Human Herpes Virus Type 2 ( HSV2 ), Human Cytomegalovirus (HCMV) in the Male Genital Tract and Fertilization

The possibility of infection of the human male genital tract by human herpes virus type 2 (HSV2) or human cytomegalovirus (HCMV) is well established and their sexual transmission has been the object of many studies. Moreover, medically assisted procreation, which helps in numerous fertility problems, raises the question of new viral risks linked to the application of these new technologies. In this review, we shall consider current knowledge in terms of the presence of HSV 2 and HCMV in the different parts of the genital tract of immunocompetent or immunodepressed men. We shall also consider the possibility of viral transmission by the sexual act or by the various techniques used in medically assisted procreation. We shall describe studies in human beings and in animals.

Antiviral activity of Basidiomycete mycelia against influenza type A(serotype H1N1) and herpes simplex virus type 2 in cell culture

In this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type A(serotype H1N1) and herpes simplex virus type 2(HSV-2), strain BH. All 10 investigated mushroom species inhibited the reproduction of influenza virus strain A/FM/1/47(H1N1) in MDCK cells reducing the infectious titer by 2.0–6.0 lg ID50. Four species, Pleurotus ostreatus, Fomes fomentarius, Auriporia aurea, and Trametes versicolor, were also determined to be effective against HSV-2 strain BH in RK-13 cells, with similar levels of inhibition as for influenza. For some of the investigated mushroom species—Pleurotus eryngii, Lyophyllum shimeji, and Flammulina velutipes—this is the first report of an anti-influenza effect. This study also reports the first data on the medicinal properties of A. aurea, including anti-influenza and antiherpetic activities. T. versicolor 353 mycelium was found to have a high therapeutic index(324.67), and may be a promising material for the pharmaceutical industry as an anti-influenza and antiherpetic agent with low toxicity. Mycelia with antiviral activity were obtained in our investigation by bioconversion of agricultural wastes(amaranth flour after CO2 extraction), which would reduce the cost of the final product and solve some ecological problems.

SEARCH FOR HERPES SIMPLEX VIRUS TYPE2（HSV－2）AND HUMAN PAPILLOMAVIRUS（HPV）IN THE NORMAL AND ABNORMAL CERVICAL SAMPLES

The specimens of 111 cervical carcinomas. 68 chronic cervicitis and 43 normal cervical exfoliated epithelial cells were examined for the presence of HSV2 DNA sequences with DNA hybridization using HSV2 BgL Ⅱ N fragment probe labelled by 32PdCTP. The result showed that the infection rates of HSV2 in the samples of cervical cancer.chronic cervicitis and normal epithelial cells were 1 4. 41 %(16/111). 27.94%( 19/68) and 25.58% ( 11/43),respectively. It was implied that early stages carcinogenesis of cervical epithelial cells might be correlated with the HSV2 infection.Sixteen HSV 2 positive samples of cervical carcinomas were also examined for the presence of the sequences homologous to human papillomavirus (HPV) type 6B/11. 16 and 18 DNA using dot blot hybridization (Tm17℃). The result indicated that 13 out of 16 were HPV 16 DNA hybridization positive accounting for 81. 2% of all HSV-2 positive samples and none of them were positive for HPV type 6B/11 and 18. The result indicated that double infection of HSV 2 and HPV16 in the same cervical carcinoma tissues may provide a strong evidence of the viral synergistic interaction in the induction of female cervical

北京地区性传播疾病常见病原体感染现状分析

目的了解北京地区性传播疾病常见病原体的感染现状,为临床诊疗及优生优育提供流行病学依据。方法收集2017年1月至2019年3月北京佑安医院皮肤性病门诊1927例样本的沙眼衣原体(CT)、解脲脲原体(UU)、淋病奈瑟菌(NG)、单纯疱疹病毒2型(HSV2)的核酸检测结果,并进行统计分析。结果1927份样本,阳性率最高为UU(39.0%),其次为CT(11.3%)、NG(5.0%),HSV2最低(2.4%);混合感染主要类型有CT+UU(5.1%)、UU+NG(1.3%)、CT+NG(1.1%);女性UU阳性率高于男性,男性NG阳性率高于女性,差异均有统计学意义(P<0.05);女性患者CT、UU以≤30岁阳性率最高,差异具有统计学意义(P<0.05);不同临床诊断组的CT、UU和NG阳性率比较差异均有统计学意义(P<0.05);梅毒、尖锐湿疣、HPV感染、阴道炎患者中UU与CT是主要病原体,尿道炎患者UU与NG为主要病原体;阴道炎患者≤30岁CT感染率最高;UU阳性组CT阳性率明显高于UU阴性组,NG阳性率则明显低于UU阴性组,而CT阳性组UU、NG阳性率明显高于CT阴性组,差异均有统计学意义(P<0.05)。结论性病患者UU检出率最高,有健康携带,治疗需结合临床;CT的检出率高于NG,女性CT与UU感染率呈年轻化;CT与UU,CT与NG易共感染,临床应进行多种病原联合检测,以防漏检,并加大筛查力度,防止无症状携带人群传播。