OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).MET...OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.展开更多
基金Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission(10411966200)the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau(2009s023)the Shanghai Leading Academic Discipline Project(S30205)
文摘OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
