Objective To study mRNA expression of the mediating-related genes in osteoblasts after exposure to Titanium-alloy particles and cytochalasin-D. Methods Human osteoblasts were isolated from trabecular fragments and exp...Objective To study mRNA expression of the mediating-related genes in osteoblasts after exposure to Titanium-alloy particles and cytochalasin-D. Methods Human osteoblasts were isolated from trabecular fragments and exposed to 0.01% (v/v) titanium-alloy particles for 8h. Pretreatment of osteoblasts with cytochalasin-D( 5μmol/L ) to prevent phagocytosis prior to the addition of Titanium-alloy particles, mRNA expression of RANKL, OPG, and IL-6 was semi-quantified by RT-PCR. Results Expressions of RANKL, OPG, and IL-6 mRNA at respective levels were found in osteoblasts under normal condition, which did not show significant changes in the samples treated with cytochalasin-D but without particles. Exposure of osteoblasts to particles after being treated with or without cytochalasin-D altered mRNA expression of these genes, however, an increase in the ratio of RANKL/OPG mRNA was only found in samples without cytochalasin-D treatment. Conclusion Changes of expression of RANKL, OPG, and IL-6 mRNA in osteoblasts after exposure to titanium-alloy particles were independent of phagocytosis, whereas phagocytosis has a direct effect on their ability to stimulate osteoclast differentiation and function.展开更多
文摘Objective To study mRNA expression of the mediating-related genes in osteoblasts after exposure to Titanium-alloy particles and cytochalasin-D. Methods Human osteoblasts were isolated from trabecular fragments and exposed to 0.01% (v/v) titanium-alloy particles for 8h. Pretreatment of osteoblasts with cytochalasin-D( 5μmol/L ) to prevent phagocytosis prior to the addition of Titanium-alloy particles, mRNA expression of RANKL, OPG, and IL-6 was semi-quantified by RT-PCR. Results Expressions of RANKL, OPG, and IL-6 mRNA at respective levels were found in osteoblasts under normal condition, which did not show significant changes in the samples treated with cytochalasin-D but without particles. Exposure of osteoblasts to particles after being treated with or without cytochalasin-D altered mRNA expression of these genes, however, an increase in the ratio of RANKL/OPG mRNA was only found in samples without cytochalasin-D treatment. Conclusion Changes of expression of RANKL, OPG, and IL-6 mRNA in osteoblasts after exposure to titanium-alloy particles were independent of phagocytosis, whereas phagocytosis has a direct effect on their ability to stimulate osteoclast differentiation and function.
