinpaensis. .展开更多
文摘将养殖粪便进行资源化处理,尤其是将粪便堆肥发酵后变为生物肥料还田,具有重要的经济、社会和生态效益。之前关于细菌在堆肥过程中的研究,大部分采用实验室培养、分离、鉴定的方法,由于受培养方式的限制,仅能分析粪肥中有限的细菌类别。16S r RNA基因作为生物物种的特征核酸序列,被认为是最适于细菌系统发育和分类鉴定研究的指标。本研究使用16S r RNA基因高通量测序技术,分析了牛粪自然发酵与添加益生菌剂发酵过程中细菌种群的多样性变化。结果表明,1)新鲜牛粪、自然发酵1个月、自然发酵6个月的牛粪中细菌种群并没有明显的变化规律,说明自然发酵过程主要依赖于新鲜牛粪中携带的细菌种群;2)添加益生菌发酵后,细菌种群明显不同于不自然发酵过程中的细菌种群,其中变形菌门(Proteobacteria)细菌显著增加,而厚壁菌门(Firmicutes)细菌显著减少,说明益生菌剂能够显著改变堆肥过程中的细菌种群。本研究对于理解牛粪堆肥过程、提高堆肥效果,以及新型堆肥益生菌剂的开发都具有重要意义。
文摘To study the phylogeny of Hynobiidae, we amplified DNA fragments of 470 bp 16S ribosomal RNA (16S rRNA) gene on mitochondrial DNA from Ranodon sibiricus and Ranodon tsinpaensis. PCR products were cloned into PMD18 T vector after purification. These sequences were determined and deposited in the GenBank (accession numbers: AY373459 for Ranodon sibiricus, AY372534 for Ranodon tsinpaensis). By comparing the nucleotide differences of 16S ribosomal RNA sequences among Liua shihi,Pseudohynobius flavomaculatus and Batrachuperus genus from GenBank database,we analyzed the divergences and base substitution among these sequences with the MEGA software. The molecular results support that B tibetanus, B pinchonii and B karlschmidti are classified into three valid species. Liua shihi has closer phylogenetic relationships to Ranodon tsinpaensis than to other species. More our results reveal that Pseudohynobius flavomaculatus is not a synonym of Ranodon tsinpaensis. .
