As a part of our interest in biologically active germacranolides from the genus Carpesium （Compositae）, we have investigated the constituents of Carpesium cernuum. This paper reports the isolation and structural elu...As a part of our interest in biologically active germacranolides from the genus Carpesium （Compositae）, we have investigated the constituents of Carpesium cernuum. This paper reports the isolation and structural elucidation of a new germacranolide, cernolide A （Compound 1）, from the herb. The structure of Compound 1 was determined as 2α,3β-dihydroxy-9-angeloxygermacra-4-en-6,12-olide on the basis of spectral evidence. The skeleton of Compound 1 was elucidation by IR, MS, ^1H and ^13C NMR, COSY, HMQC and HMBC experiments. The stereochemistry of Compound 1 was deduced by ROESY spectral data. Finally, the procedures of extraction and isolation were described in detail.展开更多
A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of s...A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of spectral analysis.展开更多
A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based o...A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based on spectroscopic evidence including 2D NMR techniques.展开更多
A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots o...A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots of Stellera chamaejasme L. together with known chamaechromone. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.展开更多
A convenient and stereoselective approach to the synthesis of(3S. 4R. 6S)- 2-oxo-3, 4-dihydroxy-6-methyl-octanate derivative, a key intermediate for the synthesis of HIV-1 protease inhibitor of didemnaketals analogue,...A convenient and stereoselective approach to the synthesis of(3S. 4R. 6S)- 2-oxo-3, 4-dihydroxy-6-methyl-octanate derivative, a key intermediate for the synthesis of HIV-1 protease inhibitor of didemnaketals analogue, has been developed successfully from L-(-)-menthone.展开更多
Objective:To isolate and characterize the cytotoxic compounds from Diospyros quercina(Baill.)G.E.Schatz&Lowry(Ebenaceae).Methods:An ethno-botanical survey was conducted in the south of Madagascar from July to Augu...Objective:To isolate and characterize the cytotoxic compounds from Diospyros quercina(Baill.)G.E.Schatz&Lowry(Ebenaceae).Methods:An ethno-botanical survey was conducted in the south of Madagascar from July to August 2010.Bio-guided fractionation assay was carried out on the root bark of Diospyros quercina,using cytotoxicity bioassay on murine P388 leukemia cell lines as model.The structures of the cytotoxic compounds were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry.Results:Biological experiments resulted in the isolation of three bioactive pure compounds(named TR-21,TR-22,and TR-23)which exhibited very good in vitro cytotoxic activities with the IC_(50)values of(0.017 5±0.0060)μg/mL,(0.089±0.005)μg/mL and(1.027±0.070)μg/mL respectively.Thus,they support the claims of traditional healers and suggest the possible correlation between the chemical composition of this plant and its wide use in Malagasy folk medicine to treat cancer.Conclusions:The ability of isolated compounds in this study to inhibit cell growth may represent a rational explanation for the use of Diospyros quercina root bark in treating cancer by Malagasy traditional healers.Further studies are,therefore,necessary to evaluate the in vivo antineoplastic activity of these cytotoxic compounds as effective anticancer drugs.展开更多
文摘As a part of our interest in biologically active germacranolides from the genus Carpesium （Compositae）, we have investigated the constituents of Carpesium cernuum. This paper reports the isolation and structural elucidation of a new germacranolide, cernolide A （Compound 1）, from the herb. The structure of Compound 1 was determined as 2α,3β-dihydroxy-9-angeloxygermacra-4-en-6,12-olide on the basis of spectral evidence. The skeleton of Compound 1 was elucidation by IR, MS, ^1H and ^13C NMR, COSY, HMQC and HMBC experiments. The stereochemistry of Compound 1 was deduced by ROESY spectral data. Finally, the procedures of extraction and isolation were described in detail.
基金the Jiangsu Province Basic Facility Project(Nos.:BM2006104,BM2006507).
文摘A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of spectral analysis.
文摘A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based on spectroscopic evidence including 2D NMR techniques.
文摘A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots of Stellera chamaejasme L. together with known chamaechromone. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.
文摘A convenient and stereoselective approach to the synthesis of(3S. 4R. 6S)- 2-oxo-3, 4-dihydroxy-6-methyl-octanate derivative, a key intermediate for the synthesis of HIV-1 protease inhibitor of didemnaketals analogue, has been developed successfully from L-(-)-menthone.
基金Supported by the Third World Academy of Science.(TWAS Fellowship for Research and Advanced Training FR number:Grant No.3240224121the International Foundation for Science (IFS,Stockholm,Sweden) and the Organizationfor the Prohibition of Chemical Weapons(OPCW)(IFS Research Grant No F/4921-2)
文摘Objective:To isolate and characterize the cytotoxic compounds from Diospyros quercina(Baill.)G.E.Schatz&Lowry(Ebenaceae).Methods:An ethno-botanical survey was conducted in the south of Madagascar from July to August 2010.Bio-guided fractionation assay was carried out on the root bark of Diospyros quercina,using cytotoxicity bioassay on murine P388 leukemia cell lines as model.The structures of the cytotoxic compounds were elucidated by 1D and 2D NMR spectroscopy and mass spectrometry.Results:Biological experiments resulted in the isolation of three bioactive pure compounds(named TR-21,TR-22,and TR-23)which exhibited very good in vitro cytotoxic activities with the IC_(50)values of(0.017 5±0.0060)μg/mL,(0.089±0.005)μg/mL and(1.027±0.070)μg/mL respectively.Thus,they support the claims of traditional healers and suggest the possible correlation between the chemical composition of this plant and its wide use in Malagasy folk medicine to treat cancer.Conclusions:The ability of isolated compounds in this study to inhibit cell growth may represent a rational explanation for the use of Diospyros quercina root bark in treating cancer by Malagasy traditional healers.Further studies are,therefore,necessary to evaluate the in vivo antineoplastic activity of these cytotoxic compounds as effective anticancer drugs.
