AIM: To investigate the prognostic value of chromosome 18q microsatellite alterations (MA) in stage Ⅱ colon cancer. METHODS: One hundred and six patients with sporadic stage Ⅱ colon cancer were enrolled in this stud...AIM: To investigate the prognostic value of chromosome 18q microsatellite alterations (MA) in stage Ⅱ colon cancer. METHODS: One hundred and six patients with sporadic stage Ⅱ colon cancer were enrolled in this study. DNA was extracted from formalin-fixed, paraffin-embedded tumor and adjacent normal mucosal tissue samples. MA, including loss of heterozygosity (LOH) and microsatellite instability (MSI), was analyzed by polymerase chain reaction, polyacrylamide gel-electrophoresis and DNA sequencing at 5 microsatellite loci on chromosome 18q (D18S474, D18S55, D18S58, D18S61 and D18S64).RESULTS: Among the 102 patients eligible for MA information, the overall frequencies of LOH, high and low frequency MSI/microsatellite stable were 49.0%, 17.6% and 82.4%, respectively. The high frequency of 18q-LOH was signif icantly associated with the poor 5-year overall survival (OS) (P=0.008) and disease free survival (P=0.006). High levels of MSI were significantly associated with a longer 5-year OS (P=0.045) while the higher frequency of 18q-LOH at the loci of D18S474 and D18S61 was significantly associated with a poorer 5-year OS (P=0.010 and 0.005, respectively). But multivariate analysis showed that only the frequency of 18q-LOH was significantly associated with the prognosis of the disease. CONCLUSION: High frequency of 18q-LOH is an independent prognostic factor indicating poor prognosis of the patients with stage Ⅱ colon cancer.展开更多
Backgroud Fluorine-18-fluorodeoxyglucose （18F-FDG） positron emission tomography imaging can be used to assess the treatment efficacy of chemotherapy and prognosis.The aim of this study was to determine the uptake ra...Backgroud Fluorine-18-fluorodeoxyglucose （18F-FDG） positron emission tomography imaging can be used to assess the treatment efficacy of chemotherapy and prognosis.The aim of this study was to determine the uptake rate of 18F-FDG in colon cancer HCT-116 cells,and to evaluate the treatment efficacy of chemotherapy,hyperthermia and thermo-chemotherapy through the uptake inhibition rate of 18F-FDG.Methods The uptake rate of 18F-FDG in HCT-116 ceils was determined at various experimental conditions.The inhibition rate of cell growth,uptake rate of 18F-FDG and uptake inhibition rate of l8F-FDG in HCT-116 cells treated with 5-fluorouracil （5-FU） at various concentrations were determined.In HCT-116 cells subjected to chemotherapy （5-FU,100 μg/ml）,hyperthermia （43℃,40 minutes） and thermo-chemotherapy for 24 hours,the inhibition rate of cell growth and uptake inhibition rate of 18F-FDG were determined; early apoptosis,the morphology and ultrastructure of HCT-116 cells were examined; and the contents of glucose and lactate dehydrogenase （LDH） in the cell culture medium of HCT-116 cells were determined.One-way analysis of variance （ANOVA） and correlation analyses were conducted by using SPSS 16.0 software.Results The uptake rate of 18F-FDG in HCT-116 cells was （44.25±2.19）%.Under the condition of adding 5-FU at various concentrations for 24 hours,the uptake rate of 18F-FDG was negatively correlated with 5-FU dosage （r=-0.879,P 〈0.01）; the inhibition rate of cell growth revealed a positive correlation with the uptake inhibition rate of 18F-FDG （r=0.831,P 〈0.01）.In HCT-116 cells subjected to hyperthermia,chemotherapy,and thermo-chemotherapy for 24 hours,the uptake inhibition rates of 18F-FDG were （12.94±2.80）%,（28.25±4.59）%,and （21.60±3.68）%,respectively.The early apoptotic rates of HCT-116 cells were （9.80±0.16）%,（19.80±2.40）%,and （15.70±1.80）%,respectively.Moreover,the contents of glucose and LDH in cell culture medium of HCT-116 cells after treatments were higher than those before treatment.Conclusion The uptake inhibition rate of 18F-FDG can be used for early evaluation of hyperthermia and 5-FU treatment efficacy on cancer cells although hyperthermia （43℃,40 minutes） does not reveal the synergistic effect on 5-FU at the low dosage.展开更多
Background Our previous study has reported that supplementation of oligosaccharide-based polymer enhances gut health and disease resistance of pigs infected with enterotoxigenic E.coli(ETEC)F18 in a manner similar to ...Background Our previous study has reported that supplementation of oligosaccharide-based polymer enhances gut health and disease resistance of pigs infected with enterotoxigenic E.coli(ETEC)F18 in a manner similar to carbadox.The objective of this study was to investigate the impacts of oligosaccharide-based polymer or antibiotic on the host metabolic profiles and colon microbiota of weaned pigs experimentally infected with ETEC F18.Results Multivariate analysis highlighted the differences in the metabolic profiles of serum and colon digesta which were predominantly found between pigs supplemented with oligosaccharide-based polymer and antibiotic.The relative abundance of metabolic markers of immune responses and nutrient metabolisms,such as amino acids and carbohydrates,were significantly differentiated between the oligosaccharide-based polymer and antibiotic groups(q<0.2 and fold change>2.0).In addition,pigs in antibiotic had a reduced(P<0.05)relative abundance of Lachnospiraceae and Lactobacillaceae,whereas had greater(P<0.05)Clostridiaceae and Streptococcaceae in the colon digesta on d 11 post-inoculation(PI)compared with d 5 PI.Conclusions The impact of oligosaccharide-based polymer on the metabolic and microbial profiles of pigs is not fully understood,and further exploration is needed.However,current research suggest that various mechanisms are involved in the enhanced disease resistance and performance in ETEC-challenged pigs by supplementing this polymer.展开更多
文摘AIM: To investigate the prognostic value of chromosome 18q microsatellite alterations (MA) in stage Ⅱ colon cancer. METHODS: One hundred and six patients with sporadic stage Ⅱ colon cancer were enrolled in this study. DNA was extracted from formalin-fixed, paraffin-embedded tumor and adjacent normal mucosal tissue samples. MA, including loss of heterozygosity (LOH) and microsatellite instability (MSI), was analyzed by polymerase chain reaction, polyacrylamide gel-electrophoresis and DNA sequencing at 5 microsatellite loci on chromosome 18q (D18S474, D18S55, D18S58, D18S61 and D18S64).RESULTS: Among the 102 patients eligible for MA information, the overall frequencies of LOH, high and low frequency MSI/microsatellite stable were 49.0%, 17.6% and 82.4%, respectively. The high frequency of 18q-LOH was signif icantly associated with the poor 5-year overall survival (OS) (P=0.008) and disease free survival (P=0.006). High levels of MSI were significantly associated with a longer 5-year OS (P=0.045) while the higher frequency of 18q-LOH at the loci of D18S474 and D18S61 was significantly associated with a poorer 5-year OS (P=0.010 and 0.005, respectively). But multivariate analysis showed that only the frequency of 18q-LOH was significantly associated with the prognosis of the disease. CONCLUSION: High frequency of 18q-LOH is an independent prognostic factor indicating poor prognosis of the patients with stage Ⅱ colon cancer.
