为探讨外源基因人基质金属蛋白酶组织抑制物-1（human tissue inhibitor of metalloproteinase-1，hTIMP-1）基因在转基因小鼠家系染色体上的整合和精确定位，应用Southern印迹检测外源基因在染色体上整合的位点及拷贝数，结果表明，外...为探讨外源基因人基质金属蛋白酶组织抑制物-1（human tissue inhibitor of metalloproteinase-1，hTIMP-1）基因在转基因小鼠家系染色体上的整合和精确定位，应用Southern印迹检测外源基因在染色体上整合的位点及拷贝数，结果表明，外源基因是以单拷贝、单位点形式整合；应用荧光原位杂交（fluorescence in situ hybridization，FISH）技术检测F4～F20代转基因小鼠中外源基因的整合．结果证明，该家系转基因小鼠自F4代起是纯合子，外源基因整合在17号染色体E区；反向PCR法（Inverse PCR，IPCR）克隆出约3．8kb外源基因整合位点处的侧翼序列．分析表明，外源基因整合在17号染色体E1.3区，ALK（anaplastic lymphoma kinase，ALK）基因第23个内含子区域、结果提示，获得的转基因小鼠为纯系，外源基因hTIMP-1已稳定整合在转基因小鼠染色体上，并能遗传给后代．展开更多
In this paper,a hybrid lattice Boltzmann flux solver(LBFS)is proposed for simulation of viscous compressible flows.In the solver,the finite volume method is applied to solve the Navier-Stokes equations.Different from ...In this paper,a hybrid lattice Boltzmann flux solver(LBFS)is proposed for simulation of viscous compressible flows.In the solver,the finite volume method is applied to solve the Navier-Stokes equations.Different from conventional Navier-Stokes solvers,in this work,the inviscid flux across the cell interface is evaluated by local reconstruction of solution using one-dimensional lattice Boltzmann model,while the viscous flux is still approximated by conventional smooth function approximation.The present work overcomes the two major drawbacks of existing LBFS[28–31],which is used for simulation of inviscid flows.The first one is its ability to simulate viscous flows by including evaluation of viscous flux.The second one is its ability to effectively capture both strong shock waves and thin boundary layers through introduction of a switch function for evaluation of inviscid flux,which takes a value close to zero in the boundary layer and one around the strong shock wave.Numerical experiments demonstrate that the present solver can accurately and effectively simulate hypersonic viscous flows.展开更多
配电网状态监测系统建设是构建泛在电力物联网的重要内容,准确预测通信网络带宽对明确业务需求、优化资源配置具有重要意义。针对当前配用电基于排队论的通信带宽预测方法在混合业务并存时带宽计算过程复杂的局限性,提出了一种基于G/M/...配电网状态监测系统建设是构建泛在电力物联网的重要内容,准确预测通信网络带宽对明确业务需求、优化资源配置具有重要意义。针对当前配用电基于排队论的通信带宽预测方法在混合业务并存时带宽计算过程复杂的局限性,提出了一种基于G/M/1/N队列模型的通信带宽预测方法。从状态监测系统架构和数据业务特点出发,分析混合数据业务到达时间间隔的统计描述以及基本传输速率,并给出了满足服务质量(quality of service,QoS)要求的系统带宽最优化化求解方法。以典型配电网状态监测应用为例,讨论了业务带宽、服务质量和带宽利用率之间的量化评价过程;验证了配电网状态监测网络信息流的"小数据"特征,为低功耗广域物联网通信技术在配电网状态监测中的应用提供了理论支撑。展开更多
基金supported by the State Key Laboratory of Aerodynamics of China(No.SKLA201401).
文摘In this paper,a hybrid lattice Boltzmann flux solver(LBFS)is proposed for simulation of viscous compressible flows.In the solver,the finite volume method is applied to solve the Navier-Stokes equations.Different from conventional Navier-Stokes solvers,in this work,the inviscid flux across the cell interface is evaluated by local reconstruction of solution using one-dimensional lattice Boltzmann model,while the viscous flux is still approximated by conventional smooth function approximation.The present work overcomes the two major drawbacks of existing LBFS[28–31],which is used for simulation of inviscid flows.The first one is its ability to simulate viscous flows by including evaluation of viscous flux.The second one is its ability to effectively capture both strong shock waves and thin boundary layers through introduction of a switch function for evaluation of inviscid flux,which takes a value close to zero in the boundary layer and one around the strong shock wave.Numerical experiments demonstrate that the present solver can accurately and effectively simulate hypersonic viscous flows.
文摘配电网状态监测系统建设是构建泛在电力物联网的重要内容,准确预测通信网络带宽对明确业务需求、优化资源配置具有重要意义。针对当前配用电基于排队论的通信带宽预测方法在混合业务并存时带宽计算过程复杂的局限性,提出了一种基于G/M/1/N队列模型的通信带宽预测方法。从状态监测系统架构和数据业务特点出发,分析混合数据业务到达时间间隔的统计描述以及基本传输速率,并给出了满足服务质量(quality of service,QoS)要求的系统带宽最优化化求解方法。以典型配电网状态监测应用为例,讨论了业务带宽、服务质量和带宽利用率之间的量化评价过程;验证了配电网状态监测网络信息流的"小数据"特征,为低功耗广域物联网通信技术在配电网状态监测中的应用提供了理论支撑。
