AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracel...AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracellular matrix(ECM).METHODS:HLECs were treated with TGF-β2(0,0.5,1.0,5,10μg/L)and CTGF(0,15,30,60,100μg/L)for different times(0,24,48,72h)in vitro and the expression ofα-smooth muscle actin(α-SMA),the main component of the extracellular matrix typeⅠcollagen(Col-1)and fibronectin(Fn)were measured by using real-time polymerase chain reaction(PCR)and western-blot.RESULTS:TGF-β2 and CTGF significantly increased expression ofα-SMA mRNA and protein(P【0.05,P【0.001),Fn mRNA and protein(P【0.001),Col-1 mRNA and protein(P【0.001).TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dosedependent manner(P【0.05,P【0.001).TGF-β2 and CTGF could induce HLECs to expressα-SMA,Fn and Col-1 in time-dependent manner.Each time of TGF-β2and CTGF induced HELCs expression ofα-SMA,Fn,Col-1 mRNA and protein was significant increase compared with control(P【0.05,P【0.001).CONCLUSION:TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis.展开更多
Let Y be a closed 3-manifold such that all flat SU(2)-connections on Y are non-degenerate.In this article,we prove a Uhlenbeck-type compactness theorem on Y for stable flat SL(2,C)connections satisfying an L^(2)-bound...Let Y be a closed 3-manifold such that all flat SU(2)-connections on Y are non-degenerate.In this article,we prove a Uhlenbeck-type compactness theorem on Y for stable flat SL(2,C)connections satisfying an L^(2)-bound for the real curvature.Combining the compactness theorem and a result from[7],we prove that the moduli space of the stable flat SL(2,C)connections is disconnected under certain technical assumptions.展开更多
In this paper,we study the homotopy classification of continuous maps between two r-1 connected 2r dimensional topological manifolds M,N.If we assume some knowledge on the homotopy groups of spheres,then the complete ...In this paper,we study the homotopy classification of continuous maps between two r-1 connected 2r dimensional topological manifolds M,N.If we assume some knowledge on the homotopy groups of spheres,then the complete classification can be obtained from the homotopy invariants of M,N.We design an algorithm and compose a program to give explicit computations.展开更多
基金National Natural Science Foundation of China(No.81070721)Inernational Exchange Program of Shaanxi Province,China(No.2012kw-31)
文摘AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracellular matrix(ECM).METHODS:HLECs were treated with TGF-β2(0,0.5,1.0,5,10μg/L)and CTGF(0,15,30,60,100μg/L)for different times(0,24,48,72h)in vitro and the expression ofα-smooth muscle actin(α-SMA),the main component of the extracellular matrix typeⅠcollagen(Col-1)and fibronectin(Fn)were measured by using real-time polymerase chain reaction(PCR)and western-blot.RESULTS:TGF-β2 and CTGF significantly increased expression ofα-SMA mRNA and protein(P【0.05,P【0.001),Fn mRNA and protein(P【0.001),Col-1 mRNA and protein(P【0.001).TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dosedependent manner(P【0.05,P【0.001).TGF-β2 and CTGF could induce HLECs to expressα-SMA,Fn and Col-1 in time-dependent manner.Each time of TGF-β2and CTGF induced HELCs expression ofα-SMA,Fn,Col-1 mRNA and protein was significant increase compared with control(P【0.05,P【0.001).CONCLUSION:TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis.
基金supported in part by NSF of China(11801539)the Fundamental Research Funds of the Central Universities(WK3470000019)the USTC Research Funds of the Double First-Class Initiative(YD3470002002)。
文摘Let Y be a closed 3-manifold such that all flat SU(2)-connections on Y are non-degenerate.In this article,we prove a Uhlenbeck-type compactness theorem on Y for stable flat SL(2,C)connections satisfying an L^(2)-bound for the real curvature.Combining the compactness theorem and a result from[7],we prove that the moduli space of the stable flat SL(2,C)connections is disconnected under certain technical assumptions.
基金the National Natural Science Foundation of China(Grant No.10671018)
文摘In this paper,we study the homotopy classification of continuous maps between two r-1 connected 2r dimensional topological manifolds M,N.If we assume some knowledge on the homotopy groups of spheres,then the complete classification can be obtained from the homotopy invariants of M,N.We design an algorithm and compose a program to give explicit computations.