文摘Backgroud Fluorine-18-fluorodeoxyglucose （18F-FDG） positron emission tomography imaging can be used to assess the treatment efficacy of chemotherapy and prognosis.The aim of this study was to determine the uptake rate of 18F-FDG in colon cancer HCT-116 cells,and to evaluate the treatment efficacy of chemotherapy,hyperthermia and thermo-chemotherapy through the uptake inhibition rate of 18F-FDG.Methods The uptake rate of 18F-FDG in HCT-116 ceils was determined at various experimental conditions.The inhibition rate of cell growth,uptake rate of 18F-FDG and uptake inhibition rate of l8F-FDG in HCT-116 cells treated with 5-fluorouracil （5-FU） at various concentrations were determined.In HCT-116 cells subjected to chemotherapy （5-FU,100 μg/ml）,hyperthermia （43℃,40 minutes） and thermo-chemotherapy for 24 hours,the inhibition rate of cell growth and uptake inhibition rate of 18F-FDG were determined; early apoptosis,the morphology and ultrastructure of HCT-116 cells were examined; and the contents of glucose and lactate dehydrogenase （LDH） in the cell culture medium of HCT-116 cells were determined.One-way analysis of variance （ANOVA） and correlation analyses were conducted by using SPSS 16.0 software.Results The uptake rate of 18F-FDG in HCT-116 cells was （44.25±2.19）%.Under the condition of adding 5-FU at various concentrations for 24 hours,the uptake rate of 18F-FDG was negatively correlated with 5-FU dosage （r=-0.879,P 〈0.01）; the inhibition rate of cell growth revealed a positive correlation with the uptake inhibition rate of 18F-FDG （r=0.831,P 〈0.01）.In HCT-116 cells subjected to hyperthermia,chemotherapy,and thermo-chemotherapy for 24 hours,the uptake inhibition rates of 18F-FDG were （12.94±2.80）%,（28.25±4.59）%,and （21.60±3.68）%,respectively.The early apoptotic rates of HCT-116 cells were （9.80±0.16）%,（19.80±2.40）%,and （15.70±1.80）%,respectively.Moreover,the contents of glucose and LDH in cell culture medium of HCT-116 cells after treatments were higher than those before treatment.Conclusion The uptake inhibition rate of 18F-FDG can be used for early evaluation of hyperthermia and 5-FU treatment efficacy on cancer cells although hyperthermia （43℃,40 minutes） does not reveal the synergistic effect on 5-FU at the low dosage.
基金supported by Pancosma SA,Geneva,Switzerland,Jastro & Shields Graduate Research Awardthe United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA),multistate projects W4002 and NC1202
文摘Background Our previous study has reported that supplementation of oligosaccharide-based polymer enhances gut health and disease resistance of pigs infected with enterotoxigenic E.coli(ETEC)F18 in a manner similar to carbadox.The objective of this study was to investigate the impacts of oligosaccharide-based polymer or antibiotic on the host metabolic profiles and colon microbiota of weaned pigs experimentally infected with ETEC F18.Results Multivariate analysis highlighted the differences in the metabolic profiles of serum and colon digesta which were predominantly found between pigs supplemented with oligosaccharide-based polymer and antibiotic.The relative abundance of metabolic markers of immune responses and nutrient metabolisms,such as amino acids and carbohydrates,were significantly differentiated between the oligosaccharide-based polymer and antibiotic groups(q<0.2 and fold change>2.0).In addition,pigs in antibiotic had a reduced(P<0.05)relative abundance of Lachnospiraceae and Lactobacillaceae,whereas had greater(P<0.05)Clostridiaceae and Streptococcaceae in the colon digesta on d 11 post-inoculation(PI)compared with d 5 PI.Conclusions The impact of oligosaccharide-based polymer on the metabolic and microbial profiles of pigs is not fully understood,and further exploration is needed.However,current research suggest that various mechanisms are involved in the enhanced disease resistance and performance in ETEC-challenged pigs by supplementing this polymer.
