With conserved regions and regions of high variations, 16s rDNA is an important molecular basis for the biological species identification and system evolu- tion. The modem molecular biology with 16s rDNA as the primer...With conserved regions and regions of high variations, 16s rDNA is an important molecular basis for the biological species identification and system evolu- tion. The modem molecular biology with 16s rDNA as the primer can accurately re- veal the diversity of microorganisms species and inheritance, thereby 16s rDNA se- quence analysis has become the main basis for classification and identification of microorganisms. Having overcome the limitations of traditional microculture methods, this method is easy to operate, quick and accurate to detect with high sensitivity, making it widely apply to species identification, community comparative analysis, phytecoenogenesis and the assessment of population diversity. It is a objective classification method with high credibility.展开更多
Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the re...Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.展开更多
文摘With conserved regions and regions of high variations, 16s rDNA is an important molecular basis for the biological species identification and system evolu- tion. The modem molecular biology with 16s rDNA as the primer can accurately re- veal the diversity of microorganisms species and inheritance, thereby 16s rDNA se- quence analysis has become the main basis for classification and identification of microorganisms. Having overcome the limitations of traditional microculture methods, this method is easy to operate, quick and accurate to detect with high sensitivity, making it widely apply to species identification, community comparative analysis, phytecoenogenesis and the assessment of population diversity. It is a objective classification method with high credibility.
基金Project supported by the Huaqiao University Graduate Student Scientific Research Innovation Ability Cultivation Plan Projectsthe Major Program of Department of Science and Technology of Fujian Province(No.2012Y4009)+4 种基金the Science and Technology Planning Project of Xiamen(No.3502Z20123036)the Xiamen Southern Oceanographic Center(No.14GYY008NF08)the Construction Project for Yun Leung Laboratory for Molecular Diagnostics(No.14X30127)the Technology Planning Projects of Quanzhou Social Development Fields(No.2014Z24)the Major Support Research Project of National Key Colleges Construction of Quanzhou Medical College(No.2013A13),China
文摘Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.
