目的:对草龙Ludwigia hyssopifoLia(G.Don)Exell药材的质量控制进行研究,为草龙质量标准的制定提供科学依据。方法:采用HP L C法定量测定其中2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌...目的:对草龙Ludwigia hyssopifoLia(G.Don)Exell药材的质量控制进行研究,为草龙质量标准的制定提供科学依据。方法:采用HP L C法定量测定其中2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇的含量,以COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)为色谱柱;流动相:乙腈(B)-水(A),梯度洗脱(0~18min、29%B;18~45min、53%B);体积流量:1mL/min;检测波长:210nm;柱温:30℃。结果:2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇分别0.0499~0.7982μg(r=0.9995)、0.2000~3.1997μg(r=0.9999)、0.5001~8.0019μg(r=0.9997)线性关系良好,平均回收率分别为100.43%(RSD=1.43%)、100.03%(RSD=1.24%)、100.04%(RSD=1.47%)。结论:该方法准确、可靠、重复性好,可用于草龙药材的质量控制。展开更多
为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等...为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等波谱数据,结合参考文献,鉴定化合物的结构。结果表明,从长叶香茶菜地上部分分离得到12个三萜类化合物,它们分别是木栓醇(1)、齐墩果酸(2)、3β-羟基-齐墩果烷-11,13(18)-二烯-28酸(3)、2α,3α-二羟基齐墩果-12-烯-28酸(4)、2 a,3a,24-三羟基-12-烯-28-齐墩果酸(5)、2α,3β,24-三羟基齐墩果-12-烯-28酸(6)、cleistocalyxin(7)、熊果醇(8)、2α-羟基乌苏酸(9)、2a,3a,24-三羟基熊果-12,20(30)-二烯-28-酸甲酯(10)、maquatic acid(11)和2α,3α,24-三羟基-12,20(30)-二烯乌苏酸(12)。以上得到的三萜类化合物均为首次从该植物中分离得到。展开更多
AIM: To isolate the chemical constituents from the fruits of Hovenia acerba. METHODS: Compounds were isolated with stratography and solvent,and their structures were identified by spectroscopy. RESULTS: Five compounds...AIM: To isolate the chemical constituents from the fruits of Hovenia acerba. METHODS: Compounds were isolated with stratography and solvent,and their structures were identified by spectroscopy. RESULTS: Five compounds were identified as β-sitoseterol, betulin,3β-hydroxy olean-18(19)-en-28-oic acid, 2α,3β-dihydroxy-lup-20(29)-en-28-oic acid and daucosterol. CONCLUSION: The five compounds were obtained from Hovenia Thunb. for the frist time.展开更多
From the fruit of Rosa davidii Crep., eleven compounds were isolated and identified by spectral evidence, viz. 2 alpha, 3 beta, 19 beta -trihydroxyl-olean-12-en-28-oic acid (1), 2 alpha, 3 beta -dihydroxyl-urs-28 (13)...From the fruit of Rosa davidii Crep., eleven compounds were isolated and identified by spectral evidence, viz. 2 alpha, 3 beta, 19 beta -trihydroxyl-olean-12-en-28-oic acid (1), 2 alpha, 3 beta -dihydroxyl-urs-28 (13)-lactone (2), arjunic acid (3), euscaphic acid (4), 2 alpha, 3 beta -dihydroxyl-urs-12-en-28-oic acid (5), oleanolic acid (6), kaempferol (7), tiliroside (8), quercetin (9), daucosterol (10) and beta -sitosterol (11). Among them, 1 and 2 were new compounds.展开更多
文摘目的:对草龙Ludwigia hyssopifoLia(G.Don)Exell药材的质量控制进行研究,为草龙质量标准的制定提供科学依据。方法:采用HP L C法定量测定其中2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇的含量,以COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)为色谱柱;流动相:乙腈(B)-水(A),梯度洗脱(0~18min、29%B;18~45min、53%B);体积流量:1mL/min;检测波长:210nm;柱温:30℃。结果:2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇分别0.0499~0.7982μg(r=0.9995)、0.2000~3.1997μg(r=0.9999)、0.5001~8.0019μg(r=0.9997)线性关系良好,平均回收率分别为100.43%(RSD=1.43%)、100.03%(RSD=1.24%)、100.04%(RSD=1.47%)。结论:该方法准确、可靠、重复性好,可用于草龙药材的质量控制。
文摘为研究长叶香茶菜Rabdosia stracheyi(Benth ex Hook.)Hara的化学成分,本文采用硅胶、反相硅胶C 18、葡聚糖凝胶Sephadex LH-20、反相半制备高效液相等色谱方法对长叶香茶菜地上部分进行分离、纯化,利用1 H NMR、13 C NMR、HR-ESI-MS等波谱数据,结合参考文献,鉴定化合物的结构。结果表明,从长叶香茶菜地上部分分离得到12个三萜类化合物,它们分别是木栓醇(1)、齐墩果酸(2)、3β-羟基-齐墩果烷-11,13(18)-二烯-28酸(3)、2α,3α-二羟基齐墩果-12-烯-28酸(4)、2 a,3a,24-三羟基-12-烯-28-齐墩果酸(5)、2α,3β,24-三羟基齐墩果-12-烯-28酸(6)、cleistocalyxin(7)、熊果醇(8)、2α-羟基乌苏酸(9)、2a,3a,24-三羟基熊果-12,20(30)-二烯-28-酸甲酯(10)、maquatic acid(11)和2α,3α,24-三羟基-12,20(30)-二烯乌苏酸(12)。以上得到的三萜类化合物均为首次从该植物中分离得到。
文摘AIM: To isolate the chemical constituents from the fruits of Hovenia acerba. METHODS: Compounds were isolated with stratography and solvent,and their structures were identified by spectroscopy. RESULTS: Five compounds were identified as β-sitoseterol, betulin,3β-hydroxy olean-18(19)-en-28-oic acid, 2α,3β-dihydroxy-lup-20(29)-en-28-oic acid and daucosterol. CONCLUSION: The five compounds were obtained from Hovenia Thunb. for the frist time.
